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Enzyme-labeled antibody diluent and preparation method thereof

A technology for enzyme-labeled antibodies and diluents, which is applied to measuring devices, instruments, scientific instruments, etc., can solve problems such as pollution and decreased titer of enzyme-labeled antibodies, and achieve the effect of preventing inactivation

Inactive Publication Date: 2017-03-22
河南省医药科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides an enzyme-labeled antibody application diluent and its preparation method, which solves the problem that the titer of the enzyme-labeled antibody will decrease after being diluted with the existing application diluent, and even cause pollution

Method used

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  • Enzyme-labeled antibody diluent and preparation method thereof
  • Enzyme-labeled antibody diluent and preparation method thereof
  • Enzyme-labeled antibody diluent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] An enzyme-labeled antibody application diluent is prepared by mixing basal solution and calf serum in a volume ratio of 3:1, and each liter of basal solution is prepared from the following components: 8.0 g of NaCl, 2.0 g of Na 2 HPO 4 ·12H 2 O, 0.25g of NaH 2 PO 4 ·2H 2 O, 0.1 g of KCl, 2.0 g of MgCl 2 ·2H 2 O, 10.8ml of 25% Tween-20, 36ml of glycerin, 6.75ml of 10g / 100ml of sodium sorbate, 2.7ml of 10g / 100ml of thimerosal, 4.0ml of gentamicin sulfate injection, 1.0g of gelatin, the remainder for distilled water. Prepare according to the following steps:

[0033] Step 1, prepare 25% Tween-20: use distilled water as a solvent to prepare Tween-20 with a volume fraction of 25%;

[0034] Preparation of 10g / 100ml sodium sorbate: use ethanol as a solvent to prepare sodium sorbate with a concentration of 10g / 100ml;

[0035] Preparation of 10g / 100ml thimerosal: use distilled water as solvent to prepare thimerosal with a concentration of 10g / 100ml;

[0036]Step 2, eac...

Embodiment 2

[0041] An enzyme-labeled antibody application diluent is prepared by mixing a basal solution and calf serum in a volume ratio of 3.5:1, and each liter of the basal solution is prepared from the following components: 8.5 g of NaCl, 2.0 g of Na 2 HPO 4 ·12H 2 O, 0.25g of NaH 2 PO 4 ·2H 2 O, 0.05g KCl, 1.5g MgCl 2 ·2H 2 O, 10ml of 25% Tween-20, 30ml of glycerol, 6ml of 10g / 100ml of sodium sorbate, 2ml of 10g / 100ml of thimerosal, 3.5ml of gentamicin sulfate injection, 1.0g of gelatin, and the remainder is distilled water. Prepare according to the following steps:

[0042] Step 1, prepare 25% Tween-20: use distilled water as a solvent to prepare Tween-20 with a volume fraction of 25%;

[0043] Preparation of 10g / 100ml sodium sorbate: use ethanol as a solvent to prepare sodium sorbate with a concentration of 10g / 100ml;

[0044] Preparation of 10g / 100ml thimerosal: use distilled water as solvent to prepare thimerosal with a concentration of 10g / 100ml;

[0045] Step 2, each l...

Embodiment 3

[0050] An enzyme-labeled antibody application diluent is prepared by mixing basal solution and calf serum in a volume ratio of 4:1, and each liter of basal solution is prepared from the following components: 9.0 g of NaCl, 2.0 g of Na 2 HPO 4 ·12H 2 O, 0.25g of NaH 2 PO 4 ·2H 2 O, 0.2g KCl, 2.5g MgCl 2 ·2H 2 O, 12ml of 25% Tween-20, 38ml of glycerin, 8ml of 10g / 100ml of sodium sorbate, 3ml of 10g / 100ml of thimerosal, 4.5ml of gentamicin sulfate injection, 1.2g of gelatin, and the balance is distilled water. Prepare according to the following steps:

[0051] Step 1, prepare 25% Tween-20: use distilled water as a solvent to prepare Tween-20 with a volume fraction of 25%;

[0052] Preparation of 10g / 100ml sodium sorbate: use ethanol as a solvent to prepare sodium sorbate with a concentration of 10g / 100ml;

[0053] Preparation of 10g / 100ml thimerosal: use distilled water as solvent to prepare thimerosal with a concentration of 10g / 100ml;

[0054] Step 2, each liter of bas...

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Abstract

The invention discloses an enzyme-labeled antibody diluent. The enzyme-labeled antibody diluent is formed by mixing a basic solution and calf serum in a volume ratio of 3-4:1, and each L of basic solution is prepared from 8.0-9.0 g of NaCl, 2.0 g of Na2HPO4-12H2O, 0.25g of NaH2PO4-2H2O, 0.05-0.20 g of KCl, 1.5-2.5 g of MgCl2-2H2O, 10-12 ml of 25% Tween-20, 30-38 ml of glycerol, 6-8 ml of 10 g / 100ml of sodium sorbate, 2-3 ml of 10 g / 100ml of thiomersalate, 3.5-4.5 ml of gentamicin sulphate injection, 0.8-1.2 g of gelatin and the balance distilled water. The enzyme-labeled antibody diluent is capable of preventing enzyme-labeled antibody inactivation and inhibiting growth of microorganisms such as bacteria and fungi. After being diluted by the concentrated enzyme-labeled antibody diluent, an enzyme-labeled antibody can be preserved for half a year at 2-8 DEG C.

Description

technical field [0001] The invention belongs to the technical field of biological reagents, and particularly relates to an enzyme-labeled antibody application diluent and a preparation method thereof. Background technique [0002] The prepared or purchased enzyme-labeled antibodies are generally stored frozen (-20°C) in a concentrated form, and then diluted several hundred to tens of thousands of times with diluents before being used in diagnostic reagents or experiments. The diluted enzyme-labeled antibody can only be used up within 1-3 days at 2-8°C, otherwise the enzyme-labeled antibody will not be reused due to its reduced titer and contamination. The stability of enzyme-labeled antibodies is a necessary condition for the preparation of diagnostic reagents and experimental applications. Therefore, it is necessary to study and prepare diluents that can keep enzyme-labeled antibodies stable in the application solution for a long time (6 months). And the laboratory provide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 杨小昂
Owner 河南省医药科学研究院