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Method for inhibiting browning of plant cell tissue in culture

A technology for plant cells and tissues, applied in the field of inhibiting browning of plant cells and tissues during cultivation, can solve the problems of toxic side effects, inability to use on a large scale, and incomplete suppression of browning, etc., to avoid damage, wide application range, prevent browning effect

Inactive Publication Date: 2017-03-08
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above deficiencies or improvement needs of the prior art, the present invention provides a method for inhibiting the browning of plant cell tissue in culture, by inhibiting the synthesis of flavonoids in plant cells in the culture process, fundamentally inhibiting browning, thus Solve the current technical problems of incomplete inhibition of browning, inability to use on a large scale, toxic side effects and complicated operations

Method used

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  • Method for inhibiting browning of plant cell tissue in culture

Examples

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Effect test

Embodiment 1

[0037] Comparative analysis of enzymatic browning-related characteristics between browning cell lines and non-browning cell lines of Taxus chinensis

[0038] (1) There are two cell lines preserved in the laboratory, one is the cell line (NA) which has been subcultured for half a year. The 10-year-old cell line (CA) basically did not have browning phenomenon during the solid subculture process. The two cell lines were inoculated on MS solid medium, sucrose 30g / L, the temperature was controlled at 25°C, and cultured in the dark. NA cells showed dark brown, and the cell growth state was not good, and CA cells showed beige, and the growth was vigorous ( figure 1 ).

[0039] (2) In order to explore the reasons for the differences in the degree of browning between the two cell lines, the flavonoid content and PPO activity of the two cell lines were compared and analyzed. The study found that the maximum difference in the content of flavonoids in the two cell lines was 17 times at...

Embodiment 2

[0040] The browning control of embodiment 2 Chinese yew browning cell lines

[0041] The cell line (NA) of subculture half a year in embodiment 1 is inoculated in the corresponding MS solid subculture medium that contains gibberellin as 10mg / L, 50mg / L and 100mg / L, sucrose 30g / L, temperature control Culture at 25°C in the dark, every 25 days is a subculture cycle, and replace with fresh above-mentioned subculture medium. After two cycles of cultivation, the total flavonoid content in the cells decreased by 41%, 77% and 86% respectively, so that no browning occurred on the culture medium adding 50mg / L and 100mg / L gibberellin. Solid yew callus and yew cell solid cultures.

Embodiment 3

[0043] Induced Browning Control of Cotton Callus

[0044] Cotton callus was induced by MS medium, and the concentration of gibberellin was 50mg / L. Select hypocotyls of sterile cotton seedlings in good growth condition and cut them into small pieces of about 1 cm, and cut their cotyledons into small pieces of about 1 cm×1 cm, and place them on the surface of MS solid medium containing 50 mg / L gibberellin concentration. The culture temperature is 28° C., and the light is 12 hours a day, and the culture is 30 days. Adding 50mg / L gibberellin to MS medium finally reduced the content of total flavonoids in hypocotyls and cotyledons by 58% and 67%, respectively, thus obtaining cotton calli without browning.

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Abstract

The invention discloses a method for inhibiting browning of plant cell tissue in culture. The method includes that the plant cell tissue is cultured under following conditions, and synthetic amount of flavonoids is reduced by more than 30% during culture of the plant cell tissue under the conditions. The method aims at the plant cell tissue of plant callus tissue cultures, plant-cell solid cultures, plant-cell liquid cultures, plant-cell reactor liquid cultures and the like. Compared with an existing traditional method, the method has the advantages of being more targeted, obviously in browning control effect, suitable for industrialized application of large-scale plant cell culture and broad in market prospect.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for inhibiting browning of plant cell tissue during culture. Background technique [0002] Plant cell tissue culture technology has achieved huge economic, social and ecological benefits in terms of rapid plant reproduction, detoxification, accelerated breeding process, production of secondary metabolites, and preservation of germplasm resources. Due to its superiority, plant cell culture technology is still expanding its application fields. At present, the application of plant cell culture technology to produce exogenous animal protein has become a hot spot in global research and application. However, the problem of browning still commonly exists in the field of plant cell tissue culture, which seriously affects the growth of plant cell tissue, and even leads to its death, thereby reducing the accumulation of target metabolites or other active s...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 余龙江董艳山付春华苏鹏
Owner HUAZHONG UNIV OF SCI & TECH
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