111 results about "L-citrulline" patented technology

A composition for enhancing cellular energy that includes creatine, L-arginine-α-ketoglutarate, D-ribose, L-carnitine, L-citrulline, and pyruvate. The composition is administering to a subject to enhance cellular energy, to increase relative intensity of physical activity performed by the subject, to increase endurance of the subject during the physical activity and to increase the muscle mass of the subject.

The invention provides a broad-spectrum nitric oxide free radical health care product, comprising the following components by weight on a daily basis: 4-6g of L-arginine, 200-1000mg of L-citrulline, 1-25g of cooked soybean meal, 80-100mg of pycnogenol (pine bark extract), 100-150mg of grape seed extracts, 100-150mg of EGB761 (extract of ginkgo biloba), 500mg of curcumin, 500mg of vitamin C, 200-400mg of vitamin E, 20-30mg of beta-carotene, 800mu g of folic acid (containing calcium folinate) and 100mu g of selenium. The health care product not only has the effect of generating the nitric oxide free radical, but also can protect the activity and the stability of the nitric oxide free radical, provides corresponding health care and auxiliary treatment formulas according to different individuals to achieve obvious health care effect, and has specific curative effects on the diseases.

The invention provides an industrial method for extracting natural L-citrulline from plant tissues of watermelons or citrullus lamatus, and the like, which belongs to the technical field of extracting natural products in the food industry and the medicine industry. The watermelons, the citrullus lamtus or the stems and leaves thereof are taken as raw materials, and the extracting process comprises the following steps: firstly, the raw materials are washed clean and broken to separate the watermelon peel from the watermelon pulp for respectively extracting juice; the two juices are mixed, and micro-filtration and ultra-filtration are carried out to remove insoluble substances and macromolecular substances; and then reverse osmosis and nano-filtration are carried out to obtain the filtrate; the pH value of the filtrate is regulated and then a macroporous strong acid cation exchange resin column is added; and then ammonia water is taken as an eluant for isocratic elution; the eluent obtained is decompressed, distilled, deaminized, discolored, condensed and crystallized to obtain a white crystalline product of the natural L-citrulline extracted from the watermelons. The prepared product is a pure natural product without harmful substances and has high purity and good quality, thus the natural L-citrulline can be directly taken as a food additive and raw material of health products or medicines.

The invention discloses a high-yield arginine deiminase bacterial strain and a method for producing L-citrulline by the same. The high-yield arginine deiminase bacterial strain of the invention is streptococcus faecalis BM-2 CGMCC No.4990. After small-scale fermentation of the bacterial strain in a 30-L fermentation tank, the L-citrulline yield is up to 98 g / L, which is increased by 50% when compared with the L-citrulline yield of the original strain; after continuous fermentation for 5 batches, the L-citrulline yield of the bacterial strain is stable, and the L-citrulline yield level is obviously higher than that of reported bacterial strains; and the bacterial strain of the invention has important industrial production application value.

The invention discloses a mutated arginine deiminase as well as a preparation method and an application thereof. Amino acids in one or more sites of K137A, F198W, V230A, R257L and A260D of arginine deiminase mutate, and the mutated arginine deiminase is improved in activity significantly, can be used for catalyzing L-arginine to produce L-citrulline and can achieve the conversion rate of more than 95%. The mutated arginine deiminase is prepared by adopting an error-prone PCR (polymerase chain reaction) system.

Compositions for treating erectile dysfunction (“ED”), including age related erectile dysfunction (ARED), comprise in a preferred embodiment ginger and an amino acid. Preferably the amino acid is L-arginine and / or L-citrulline. Additionally, the compositions may comprise guarana (Paullinia cupana), Muira puama cortex magnoliae officinalis (CMO) and / or fructus aurantil immaturus (FAI). The compositions of the present inventions are administered in a pharmaceutically effective amount to treat erectile dysfunction. Essential ingredients in formulations of the present inventions for treatment of ED and ARED are ginger (or ginger derivative) and L-citrulline or L-arginine. A daily dosage of at least about 250 mg ginger and at least about 400 mg L-citrulline or L-arginine for a sufficient period of time will result in improved IIEF scores in a substantial number of patients.

L-citrulline may be extracted from watermelons using a process which does not require rigorous extraction with alcoholic and / or acidic solvents, or treatment at high temperatures. In the process, the watermelon juice is contacted with an adsorbent effective for adsorption of L-citrulline thereon, and the juice is separated therefrom. The L-citrulline on the adsorbent may then be eluted and recovered. Lycopene-containing microparticles may also be separated from the watermelon juice prior to contact with the adsorbent.

