Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Strain MQO-153 for production of arginine deiminase

A technology of arginine deiminase and bacterial strains, which is applied in the direction of bacteria, hydrolase, and microorganism-based methods, can solve the problem of low activity of arginine deiminase, enzymatic methods are difficult to meet industrial production, and affect melon production. Eliminate the problems of citrulline production and other problems, achieve high product purity, overcome the bottleneck of citrulline production, and save the amount of consumption

Inactive Publication Date: 2015-12-23
SHANDONG MINQIANG BIOTECH
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (2) The difficulty of fermentation production is that the yield of L-citrulline per unit volume is low, the highest is only 1.7g / L, and the cost of extracting L-citrulline from the fermentation broth is relatively high
But its disadvantage is that the current enzymatic method is difficult to meet the requirements of industrial production. The reason is that the activity of arginine deiminase obtained from animal offal is low, the conversion rate is low, and the price of arginine raw materials is high, which affects Enzymatic production scale-up of citrulline

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Strain MQO-153 for production of arginine deiminase
  • Strain MQO-153 for production of arginine deiminase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 A bacterial strain MQO-153 producing arginine deiminase, the present invention through repeated chemical mutagenesis and ultraviolet mutagenesis to Streptococcus faecalis (Streptococcus faecalis purchased from Beijing Microorganism Culture Collection Center), And after screening a large number of strains, a strain MQO-153 with high arginine deiminase activity was obtained, and its preservation number is CGMCCNo.10726; the preservation unit is the General Microbiology Center of China Microbiological Culture Collection Management Committee ; The preservation date is April 21, 2015; the preservation address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

Embodiment 2

[0036] Example 2 The method for preparing arginine deiminase by using bacterial strain MQO-153, the specific steps are as follows:

[0037] (1) Slant culture: inoculate the bacterial strain MQO-153 on the slant medium under aseptic conditions for cultivation, the cultivation temperature is 30° C., and the cultivation time is 24 hours. At this time, the bacterial colony is large and smooth without pigment accumulation;

[0038] Slant medium: Slant medium (g / L): beef extract 5, peptone 10, sodium chloride 5, agar 20; pH is 7.0-7.3; preferably 7.2; the sterilization temperature of the slant medium is 115 ° C, sterilized The time is 15 minutes.

[0039] (2) Expansion culture: pick bacterium colony from the slant culture medium of step (1), add water and dilute to obtain thalline solution, the concentration after dilution is 3 * 10 5 -5×10 5 cells / mL, inoculate the bacterial cell solution onto the seed medium and place it in a shaker for expanded culture, the inoculum size is 10%...

Embodiment 3

[0044] Example 3 The method for preparing L-citrulline from L-arginine through the conversion of arginine deiminase, the specific steps are as follows:

[0045] (1) Obtaining the substrate: the arginine ceramic membrane filtrate was adsorbed by 732 ammonia resin, and eluted with 2.0N ammonia water to obtain arginine positive column purified solution;

[0046] (2) Transformation culture: the bacteria containing arginine deiminase were washed once with normal saline, and the bacteria were collected after centrifugation. Transfer the bacteria to the transformation solution, which includes 0.45mol / L acetate buffer and 0.5g / L CTAB, add 10% L-arginine purification solution, and incubate at 37°C for 24h to obtain L-citrulline Amino acid conversion solution.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a strain MQO-153 for production of arginine deiminase. The strain MQO-153 has a preservation number of CGMCC No.10726, is preserved at China General Microbiological Culture Collection Center on April 21, 2015, and the preservation address is No.3, 1st yard, Beichen West Road, Chaoyang District, Beijing. The strain MQO-153 provided by the invention can be fermented to prepare arginine deiminase for production of L-citrulline by enzyme conversion method.

Description

technical field [0001] The invention relates to a strain MQO-153 producing arginine deiminase, belonging to the field of biotechnology. Background technique [0002] In 1914, Uga Taro and others isolated citrulline for the first time from watermelon juice, and it was recognized as an amino acid by Wada Mitsutoku afterwards. Citrulline exists in the seeds of Cucurbitaceae plants in a free state. So far, people have successfully isolated citrulline H1 from watermelon juice, wild watermelon leaves, walnut kernels, walnut seedlings and seeds. Citrulline is a non-protein amino acid. Foreign scientists have done more research on citrulline, especially in Japan, the United States and Europe. In China, the research on citrulline is still in the stage of understanding, there is no corresponding report on the production method, and there are not many researches on its detection method and physiological function, only a small number of literature reports. Yue Rui et al. used dual-wav...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N9/78C12P13/10C12R1/46
Inventor 高法民高树营李令娣
Owner SHANDONG MINQIANG BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com