Method for preparing pig cerebral protein antioxidative peptide

A technology of antioxidant peptide and brain protein powder, applied in the field of protein preparation

Inactive Publication Date: 2017-03-22
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The Cerebrolysin Injection manufactured by Austria Ebewe Pharmaceutical Factory (Ebewe) is the most widely used brain protein hydrolyzate product in clinical practice. It is 75% of free amino acids and 25% of small molecule peptides. Studies have shown that the biological activity of cerebrolysin is closely related to the small molecule peptides it contains. At present, there are few related studies on pig brain proteins and peptides, so the study of animal brain tissue The preparation method, separation, purification and biological activity of small molecular peptides in enzymatic hydrolyzate are of great significance to social development

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  • Method for preparing pig cerebral protein antioxidative peptide
  • Method for preparing pig cerebral protein antioxidative peptide
  • Method for preparing pig cerebral protein antioxidative peptide

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preparation example Construction

[0046] The invention provides a preparation method of porcine brain protein antioxidant peptide, which comprises the following steps:

[0047] Step 1, preprocessing:

[0048] Fresh pig brain was washed, chopped, degreased, dried and ground into powder to prepare defatted brain protein powder for later use;

[0049] Step 2. Ultrasonic treatment of pig brain protein powder:

[0050] Take the defatted cerebroprotein powder prepared in step 1, add deionized water according to the volume ratio of 1:200, stir magnetically for 20-40min, and place it in a water bath at 40-60°C for 0.5-1.5h to completely dissolve the cerebroprotein powder ;

[0051] Countercurrent pulse ultrasonic treatment: place the brain protein powder cooled to room temperature in a countercurrent pulse ultrasonic processor for processing, wherein the ultrasonic frequency is 20kHz, the power is 60-100W, ultrasonic 4-6min, the ultrasonic time during the period is 2s, and the intermittent time is 2s;

[0052] Step...

Embodiment 1

[0063] The preparation method of the pig brain protein antioxidant peptide comprises the following steps:

[0064] (1) Preparation of defatted pig brain protein powder

[0065] Fresh pig brain: purchased from Zhenjiang market, remove blood clots and meninges (pia mater and arachnoid), rinse with normal saline, chop, add 4 times the boiling water, boil for 5 minutes, filter and then add 75-90% of 2 times the volume Degrease with ethanol at 60°C for 20min, cool to room temperature, centrifuge at 3000-6000rmp for 10-20min, collect the precipitate, dry in an oven at 50°C to constant weight, grind, store in a desiccator, and store it for later use.

[0066] (2) Preparation of porcine brain polypeptide by ultrasonic-assisted enzymatic hydrolysis

[0067] Put 1g of defatted cerebroprotein powder in a 500mL Erlenmeyer flask, add 200mL of deionized water, stir magnetically for 20-40min, place it in a water bath at 40°C for 0.5h to completely dissolve the cerebroprotein powder, and coo...

Embodiment 2

[0075] The preparation method of the pig brain protein antioxidant peptide comprises the following steps:

[0076] (1) Preparation of defatted pig brain protein powder

[0077] Fresh pig brain: purchased from Zhenjiang market, remove blood clots and meninges (pia mater and arachnoid), rinse with normal saline, chop up, add 6 times the boiling water, boil for 15 minutes, filter and then add 75-90% of 4 times the volume Degrease with ethanol at 80°C for 20-40min, cool to room temperature, centrifuge at 3000-6000rmp for 10-20min, collect the precipitate, dry it in an oven at 50-70°C to constant weight, grind, store in a desiccator, and store it for later use .

[0078] (2) Preparation of porcine brain polypeptide by ultrasonic-assisted enzymatic hydrolysis

[0079] Put 2 g of defatted cerebroprotein powder in a 500 mL Erlenmeyer flask, add 400 mL of deionized water, stir magnetically for 40 minutes, place it in a water bath at 40-60 ° C for 1.5 hours to completely dissolve the ...

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Abstract

The invention discloses a method for preparing pig cerebral protein antioxidative peptide. The method comprises the following steps of 1, pretreatment, wherein fresh pig brain is taken to be prepared into degreased cerebral protein powder for use; 2, ultrasonic treatment of pig cerebral protein powder, wherein the degreased cerebral protein powder is added into deionized water according to the volume proportion of 1:200, the cerebral protein powder is completely dissolved; countercurrent pulse ultrasonic treatment is carried out, the cerebral protein powder cooled to room temperature is placed in a countercurrent pulse ultrasonic treating device to be treated, the ultrasonic frequency is 20 kHz, the power ranges from 60 W to 100 W, ultrasonic treatment is carried out for 4 min to 6 min, ultrasonic time is 2 s, and intermittent time is 2 s; 3, enzymolysis to extract crude pig brain polypeptide, wherein pig brain polypeptide crude extracting liquid is prepared; 4, separation, wherein an ultrafiltration membrane with the molecular cut off of 3-5 kDa is used for fractionation; 5, purifying, wherein a DEAE-52 fiber resin layer is utilized for carrying for secondary purification on the pig brain peptide prepared in the step 4, and the pig cerebral protein antioxidative peptide is prepared.

Description

technical field [0001] The invention relates to a protein preparation method. More specifically, the present invention relates to a preparation method of porcine brain protein antioxidant peptide. Background technique [0002] Every part of a pig has important nutritional value. In particular, pig brain is not only delicate, tender and delicious, rich in cephalin and protein, but also higher in calcium, phosphorus and iron than pork, which can increase the body's immunity, promote metabolism and brain function. [0003] Brain tissue is rich in nutrients and rich in protein. In recent years, researchers have isolated protein from animal brain tissue and enzymatically hydrolyzed it into a hydrolyzate containing free amino acids and low-molecular-weight peptides, using it to treat brain diseases. The Cerebrolysin Injection manufactured by Austria Ebewe Pharmaceutical Factory (Ebewe) is the most widely used brain protein hydrolyzate product in clinical practice. It is 75% of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/16
CPCC12P21/06C07K1/16C07K1/34
Inventor 邹烨王道营卞欢李鹏鹏蔡盼盼王立孙冲孙芝兰徐为民
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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