The invention relates to a composition having muscle power fatigue alleviating and anoxic tolerance improving effects. The composition is prepared by using L-arginine, L-citrulline, taurine, vitamin C, vitamin E powder and folic acid according to a prescribed ratio. Animal function tests prove that the composition can obviously prolong the weight bearing swimming time of mice, prolong the viability time of the mice under normal pressure anoxic conditions, obviously reduce the urea nitrogen and blood lactic acid content of serum of post-exercised mice, and increase the liver starch content of mice during exercise; and results of human feeding tests show that the composition can improve the maximum oxygen consumption of subjects, reduce the blood lactic acid rise after exercise and increase the exercise tolerance of bodies. The composition has muscle power fatigue alleviating and anoxic tolerance improving effects.

It is intended to provide a polypeptide having (i) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:1 by substitution of one or more amino acids in the amino acid sequences of from the 20- to the 38-th positions from the N end, or (ii) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:1 by substitution of one or more amino acids in the amino acid sequences of from the 20- to the 38-th positions from the N end and deletion, substitution or addition of one or more amino acids in the amino acid sequence of from the first to the 19-th positions or from the 39-th to the 294-th positions, and having an N-acetylglutamate kinase activity.

The invention provides a metabolic transformation strategy for efficiently improving production capacity of corynebacterium crenatum SYPA5-5 L-arginine, and belongs to the biological technical field. Histidine at a site 268 in N-acetyl glutamatekinase, NAGK, argB of SYPA-5 is mutated into asparaginate, so that feedback inhibition effect of the L-arginine on NAGK can be effectively relived; a mutation site is precisely introduced to a corresponding position of SYPA5-5 genome, so that yield of the obtained feedback inhibition-preventing bacterial strain (H-7)L-arginine is lowered, and metabolic intermediate (L-ornithine and L-citrulline) is largely accumulated. Transcriptional analysis is carried out on an arg gene cluster of h-7 to find that arg GH transcriptional level is lowered. Arg GH reinforced expression is carried out in H-7, and production capacity of the obtained recombinant bacterial strain H-7-GH, L-arginine is improved by 99.0% in comparison with SYPA5-5. Preliminary optimization is carried out on a fermentation culture medium of H-7-GH, so that yield of the L-arginine is 45.1g / L, which is improved by 49.8% in comparison with that before optimization, and improved by 1.13 times in comparison with SYPA5-5. Feedback inhibition of the L-arginine on NAGK is relieved, and metabolic transformation strategy of the arg GH gene is expressed in a strengthened manner at the same time, so that production capacity of the L-arginine of the SYPA 5-5 is effectively improved.

The invention discloses a method for extracting L-citrulline employing microbial fermentation of trichosanthes kirilowii maxim pulp, and belongs to the technical field of chemistry of traditional Chinese medicine. The method comprises the following steps: inoculating lactic acid bacteria to ferment; filtering; carrying out adsorption and exchange; concentrating; and crystallizing to obtain the L-citrulline. According to the method, by combination of fermentation method and an enzyme method, L-arginine in the trichosanthes kirilowii maxim pulp is converted into the L-citrulline under the action of deiminase which is generated by lactobacillus, so that the yield is greatly improved; no harmful solvent is added in the overall separation process; the obtained product is natural and safe; and development of health care products and development and research of medicines are facilitated.

The present invention relates to application of a novel signal peptide in L-arginine and its derivatives production from konjac powder, which belongs to the field of gene engineering, enzyme engineering and metabolism engineering. The present invention fused the signal peptide set forth in SEQ ID NO.1 with the β-mannanase of Bacillus subtilis CCTCC M 209200, and expressed the fused gene in the strain with high L-arginine yield. The recombinant strain Corynebacterium crenatum CGMCC 0890 / p MSPman had advantages on utilizing cheaper konjac powder as substrate, and after fermenting for 96 hours in a 5 L bioreactor, the L-arginine yield reached 45 g / L. Another two recombinant strains were constructed based on Corynebacterium crenatum CGMCC 0890 / pMSPman, and after fermenting for 96 hours in a 5 L bioreactor, the L-ornithine yield and L-citrulline reached 23.5 g / L and 26.3 g / L respectively.

