Cell three-dimensional culture automatic generation device

Abstract

The invention discloses a three-dimensional cell cultivation automation generating apparatus which comprises a main case, a negative plate, a first robot arm and a second robot arm. A work chamber and a storage chamber are arranged in the main case in a partition manner, a touch screen is arranged at the upper end of the main case, and a programmable high-voltage power supply is arranged in the storage chamber; a culture vessel containing a saline solution is placed on the negative plate; a needle clamp is arranged on the first robot arm in an insulated manner, and an injection needle is mounted in the needle clamp; an injector is arranged on the front side of the second robot arm, the promote end of the injector is in contact with the moving end of the second robot arm, and the injector is connected with the injection needle through a catheter; the needle clamp is connected with the high-voltage power supply, and the first robot arm, the second robot arm and the high-voltage power supply are controlled through the touch screen. The three-dimensional cell cultivation automation generating apparatus is safe and reliably, efficient and convenient and satisfactory to researchers of different fields.

Description

technical field [0001] The invention relates to the fields of cell biology, tissue engineering, biological materials and biotechnology, in particular to an automatic generating device for three-dimensional cell culture. Background technique [0002] It is well known that the three-dimensional cell culture technology has many advantages over the traditional two-dimensional culture. Researchers have confirmed this conclusion from the levels of metabolomics, protein expression, and nucleic acid. In the field of biotechnology, three-dimensional cell culture technology is often used, and biological researchers often use traditional two-dimensional cell culture models for research due to lack of knowledge in biomaterials, or use simple three-dimensional cell culture models such as hydrogels on the market. Cell culture products are not only expensive but also have many disadvantages. [0003] Therefore, researchers in tissue engineering and biomaterials use alginate-based hydrogel...

AI Technical Summary

Problems solved by technology

[0002] It is well known that the three-dimensional cell culture technology has many advantages over the traditional two-dimensional culture. Researchers have confirmed this conclusion from the levels of metabolomics, protein expression, and nucleic acid. In the field of biotechnology, three-dimensional cell culture technology is often used, and biological researchers often use traditional two-dimensional cell culture models for research due to lack of knowledge in biomaterials, or use simple three-dimensional cell culture models such as hydrogels on the market. Cell culture products are not only expensive but also have many disadvantages

[0003] Therefore, researchers in tissue engineering and biomaterials use alginate-based hydrogels, which are cheap, biocompatible and have many advantages, for three-dimensional culture, but they often lack professional tools. The cells on the edge of the scaffold and the cells on the edge of the scaffold are unscientific due to the uneven mass transfer factors

[0004] After discovering the problem, the advanced research team began to build the three-dimensional culture generating device by itself, but often due to lack of professional knowledge, the device was built using semi-automatic open patchwork, which was time-consuming and laborious, and involved high-voltage power supply, which was dangerous

Images