Bovine sperm capacitation solution and in vitro sperm capacitation method

A sperm capacitation and capacitation technology, applied in the field of livestock reproduction, can solve the problem of easy resistance to antibiotics and other problems, and achieve the effects of avoiding the problem of drug resistance, simple operation, and reducing production costs.
CN106520680AInactive Publication Date: 2017-03-22HENAN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN UNIV OF SCI & TECH
Publication Date
2017-03-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

Belonging to the technical field of livestock breeding, the invention discloses a bovine sperm capacitation solution and an in vitro sperm capacitation method. The bovine sperm capacitation solution mainly consists of the following components: 6.7500mg / mL NaCl, 0.3000mg / mL KCl, 2.5000mg / mL NaHCO3, 0.0620mg / mL NaH2PO4.2H2O, 0.2960mg / mL CaCl2.2H2O, 0.1000mg / mL MgCl2.6H2O, 0.1120mg / mL sodium pyruvate, 0.0186mg / mL EDTA-Na, 0.0100mg / mL phenol red, 6.0000mg / mL BSA, 1.0000mg / mL glucose, 0.4800mg / mL caffeine, 0.0500mg / mL heparin, and 0.025-0.05mg / mL Nisin. The capacitation solution provided by the invention can effectively ensure the sperm motility, sperm plasma membrane integrity rate, acrosomal integrity rate and mitochondrial activity, and maintains the in vitro sperm capacitation effect.
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Description

technical field

[0001] The invention relates to a bovine sperm capacitation solution, and also relates to a sperm capacitation method outside the body, belonging to the technical field of livestock reproduction. Background technique

[0002] With the development of artificial insemination and in vitro fertilization technology, the fertilization ability of bulls has become an important factor affecting the reproductive efficiency of cattle (Hoflack et al., 2006). Semen quality analysis is a common procedure for evaluating the fertility potential of bulls (Rijsselaere et al., 2002), so bovine semen quality analysis is particularly important (Hoflack et al., 2007). At the same time, the quality of semen is also closely related to the conception rate of cows, which is a key factor in improving the reproductive efficiency of cows. There are many factors that affect the quality of bull semen, such as age, nutrition, season and capacitation effect. The effective prerequisite to e...

Claims

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