The invention discloses an enhanced health product, which comprises the following raw materials by weight: 4-8 g of L-arginine, 80-150 mg of L-citrulline, 1-5 g of protein peptide, 100-400 mg of red rice extract, 100-300 mg of alpha-lipoic acid, 40-300 mg of coenzyme Q10 (water solubility), 20-80 mg of shellfish extract, 1-5 mg of folic acid, 80-250 mu g of organic selenium, 100-450 mg of soy isoflavone, 100-250 mg of V-C, 1-5 mg of seaweed extract, 20-100 mg of cinnamon bark extract, 40-140 mg of pumpkin seed extract, 50-300 mg of lemon extract, 10-50 mg of sealwort extract, and a traditional Chinese medicine preparation. The enhanced health product disclosed by the invention is capable of improving multiple symptoms.

The invention relates to a nitric oxide reinforcing agent and application thereof, belonging to the technical field of functional foods. The nitric oxide reinforcing agent is characterized by being prepared from the materials in the following proportion by weight: 3 to 10g of L-arginine, 0.1 to 2g of L-citrulline, 0.1 to 2mg of folacin, 20 to 800mg of tea polyphenol, 20 to 200mg of coenzyme Q 10, 0.4 to 3.2g of phytosterin and 2 to 20g of linseed meal. The invention can reinforce and stabilize NO produced within the body, and enhance the level of the NO in the body. In addition, the tea polyphenol, the folacin, the coenzyme Q 10, the phytosterin and the linseed meal have the antioxidation function in treatment and prevention of cardio-cerebrovascular diseases and respective unique physiological effects.

The invention provides pharmaceutical compositions having improved effects and synergistic efficacy against pain, and provides methods for their use to treat pain, wherein the composition comprises: a sulfur-containing amino acid; a carnitine compound; and at least one compound that is a L-citrulline compound or a beta-alanine compound.

The invention discloses a method for producing L-citrulline with high efficiency, and belongs to the technical field of bioengineering. The method comprises the steps of cloning in lactic acid bacteria by molecular biology methods to obtain peptidylarginine deiminase gene, improving the enzyme producing ability of ADI production strains by molecular biology, process optimization and other methods, and building a platform for producing L-citrulline by high-efficiency biological catalysis by optimizing a catalytic system. The bacteria obtained after fermentation can be directly used for conversation without being broken, the engineering bacteria have higher permeability at 50 degrees centigrade, so the absorption of substrate and release of products are promoted further, the reaction speed is improved greatly, the conversation period is only 4-12h, the yield of L-citrulline can reach 150-180g / L, and the conversation rate is greater than 94%.

The invention relates to the technical field of medicines, in particular to a targeting inducible nitric oxide synthase (iNOS) triptolide prodrug, and a preparation method and application thereof. R1of the triptolide targeting prodrug is L-arginine, L-arginine methyl ester, L-Nomega-NO-arginine, L-N omega-NO-arginine methyl ester, L-proline, L-citrulline, L-thiocitrulline or L-ornithine. The highexpression characteristic of the inducible nitric oxide synthase (iNOS) at an inflammatory site is utilized, and the triptolide is semi-synthesized by an iNOS specific substrate (group) to prepare the prodrug, the toxicity of the triptolide is reduced and the bioavailability are improved through the targeting property of the prodrug, the anti-inflammatory effect is significantly better than thatof the triptolide, and the triptolide can be effectively developed and utilized.

L-citrulline for treatment of endothelial dysfunction and in particular of erectile dysfunction and a pharmaceutical composition comprising L-citrulline in combination with one or more substances selected from the group consisting of pycnogenol, aqueous extract of Viscum album, NADPH, tetrahydrobiopterin, folic acid and mixtures thereof.

The invention discloses recombinant Escherichia coli for producing arginine deiminase at high yield. The recombinant Escherichia coli is collected with the serial number of CCTCC (China Center for Type Culture Collection) NO:M2015048 in CCTCC; an arginine deiminase gene is from Bacillus cereus which is bred after being subjected to induced mutation of N<+> ion beams; and then, a mutant strain for producing arginine deiminase at high yield is obtained through screening, wherein the collection number of the mutant strain is CCTCC NO:M2015047. The invention also discloses application of the recombinant Escherichia coli to production of L-citrulline and a method for producing L-citrulline. By using the recombinant Escherichia coli disclosed by the invention, more than 99% of arginine can be converted into L-citrulline, and the purity of a product can be up to more than 99%.

The invention discloses hyperfluorescence nitrogen doped carbon quantum dots and a preparing method and application thereof. The preparing method includes the steps that L-citrulline is dissolved in deionized water until the concentration is 0.010-0.040 g / mL, ultrasonic oscillation is carried out for 5-20 min, and a hydrothermal method is used for conducting reaction for 8-24 h at the temperature of 180-220 DEG C to obtain a carbon dot solution; the carbon dot solution is ultracentrifuged for 20-40 min at the speed of 17000-20000 r / min, and a 0.22 micrometer filter membrane is used for filtration to obtain the carbon dot solution. The invention further discloses application of the hyperfluorescence nitrogen doped carbon quantum dots in bean sprout and cervical cancer cells. The hyperfluorescence nitrogen doped carbon quantum dots are high in fluorescence efficiency, easy to prepare, low in toxicity, high in biocompatibility and water solubility and small in particle size.

