A kind of preparation method of L-citrulline crude product and L-citrulline prepared therefrom

Abstract

The invention discloses a method for preparing an L-citrulline crude product and L-citrulline prepared through the method. The method for preparing the L-citrulline crude product includes the steps of preparing L-citrulline liquid, preparing the L-citrulline crude product from the L-citrulline liquid, refining the crude product, and obtaining an L-citrulline finished product. When the method is used for producing the L-citrulline crude product, no ethyl alcohol is used, so the method has the advantages that production conditions are mild, concentration time is short, product purity is high, yield is high and production cost is low.

Description

technical field [0001] The invention relates to the field of biochemical technology. More specifically, it relates to a method for preparing crude L-citrulline and L-citrulline prepared therefrom. Background technique [0002] In 1914, Uga Taro and others isolated citrulline for the first time from watermelon juice, and it was recognized as an amino acid by Wada Mitsutoku afterwards. Citrulline exists in the seeds of Cucurbitaceae plants in a free state. So far, people have successfully isolated citrulline H1 from watermelon juice, wild watermelon leaves, walnut kernels, walnut seedlings and seeds. Citrulline is a non-protein amino acid. Foreign scientists have done more research on citrulline, especially in Japan, the United States and Europe. In China, the research on citrulline is still in the stage of understanding, there is no corresponding report on the production method, and there are not many researches on its detection method and physiological function, only a sma...

AI Technical Summary

Problems solved by technology

[0007] (2) The difficulty of fermentation production is that the yield of L-citrulline per unit volume is low, the highest is only 1.7g / L, and the cost of extracting L-citrulline from the fermentation broth is relatively high

But this method needs a large amount of ethanol, and production cost increases, and adding ethanol also can cause harm to the health of front-line production personnel in addition, separate out the mother liquor after L-citrulline crude product in addition, also can't carry out crystallization again, because ethanol When separating from the mother liquor, a large amount of steam is needed, and the high temperature will damage the L-citrulline remaining in the mother liquor and produce a large amount of by-products. Even if resin is used, it is difficult to separate L-citrulline, which gives Actual production has brought inconvenience, so, for improving the technology in the step of preparing L-citrulline crude product, just seem particularly important for the actual production enterprise