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Ginsenoside mixtures and application of mixtures serving as plant growth regulators

A technology of ginsenosides and mixtures, applied in the directions of plant growth regulators, plant growth regulators, applications, etc., can solve the problems of limited research and mining, and achieve the effects of enhancing rapid recovery, good research potential and application prospects, and enhancing resistance.

Active Publication Date: 2017-03-29
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, many scholars have reported the allelopathic and plant immune effects of plant secondary metabolites. These ingredients may have the potential to be developed into new plant growth regulators. Traditional Chinese medicine contains a large number of plant secondary metabolites. Research mining is very limited

Method used

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  • Ginsenoside mixtures and application of mixtures serving as plant growth regulators
  • Ginsenoside mixtures and application of mixtures serving as plant growth regulators
  • Ginsenoside mixtures and application of mixtures serving as plant growth regulators

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] Embodiment 1, the preparation of ginsenoside mixture

[0114] One, the preparation of ginsenoside mixture A

[0115] 1. Mix ginsenoside Rg1, ginsenoside Re, ginsenoside Rh2 and 20(S)-protopanaxatriol (PPT) in a mass ratio of 1:2:3:2 to obtain ginsenoside mixture A1.

[0116] 2. Mix ginsenoside Rg1, ginsenoside Re, ginsenoside Rh2 and 20(S)-protopanaxatriol (PPT) in a mass ratio of 2:4:3:1 to obtain ginsenoside mixture A2.

[0117] Two, the preparation of ginsenoside mixture B

[0118] 1. Mix ginsenoside Rb1, ginsenoside Rd, ginsenoside Rb3 and 20(S)-protopanaxadiol (PPD) in a mass ratio of 1:2:2:1 to obtain ginsenoside mixture B1.

[0119] 2. Mix ginsenoside Rb1, ginsenoside Rd, ginsenoside Rb3 and 20(S)-protopanaxadiol (PPD) in a mass ratio of 3:1:3:1 to obtain ginsenoside mixture B2.

Embodiment 2

[0120] Embodiment 2, the acquisition of ginseng adventitious root

[0121] 1, get ginseng seeds, soak 3min with 70% (volume percentage) ethanol aqueous solution, then soak 30min with 1% (volume percentage) sodium hypochlorite, wash three times with sterilized deionized water, embryoid body is transferred out from seed, and Culture in 1 / 2 MS solid medium C containing 1% (mass percentage) sucrose at 25±2°C for two weeks (16h light / 8h dark).

[0122] 2. Cut the root of the aseptic seedling cultivated in step 1 into a length of 1.0 cm, and use it as an explant for inducing adventitious root of ginseng. The explants contained 3.0mg·L -1 1 / 2MS of IBA (lack of NH 4 NO 3 ) in solid medium B at 25±2°C in dark culture, after 4 weeks of culture, adventitious roots formed on the explants, these adventitious roots were separated, and subcultured to cultures containing 3.0mg·L -1 1 / 2MS of IBA (lack of NH 4 NO 3 ) in liquid medium B at 25±2°C in the dark. Subculture once in about 3 we...

Embodiment 3

[0123] Example 3, the promoting effect of ginsenoside mixture A on the proliferation of ginseng adventitious root stem cells

[0124] 1. Inoculate the adventitious roots of ginseng obtained in Example 2 into liquid medium A containing ginsenoside mixture A1 prepared in Example 1 with different concentrations and cultivate them (the concentration of ginsenoside mixture A1 in the culture system is 0, 10, 50, 100 , 200μg / mL), the culture condition is 100r min -1 , (25±2)°C, cultured in dark for 3 days, and 3 parallel samples for each treatment concentration.

[0125] 2. Using Click- EdU Alexa The 647 Imaging Kit method was used to detect the proliferation of ginseng adventitious root stem cells treated in step 1. The adventitious roots of ginseng labeled with EdU and Hoechst33342 were observed by two-photon laser confocal microscope. The excitation light of EdU is 650nm, the emission light is 670nm, the excitation light of Hoechst 33342 is 350nm, and the emission light is ...

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Abstract

The invention discloses ginsenoside mixtures and application of the mixtures serving as plant growth regulators. The ginsenoside mixture A consists of ginsenoside Rg1, ginsenoside Re, ginsenoside Rh2 and 20(S)-protopanaxatriol, wherein the mass proportion of the ginsenoside Rg1 to the ginsenoside Re to the ginsenoside Rh2 to the 20(S)-protopanaxatriol is 1-2 to 2-4 to 3 to1-2. The ginsenoside mixture B consists of ginsenoside Rb1, ginsenoside Rd, ginsenoside Rb3 and 20(S)-protopanaxatriol, wherein the mass proportion of the ginsenoside Rb1 to the ginsenoside Rd to the ginsenoside Rb3 to the 20(S)-protopanaxatriol is 1-3 to 1-2 to 2-3 to 1. The ginsenoside mixtures serve as the plant growth regulators and have good research potential and application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a ginsenoside mixture and its application as a plant growth regulator. Background technique [0002] Plant growth regulators are a type of species that regulate and control plant metabolism during a certain period of plant growth and development and under certain environmental conditions. They play an important role in enhancing plant resistance, increasing production and income, and improving quality. . Traditional plant growth regulators mainly refer to artificially synthesized plant hormones. In recent years, more and more attention has been paid to the green production of crops and food safety issues, and the chronic toxicity of traditional plant growth regulators in the application of pesticides, fertilizers, herbicides, etc. has also received more and more attention. The development and utilization of plant growth regulators is imminent. [0003] In recent years, many schola...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N45/00A01P21/00
CPCA01N45/00
Inventor 黄璐琦袁媛刘娟纪瑞锋张迎春陈同
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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