Preparation method and application of Japanese Raspberry root extract

A technology of tray root and extract is applied in the field of preparation of tray root extract, which can solve the problems of ineffective substances without therapeutic effect, affecting the application of liver protection, unclear effective parts, etc., and achieves wide indications, controllable quality and effective Well-defined effects

Inactive Publication Date: 2017-03-22
LIAONING INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in existing applications, tray preparations are only crushed and directly prepared into tablets, capsules, etc., or only decocted and concentrated, and then added with excipients to make preparations. The process is relatively simple and rough, and the effective parts are not clear. There are a large number of ineffective substances that have no therapeutic effect, taking a large amount, which may even lead to secondary liver damage, and its efficacy is greatly reduced, which seriously affects the application of its liver protection
[0008] In the prior art, the root extract of Tractoria root with total flavonoids and total triterpenoids as effective parts and its preparation method and process have not been involved. On the basis of the effective parts and pharmacological active ingredients of the damage protection effect, further research in depth and breadth, preparation of root extracts, full application of its effective parts and pharmacological active ingredients, removal of ineffective or even possible secondary liver damage ingredients, should be Has a good application prospect

Method used

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  • Preparation method and application of Japanese Raspberry root extract
  • Preparation method and application of Japanese Raspberry root extract
  • Preparation method and application of Japanese Raspberry root extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Step (1): Add 100g of tray root coarse powder, add 800ml of 70% ethanol to soak for 1h, reflux for extraction for 2h, filter, and collect the filtrate; add 600ml of 70% ethanol to the filter residue for reflux, extract for 1.5h, filter, and collect the filtrate; combine the filtrates, decompress Concentrate to a thick extract;

[0036] Step (2): Dissolve and dilute the extract with water at a ratio of 1:2, then add ethyl acetate at a volume ratio of 1:2 to extract three times, and concentrate the extracted part of ethyl acetate.

[0037] Step (3): In the water layer, evaporate the ethyl acetate, add n-butanol to extract 3 times at a volume ratio of 1:2, and concentrate the extracted part of n-butanol.

[0038] Step (4): Combine the extracts of (2) and (3), dilute with water to the equivalent of 5g of medicinal materials per 1mL, adsorb on D-101 macroporous adsorption resin, and elute with 10% ethanol for 3 times the volume of the column;

[0039] Step (5): Then use 50%...

Embodiment 2

[0041] Step (1): Add 100g of tray root coarse powder, add 800ml of water to soak for 0.5h, decoct for 1.5h, filter, and collect the filtrate; add 600ml of water to the filter residue, decoct for 1.0h, filter after decocting, and collect the filtrate; combine the filtrate, reduce Compress and concentrate to thick extract;

[0042] Step (2): Dissolve and dilute the tray root extract with water at a ratio of 1:1, then add ethyl acetate at a ratio of 1:3 for three times, and concentrate the extracted part of ethyl acetate.

[0043] Step (3): In the water layer, evaporate the ethyl acetate, add n-butanol to extract 3 times at a ratio of 1:3, and concentrate the extracted part of n-butanol.

[0044] Step (4): Combine the extracts of (2) and (3), dilute with water to the equivalent of 7g of medicinal materials per 1mL, absorb through AB-8 macroporous adsorption resin, and elute with 5% ethanol for 3 times the volume of the column;

[0045] Step (5): Then use 60% ethanol to elute 8 tim...

Embodiment 3

[0047] Step (1): 100g of tray root coarse powder, add 1000ml of 40% ethanol and soak for 1h, reflux extraction for 2.5h, filter, and collect the filtrate; Compress and concentrate to thick extract;

[0048] Step (2): Dissolve and dilute the tray root extract with water at a ratio of 1:3, then add ethyl acetate at a ratio of 1:1 to extract twice, and concentrate the extracted part of ethyl acetate.

[0049] Step (3): In the water layer, evaporate the ethyl acetate, add n-butanol to extract 3 times at a ratio of 1:1, and concentrate the extracted part of n-butanol.

[0050] Step (4): Combine (2) and (3), add water to dilute to the equivalent of 10g of medicinal materials per 1mL, absorb through HP-20 macroporous adsorption resin, and elute with 8% ethanol for 3 times the volume of the column;

[0051] Step (5): Then use 40% ethanol to elute 6 times of column volume, and the eluent is concentrated under reduced pressure and dried to obtain the root extract of Tractiolum, wherein...

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Abstract

The invention belongs to the technical field of researches and exploitation of medicine products, and provides a Japanese Raspberry root extract, and a preparation method and an application thereof. The Japanese Raspberry root is a medicinal composition containing 40-55 wt% of general flavones and 30-45 wt% of total triterpene, and the main active components of the extract are chemical components comprising kaempferol-3-O-beta-D-glucopyranoside, quercetin-3-O-beta-D-glucopyranoside and oleanolic acid. The extract has substantial improvement and protection effects on liver injuries, can be used for preparing medicines or health foods for prevention, treatment and auxiliary treatment of the liver injuries, and can be processed to form any present dosage forms, such as a tablet, a capsule, a powder injection, an injection, a pill, a granule and an oral liquid, preferable oral dosage forms, and more preferably the tablet, the capsule or the granule.

Description

[0001] 【Technical field】: [0002] The invention belongs to the technical field of research and development of pharmaceutical products. Specifically relates to the preparation and application of a tray root extract. [0003] 【Background technique】: [0004] Liver injury is a common liver disease, mainly caused by a series of damage caused by oxidative stress, which can easily evolve into hepatitis, liver fibrosis, liver necrosis, and even liver cirrhosis and liver cancer. Studies have shown that many traditional Chinese medicines and their active ingredients have obvious preventive and therapeutic effects on liver damage. Therefore, it is of theoretical and practical significance to search for and prepare extracts and preparations that can prevent and treat liver damage from natural medicines. [0005] Tray root is the root of Rubus crataegifolius Bunge, a plant of the family Rosaceae (Rosaceae), also known as burdock tripe, marling fruit, raspberry, tray, etc. It mainly grows...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P1/16A23L33/105A61K125/00
CPCA61K36/73A23V2002/00A61K2236/333A61K2236/39A61K2236/55A23V2200/32A23V2250/21
Inventor 黄占波宋冬梅楚冬海尚海巍葛会奇廉莲
Owner LIAONING INST OF SCI & TECH
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