Method for facilitating haematococcus pluvialis to produce astaxanthin at high temperature
A technology of Haematococcus pluvialis and astaxanthin, which is applied in the biological field, can solve the problems of high production cost, cell bleaching and death, etc., and achieves the effects of low cost, simple operation and increased yield
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Embodiment 1
[0016] (a) Green cell nutrient culture: Carry out green expansion culture of Haematococcus pluvialis with BG-11 full nutrient medium, temperature 22~24℃, light 50~100μmol / m 2 s, the feed contains 0.5~1.5% CO 2 After 7-10 days of culture, the supernatant culture solution was removed by sedimentation to obtain concentrated green cell algae liquid.
[0017] (b) Low-light and high-temperature induction: Dilute the concentrated algae solution to 0.19 g / L with BG-11 nitrogen-deficient medium, and inoculate it into an outdoor column-type thin-film photobioreactor with a light path of 5 cm and a height of 150 cm, fed with 1%~5% CO 2 The mixed air is used to control the pH at 7.0~8.0. The cultivation site is located in Weifang, Shandong, and the season is summer. Use the method of shading to control the light intensity at 50~200μE / m 2 / s. During the cultivation period, the temperature of the algae liquid is 30-40°C. Observed by microscope, under the condition of low light and hig...
Embodiment 2
[0021] Embodiment 2 is a control experiment group, and the cultivation of green vegetative cells is the same as that in Embodiment 1.
[0022] Low light and high temperature induction: the initial algae cell inoculation concentration was 0.15 g / L, and other culture conditions were the same as in Example 1.
[0023] Induction by high light and high temperature: After 3 days of culture, all the green algae cells turned into red sclerenchyma cells, and the content of astaxanthin was 1.51%. After the shading was removed, no temperature control measures were adopted, and the temperature of the algae liquid reached a maximum of 45°C during the cultivation process. It was harvested after 2 days of induced cultivation, and the content of astaxanthin was determined.
[0024] The results showed that when the induction ended, the cell density of Example 2 reached 0.43 g / L, and the astaxanthin content was 2.54%.
Embodiment 3
[0026] Embodiment 3 is a control experiment group, and the cultivation of green vegetative cells is the same as that in Embodiment 1.
[0027] High light and temperature control induction: the initial algae cell inoculation concentration is 0.16 g / L, the first stage of induction with low light and high temperature is not used, the whole process is not shaded, and the temperature is controlled at 20~25°C by spraying. Harvest after 7 days of induction culture, and determine the content of astaxanthin. The results showed that more bleached cells appeared at the initial stage of induction, and the cell density of Example 3 was 0.41 g / L at the end of induction, and the astaxanthin content was 2.23%.
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