Cotton verticillium wilt biocontrol bacillus spore producing fermentation medium and culture method and optimization method thereof

A technology for cotton verticillium wilt and fermentation medium, which is applied in the field of cotton verticillium wilt biocontrol bacillus spore-forming fermentation medium and its culture field, and can solve the problems of large influence on the growth of spores, complicated microbial fermentation process and the like. , to achieve the effect of simple method and lower production cost

Inactive Publication Date: 2017-03-22
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the production and application of biocontrol agents, the spore content of the strain is the key factor affecting its biocontrol effect. The fermentation process of microorganisms is very complicated. Great influence on growth and spore production

Method used

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  • Cotton verticillium wilt biocontrol bacillus spore producing fermentation medium and culture method and optimization method thereof
  • Cotton verticillium wilt biocontrol bacillus spore producing fermentation medium and culture method and optimization method thereof
  • Cotton verticillium wilt biocontrol bacillus spore producing fermentation medium and culture method and optimization method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The components and mass proportions of the spore-producing fermentation medium for cotton verticillium wilt biocontrol bacillus in this embodiment are: beef extract: 2.0%, corn flour: 2.0%, Na 2 HPO 4 : 0.4%, NaH 2 PO 4 : 0.2%, MgSO 4 ·7H 2 O: 0.05%, CaCl 2 : 0.02%, the rest is distilled water; the culture condition of this medium is: the speed is 180r min -1 , the culture temperature is 30°C, the culture time is 60h, the initial pH of the culture medium is 6.0, and the filling volume of the culture medium is 80mL / 200mL; 3% of the volume. The output of cotton Verticillium wilt biocontrol bacillus spores in the present embodiment is 4.8 * 10 8 CFU / mL.

Embodiment 2

[0050] The components and mass proportions of the spore-producing fermentation medium for cotton verticillium wilt biocontrol bacillus in this embodiment are: beef extract: 1%, maltose: 2%, Na 2 HPO 4 : 0.4%, NaH 2 PO 4 : 0.2%, MnSO 4 ·H 2 O, 0.05%, the rest is distilled water; the culture condition of this medium is: the rotating speed is 180r min -1 , the culture temperature is 30°C, the culture time is 84h, the initial pH of the culture medium is 7.0, and the liquid volume of the culture medium is 80mL / 200mL; 4% of the base volume. The output of cotton Verticillium wilt biocontrol bacillus spores in the present embodiment is 5.2 * 10 8 CFU / mL.

Embodiment 3

[0052] The components and mass proportions of the spore-producing fermentation medium for cotton verticillium wilt biocontrol bacillus in this embodiment are: soybean peptone: 1%, corn flour: 2%, Na 2 HPO 4 : 0.4%, NaH 2 PO 4 : 0.2%, CaCO 3 : 0.1%, the rest is distilled water; the culture condition of this medium is: the speed is 180r min -1 , the culture temperature is 30°C, the culture time is 70h, the initial pH of the culture medium is 8.0, and the liquid volume of the culture medium is 80mL / 200mL; 5% of the base volume. The output of cotton Verticillium wilt biocontrol bacillus spores in the present embodiment is 6.2 * 10 8 CFU / mL.

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Abstract

The invention discloses a cotton verticillium wilt biocontrol bacillus spore producing fermentation medium and a culture method and an optimization method thereof. The medium comprises, by mass, 1%-2% of a nitrogen source, 1%-2% of a carbon source, 0.2% of NaH2PO4.2H2O, 0.4% of Na2HPO4.2H2O, 0.02%-0.1% of other inorganic salts and the balance distilled water, wherein the other inorganic salts refer to at least one of CaCO3, K2HPO4 and MnSO4.H2O, and the pH is 6.0-8.0. The spore producing fermentation medium has advantages that cotton verticillium wilt biocontrol bacillus spore producing amount is increased, and spores of the strain can be well cultured; formulation of the spore producing fermentation medium is optimized through a single factor experiment, a Plackett-Burman experiment and a Box-Behnken experiment, spore producing culture conditions are optimized through an orthogonal experiment, production cost is reduced, and a technical foundation is established for industrial fermentation production of the strain.

Description

technical field [0001] The invention relates to a culture medium, in particular to a spore-producing fermentation medium for cotton Verticillium dahliae biocontrol bacillus, a culture method and an optimization method thereof. Background technique [0002] Cotton verticillium wilt in my country is mainly caused by Verticillium dahliae, a worldwide devastating seed-borne and soil-borne disease, commonly known as "cotton cancer". Verticillium dahliae has a wide range of hosts, reaching more than 600 species, mainly dicotyledonous plants such as Malvaceae, Fabaceae, Cruciferae and Solanaceae, and is susceptible to changes in the environment and hosts to produce new physiological types, and The formed micro-sclerotias survived in the soil for more than ten years, which caused certain difficulties in the control. At present, the prevention and control of cotton verticillium wilt at home and abroad mainly adopts methods such as crop rotation, breeding of disease-resistant varieti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/07
CPCC12N1/20
Inventor 李全胜谢宗铭张国丽武冬梅叶春秀赵曾强于航
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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