The invention relates to an efficient liquid-phase detecting method of L-citrulline. The method is characterized in that the chromatographic conditions include that an amino bonded silica gel column is used as a chromatographic column; a mixed solution of 0.05mol / L of monopotassium phosphate buffering liquid and acetonitrile is used as a flowing phase, and the volume ratio of the phosphate buffering liquid to acetonitrile is 60: 30-40; the detection wavelength is 200-210nm; the column temperature is 25-35 DEG C; the flowing speed of the flowing phase is 0.7-1.0mL / min. With the adoption of themethod, the content of L-citrulline can be quickly and accurately detected; the error can be reduced; the quality is controlled; in addition, the operation is simple; the detection cost is low; the detection time is short; and the detection efficiency is high.

A clitoral sensitizing agent includes a base, L-citrulline, and a promotional vehicle.

The invention discloses a traditional Chinese medicinal health preparation. Each tablet of the preparation comprises 5mg of L-arginine (L-Arg), 19mg of L-citrulline, 8mg of folic acid, 16mg of vitamin C, 22mg of vitamin E, 17mg of alpha-thioctic acid, 25mg of olive extracts, 15mg of garlic extracts, 10mg of mannitol and 10mg of biological calcium. The preparation which contains no chemical preparations can be easily absorbed by human bodies and has the health functions of physique enhancement and immunity improvement.

The invention provides a function enhancer suitable for strenuous and speed-type physical activities and a preparation method thereof. The function enhancer is prepared from the following effective components: beta-alanine, L-arginine-alpha-ketoglutarate, creatine monohydrate, creatine citrate, L-citrulline-L-malic acid, zoysia tenuifolia seed extract, L-tyrosine, leucine, caffeine, taurine, olive leaf extract, black pepper extract, black carrot powder, vitamin complex and composite electrolyte. The function enhancer is prepared by a mixing mode. The function enhancer can enhance strength and explosive force in the process of physical activities, maximally stimulate the organism metabolism and relieve the feeling of fatigue, and ensure the organism reinforcing effect in training at the day after or in a competition state; and the preparation method of the function enhancer is simple and suitable for the requirement of large-scale industrial production.

The present invention relates to an herbal dietary supplement to promote vascular health. The dietary supplement comprises L-Arginine, L-Citrulline, Ginkgo Biloba, Horse chestnut, Red Yeast Rice and Cayanne Pepper.

The invention discloses an extraction method of L-citrulline in seed melon. The method comprises the steps of ultrafiltration liquid preparation, column elution, reduced-pressure distillation and crystallization. The invention also discloses an application of L-citrulline extracted with the above method. Specifically, materials with needed weight are prepared into acid particles and alkali particles; the particles are dried, and a lemon essence is sprayed in; the particles are dried, and are well mixed with polyethylene glycol 6000; and the mixture is tableted, such that L-citrulline effervescent tablets are obtained. The extraction method provided by the invention has the advantages of simple preparation process, low cost, low energy consumption, and low waste and pollutant production. The effervescent tablets prepared with the extracted L-citrulline have the advantages of fast disintegration, good effervescent effect, good sweet and sour taste, and convenient application. With the methods provided by the invention, a novel approach for comprehensively developing and utilizing seed melons is provided.

The invention relates to a strain MQO-153 for production of arginine deiminase. The strain MQO-153 has a preservation number of CGMCC No.10726, is preserved at China General Microbiological Culture Collection Center on April 21, 2015, and the preservation address is No.3, 1st yard, Beichen West Road, Chaoyang District, Beijing. The strain MQO-153 provided by the invention can be fermented to prepare arginine deiminase for production of L-citrulline by enzyme conversion method.

The invention discloses a supplementing composition for expanding the blood vessel and improving the explosive power and the endurance. The supplementing composition comprises L-citrulline, L-arginine, guarana extracts, creatine, apple polyphenol, polyphenol, tribulus terrestris extracts, beet root concentrated juice powder and the like. The supplementing composition has the effects of expanding the vessel, increasing the muscle blood flowing amount and improving the explosive power and the endurance of athletes and can meet the athletes' and fitness and bodybuilding people's requirements.