A method suitable for promoting the production of astaxanthin by Haematococcus pluvialis under high temperature conditions

A technology of Haematococcus pluvialis and astaxanthin, applied in the biological field, can solve the problems of high production cost, cell bleaching and death, etc., and achieve the effect of low cost, simple operation, and reduced cooling cost

Active Publication Date: 2020-05-26
BIOALGO CY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially in the early stage of induction culture, green algae cells are relatively fragile, and exposure to extreme conditions of high light and high temperature can easily lead to cell bleaching and death
Therefore, in the large-scale breeding process, it is necessary to use cooling water to spray or circulate the reactor to cool down, and the cooling and circulation of condensed water will lead to high production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (a) Green cell nutrient culture: Carry out green expansion culture of Haematococcus pluvialis with BG-11 full nutrient medium, the temperature is 22~24 ℃, and the light is 50~100 μmol / m 2 s, the feed contains 0.5~1.5% CO 2 After 7-10 days of culture, the supernatant culture solution was removed by sedimentation to obtain concentrated green cell algae liquid.

[0017] (b) Low-light and high-temperature induction: Dilute the concentrated algae solution to 0.19 g / L with BG-11 nitrogen-deficient medium, and inoculate it into an outdoor column-type thin-film photobioreactor with a light path of 5 cm and a height of 150 cm, fed with 1%~5% CO 2 The mixed air is used to control the pH at 7.0~8.0. The cultivation site is located in Weifang, Shandong, and the season is summer. Use the method of shading to control the light intensity at 50~200μE / m 2 / s. During the cultivation period, the temperature of the algae liquid is 30-40°C. Observed under the microscope, under the con...

Embodiment 2

[0021] Embodiment 2 is a control experiment group, and the cultivation of green vegetative cells is the same as that in Embodiment 1.

[0022] Low light and high temperature induction: the initial algae cell inoculation concentration was 0.15 g / L, and other culture conditions were the same as in Example 1.

[0023] Induction by high light and high temperature: After 3 days of culture, all the green algae cells turned into red sclerenchyma cells, and the content of astaxanthin was 1.51%. After the shading was removed, no temperature control measures were adopted, and the temperature of the algae solution reached a maximum of 45°C during the cultivation process. After 2 days of induced cultivation, the algae were harvested and the content of astaxanthin was determined.

[0024] The results showed that when the induction ended, the cell density of Example 2 reached 0.43 g / L, and the astaxanthin content was 2.54%.

Embodiment 3

[0026] Embodiment 3 is a control experiment group, and the cultivation of green vegetative cells is the same as that in Embodiment 1.

[0027] High light and temperature control induction: the initial algae cell inoculation concentration is 0.16 g / L, the first stage of induction with low light and high temperature is not used, the whole process is not shaded, and the temperature is controlled at 20~25°C by spraying. Harvest after 7 days of induction culture, and determine the content of astaxanthin. The results showed that more bleached cells appeared at the initial stage of induction, and the cell density of Example 3 was 0.41 g / L at the end of induction, and the astaxanthin content was 2.23%.

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Abstract

The invention relates to a method for facilitating haematococcus pluvialis to produce astaxanthin at high temperature, and belongs to the technical field of biology. Under the condition of megathermal climate in summer, an inducing scheme with low inoculation density and low light intensity and at high temperature is used at first, green vegetative cells can be effectively stimulated and then converted into thick-walled red cells, the problem that green cells are easy to bleach and then die is solved, and high temperature tolerance and high light tolerance of algae cells are improved. The content of the astaxanthin is increased within a short time by high-light and temperature-controlled induction. The method is simple to operate and low in cost; and the yield of the astaxanthin through the haematococcus pluvialis under the condition of the megathermal climate in the summer can be increased remarkably.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular provides a method suitable for promoting the production of astaxanthin by Haematococcus pluvialis under high temperature conditions. Background technique [0002] Astaxanthin, the chemical name is 3,3'-dihydroxy-4,4'-diketonyl-β,β'-carotene, and the molecular formula is C 40 h 52 o 4 , with a relative molecular mass of 596.86, also known as astaxanthin, astaxanthin or lobster shell pigment, is a type of carotenoid and the highest level of carotenoid synthesis. Natural astaxanthin is the strongest antioxidant found in nature so far, known as "super oxidant". Astaxanthin has a wide range of applications. It can not only be used as a feed additive for aquaculture and human food additives, but also has great application potential in the fields of medicine, cosmetics and nutritional health products. Haematococcus pluvialis is the organism with the highest content of natural astaxanth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P23/00C12R1/89
CPCC12N1/12C12P23/00
Inventor 郑玉彬曹培鑫郑玉瑞代云法王华赵坤陈树林
Owner BIOALGO CY CO LTD
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