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An application of epigallocatechin gallate as a medicine for prawn white spot syndrome control

A technology of epigallocatechin and gallic acid ester, which is applied in the application field of drugs for preventing and treating white spot syndrome of prawns

Inactive Publication Date: 2017-03-15
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although shrimp white spot syndrome virus has been alleviated and controlled in some areas or farms, because most of these technologies are in the pilot test promotion stage and use costs and other issues, shrimp white spot syndrome virus disease is still an important disease affecting the shrimp farming industry

Method used

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  • An application of epigallocatechin gallate as a medicine for prawn white spot syndrome control
  • An application of epigallocatechin gallate as a medicine for prawn white spot syndrome control
  • An application of epigallocatechin gallate as a medicine for prawn white spot syndrome control

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Experimental program
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Effect test

Embodiment 1

[0017] Materials and Methods

[0018] Epigallocatechin gallate (EGCG) with a content of 98% was purchased from Wuhu Tianyuan Biotechnology Co., Ltd. Sterilized ultrapure water was prepared to prepare drug concentrations of 1 μg / mL, 5 μg / mL, 10 μg / mL, 20 μg / mL, and 40 μg / mL for use. The vannamei shrimp (Penaeus vannamei) used in the test is SPF grade 3-4g, and the WSSV virus is negative after testing. The breeding center temporarily raises it for 1 week. The shrimp with good swimming state, healthy body color and strong vitality are selected for the test.

[0019] The WSSV virus was isolated and purified in our laboratory, and a small amount of the virus suspension was observed with an electron microscope to confirm the virus particles. Dilute 1000 times with sterilized seawater for use.

[0020] For the preparation of the injection, 50 μL of different concentrations of EGCG was added, and 10 μL of WSSV virus suspension was added to incubate at room temperature for 2 hours. S...

Embodiment 2

[0041] Adding an appropriate amount of epigallocatechin gallate to the conventional prawn feed formula can prevent and treat prawn white spot syndrome.

Embodiment 3

[0043] In the process of raising prawns, choose epigallocatechin gallate, catechin or tea polyphenols, and feed them with mixing materials.

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Abstract

The invention relates to a novel use of epigallocatechin gallate, namely an application of the epigallocatechin gallate in preparation of a medicine for prawn white spot syndrome control. The application is advantageous in that tests prove that the epigallocatechin gallate (EGCG) can antagonize invasion infection of WSSV viruses and can control the prawn white spot syndrome. Artificial infection tests prove that the death rate of prawns infected with the WSSV viruses shows a decreasing trend along with increases of the EGCG concentration, and the death rate of prawns when the EGCG concentration during toxic material elimination is 20 [mu]g / mL and 40 [mu]g / mL is only about 20%.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to the application of epigallocatechin gallate as a medicine for preventing and treating white spot syndrome of prawns. Background technique [0002] Prawn meat is delicious and nutritious, rich in trace elements such as calcium, magnesium, phosphorus, iodine, potassium, etc. It is one of the most popular aquatic products in the international aquatic product market. However, the high-density shrimp farming industry is also plagued by diseases, among which white spot syndrome virus (WSSV) is a serious disease among many diseases, and it is also a common problem faced by the shrimp farming industry in the world . WSSV belongs to the family Nimaviridae and the genus Whispovirus, an enveloped circular dsDNA virus of about 300 kb. WSSV has strong infectivity, high lethality, and wide host spectrum. It can infect not only prawns, but also freshwater and seawater crustaceans such as ...

Claims

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Application Information

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IPC IPC(8): A61K31/353A61P31/20
CPCA61K31/353
Inventor 吕利群王浩喻飞夏思瑶卢翠玉陈垚曲
Owner SHANGHAI OCEAN UNIV
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