Production method of magnetic immobolized enzyme

An immobilized enzyme, production method technology, applied in biochemical equipment and methods, immobilized on or in inorganic carriers, glycosylase and other directions, can solve the problem that magnetic materials are not easy to disperse, industrial safety production hazards, magnetic materials cannot be Effectively achieve uniform inclusion and other problems to avoid the decrease of reaction activity, avoid the gel speed is too slow, and avoid the gel transfer that is not easy

Pending Publication Date: 2017-03-08
厦门庚能新材料技术有限公司
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Problems solved by technology

[0004] 1. In view of the large amount of heat generated during the reaction process, the reaction at room temperature will affect the production safety of the system, so how to avoid the accumulation of heat should be considered in industrial production; at the same time, if the reaction solution is added at one time, due to the reaction If the speed becomes faster, it will also cause the reaction to be too violent, which will also cause harm to the safe production of the industry;
[0005] 2. If magnetic material is added for clathration before adding alkaline solution for neutralization, due to the low viscosity and high acidity of the system, the magneti

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  • Production method of magnetic immobolized enzyme

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[0029] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer and more comprehensible, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

[0030] Embodiments of the present invention employ figure 1 The equipment shown, and the magnetic immobilized enzyme was prepared by the following steps:

[0031] Step 1: Weigh the following components by mass: 100 parts of the reaction substrate, 100 parts of the template solution, 2 parts of ferric oxide powder, 20 parts of cellulase; the reaction substrates are tetraethyl orthosilicate and dimethyl phthalate disiloxane, in which tetraethylorthosilicate accounts for 80% by mass and dimethyldisiloxane accounts for 20% by mass; the temp...

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Abstract

The invention discloses a production method of a magnetic immobolized enzyme. The method comprises a sol formation step by reaction substrates, a magnetic material and enzyme inclusion, a gelling step and the like. The production process of the magnetic immobolized enzyme is realized; the production safety and the production operability are improved; the production process is simplified; meanwhile, the magnetic inclusion and the gelling are more uniform; the enzyme stability of the magnetic immobolized enzyme is improved.

Description

technical field [0001] The invention relates to the field of production methods, in particular to the field of a production method of magnetic immobilized enzyme. Background technique [0002] In the Chinese invention patent application CN104498472A published on April 8, 2015, a magnetically immobilized cellobiase nano-mesoporous material and its preparation method are described. The method realizes the reproducibility of the enzyme by immobilizing the enzyme. Use, and improve the operability of enzyme recovery by inclusion of magnetic materials. [0003] But in this invention patent, the production process of immobilized enzyme is not disclosed. Expanding from laboratory to industrial production is a complex and arduous project. Differences in process schemes will cause differences in product performance, which is also a characteristic of industrial production. The practical problems in industrial production for the preparation method disclosed in the patent application C...

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Application Information

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IPC IPC(8): C12N9/42C12N11/14
CPCC12N11/14C12N9/2437C12Y302/01004
Inventor 危岩孙亚飞于伟娜叶培荣童龙欢
Owner 厦门庚能新材料技术有限公司
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