Production method of magnetic immobolized enzyme

Abstract

The invention discloses a production method of a magnetic immobolized enzyme. The method comprises a sol formation step by reaction substrates, a magnetic material and enzyme inclusion, a gelling step and the like. The production process of the magnetic immobolized enzyme is realized; the production safety and the production operability are improved; the production process is simplified; meanwhile, the magnetic inclusion and the gelling are more uniform; the enzyme stability of the magnetic immobolized enzyme is improved.

Description

technical field [0001] The invention relates to the field of production methods, in particular to the field of a production method of magnetic immobilized enzyme. Background technique [0002] In the Chinese invention patent application CN104498472A published on April 8, 2015, a magnetically immobilized cellobiase nano-mesoporous material and its preparation method are described. The method realizes the reproducibility of the enzyme by immobilizing the enzyme. Use, and improve the operability of enzyme recovery by inclusion of magnetic materials. [0003] But in this invention patent, the production process of immobilized enzyme is not disclosed. Expanding from laboratory to industrial production is a complex and arduous project. Differences in process schemes will cause differences in product performance, which is also a characteristic of industrial production. The practical problems in industrial production for the preparation method disclosed in the patent application C...

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Problems solved by technology

[0004] 1. In view of the large amount of heat generated during the reaction process, the reaction at room temperature will affect the production safety of the system, so how to avoid the accumulation of heat should be considered in industrial production; at the same time, if the reaction solution is added at one time, due to the reaction If the speed becomes faster, it will also cause the reaction to be too violent, which will also cause harm to the safe production of the industry;

[0005] 2. If magnetic material is added for clathration before adding alkaline solution for neutralization, due to the low viscosity and high acidity of the system, the magnetic material is prone to agglomeration. If the viscosity is too high, the magnetic material is not easy to disperse, which will easily cause the magnetic material to be unable to effectively achieve uniform inclusion;

[0006] 3. During the gelation process, if the gel is directly gelled under neutral conditions, the product will be covered in the reaction kettle and it will be difficult to take out, and at the same time, the gelation rate will be too fast to cause the problem of uneven gelation;

[0007] The immobilized enzymes produced are prone to enzyme inactivation, so it is also necessary to consider how to maintain the stability of the immobilized enzymes

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