Pseudomonas aeruginosa and microbial agent thereof, and application of both to passivation of lead

A technology of Pseudomonas aeruginosa and bacterial agents, applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms, can solve the problems of secondary pollution repair cycle, large engineering quantity, short repair cycle, etc., and achieve high economy Benefits and social benefits, easy operation, and the effect of improving dehydrogenase activity

Active Publication Date: 2017-03-22
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of large amount of work, easy to produce secondary pollution and long repair period in the existing methods for repairing heavy m

Method used

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  • Pseudomonas aeruginosa and microbial agent thereof, and application of both to passivation of lead
  • Pseudomonas aeruginosa and microbial agent thereof, and application of both to passivation of lead
  • Pseudomonas aeruginosa and microbial agent thereof, and application of both to passivation of lead

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042] Example 1

[0043] This example is used to illustrate the inactivation ability of Pseudomonas aeruginosa provided by the present invention to lead in water.

[0044] The bacterial solution of strain A prepared according to the above preparation example was inoculated into LB liquid medium with an inoculum of 1-2% by volume, and cultured with shaking at 160 rpm and 30°C for 10 hours, and then lead was added to the bacterial solution , Its initial concentration is 100mg / L. At 150-170 rpm, 28-30 ℃, vibration adsorption, sampling every 0.5h to determine the concentration of lead in the sample.

[0045] Among them, the detection method of lead content is: take an appropriate amount of sample, centrifuge at 8000 rpm for 10 min, take the supernatant, use ICP-AES to detect the lead content in the supernatant, and calculate the lead removal rate.

[0046] Lead removal rate (%) = (initial lead concentration-residual lead concentration) / initial lead concentration × 100%

[0047] The resul...

Example Embodiment

[0048] Example 2

[0049] This example is used to illustrate the inactivation ability of the bacterial agent provided by the present invention on lead in water.

[0050] The lead in the water body was passivated according to the method in Example 1, except that the freeze-dried bacterial cells of the strain A prepared according to the above preparation example were added.

[0051] The result is figure 1 Shown.

Example Embodiment

[0064] Example 3

[0065] This example is used to illustrate the promoting effect of Pseudomonas aeruginosa provided by the present invention on the microecology of lead-contaminated soil.

[0066] Add 100 mL of the bacterial liquid of strain A prepared according to the above preparation example to the lead-contaminated soil with a lead content of 100 mg / kg, stir evenly, and cultivate for 10 days to ensure that the water content is about 20-30%. After 10 days, dry the soil at 65°C, accurately weigh 1g into a 15mL centrifuge tube, add 2mL 0.1mol / L glucose solution and 2mL 0.5% TTC solution to the centrifuge tube, mix well and then at 37°C, 100rpm Incubate for 16h under dark conditions. Then, add 1 mL of formaldehyde to terminate the reaction, and then add 5 mL of acetone, and shake well to elute the product TPF attached to the soil particles. Finally, let it stand for 3 minutes, filter the supernatant with an organic filter membrane, and measure the absorbance of the filtrate at a...

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PUM

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Abstract

The invention specifically discloses Pseudomonas aeruginosa and application thereof. The accession number of the Pseudomonas aeruginosa is CGMCC No. 10844. The invention also discloses a microbial agent containing the Pseudomonas aeruginosa and a method for passivation of lead. The Pseudomonas aeruginosa provided by the invention has strong capability in passivation of lead in a water body, can finish passivation of lead within 4 to 5 h and has a maximal lead removal rate of 97.33%. As soil contaminated by lead is inoculated with the Pseudomonas aeruginosa, the dehydrogenase activity of the soil can be obviously improved, and lead can be effectively changed into a stable state from a transformable state so as to achieve the effect of lead passivation. Moreover, as the Pseudomonas aeruginosa is used for lead passivation, operation is simple, a period is short, secondary pollution is not posed and high economic benefits and social benefits are obtained.

Description

technical field [0001] The invention relates to a strain of Pseudomonas aeruginosa, in particular to a strain of Pseudomonas aeruginosa with the function of passivating lead and its bacterial agent, and their application in passivating lead. Background technique [0002] At present, the heavy metal pollution of domestic soil is very serious, 16% of the soil is poisoned by heavy metals to varying degrees, and tens of millions of tons of grain are lost every year, which seriously endangers production, life and human health. In recent years, lead poisoning incidents have occurred frequently, especially a series of incidents of children's blood lead exceeding the standard have aroused great concern and extensive discussion in China. Because lead exists widely in the environment, after entering the human body through the respiratory tract and digestive tract, it will cause a series of abnormalities in the nervous system, blood system and digestive system of the body, seriously af...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/385C02F101/20
Inventor 林森李晓桐任立伟王培红
Owner 粮华生物科技(北京)有限公司
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