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A kind of preparation method of artificial regeneration bone

An artificial and preparation process technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of trauma application and difficult treatment at the donor site, and achieve the goal of reducing lactate dehydrogenase activity, improving exercise ability, and promoting regeneration. Effect

Active Publication Date: 2019-12-10
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Trauma, tumors, chronic inflammation and other diseases can cause bone defects in the human body. For larger bone defects, substitute implantation is required in clinical treatment. At present, the implants used in clinical practice are mainly autologous bone, and autologous bone transplantation is usually used for repair. Small-area bone defects are considered by the industry to be a safe and reliable method with a high rate of bone fusion, but the disadvantages of postoperative trauma to the donor site and limited amount of bone harvest limit its application in large-area bone defects
Therefore, it is still a big problem to clinically treat patients with large bone defects exceeding 30%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment one: a kind of preparation method of artificial regeneration bone, its specific preparation process is:

[0021] (1), get fresh bone from the corpse of the person who just passed away, and remove blood stains and periosteum, then wash away the bone marrow in the fresh bone with PBS solution, then soak the fresh bone in PBS solution to remove blood stains, etc. Impurities are cleaned;

[0022] (2), put the above-mentioned clean bone into alcohol with a volume concentration of 75% and soak for 2 times. Soak and shake in PBS solution of Triton X-100, wherein: the mass concentration of Triton X-100 in PBS solution is 1%, and the volume ratio of bone block to Triton X-100 / PBS mixed solution is 1: 3. Change the medium every 6 hours, and change the medium 4 times in total, then remove the bone block from the Triton X-100 / PBS mixed solution, and rinse it twice with PBS solution;

[0023] (3), the bone block is soaked in the PBS solution containing pancreatic lipase...

Embodiment 2

[0030] Embodiment two: a kind of preparation method of artificial regeneration bone, its specific preparation process is:

[0031] (1), get fresh bone from the dead rabbit's body, remove blood stains and periosteum, then wash away the bone marrow in the fresh bone with PBS solution, then soak the fresh bone in PBS solution to remove blood stains, etc. Impurities are cleaned;

[0032] (2) Put the above-mentioned clean bone into alcohol with a volume concentration of 75% for soaking once, each soaking time is 1min, then rinse the bone twice with PBS solution, and then immerse the bone in a solution containing koji Soak and shake in the PBS solution of Triton X-100, wherein: the mass concentration of Triton X-100 in the PBS solution is 3%, and the volume ratio of the bone block to the Triton X-100 / PBS mixed solution is 1: 1. Change the medium once every 8 hours, and change the medium twice in total, then remove the bone block from the Triton X-100 / PBS mixed solution, and rinse i...

Embodiment 3

[0040] Embodiment three: a kind of preparation method of artificial regeneration bone, its specific preparation process is:

[0041] (1), get fresh bone from the dead pig's corpse, and remove blood stains and periosteum, then wash away the bone marrow in the fresh bone with PBS solution, then put the fresh bone into PBS solution to soak blood stains, etc. Impurities are cleaned;

[0042] (2), put the above-mentioned clean bone into alcohol with a volume concentration of 75% and soak for 2 times, each soaking time is 2min, then rinse the bone with PBS solution for 3 times, and then immerse the bone in a solution containing koji Soak and shake in the PBS solution of Triton X-100, wherein: the mass concentration of Triton X-100 in the PBS solution is 2%, and the volume ratio of the bone block to Triton X-100 / PBS mixed solution is 1: 2. Change the medium every 7 hours, for a total of 3 times, then remove the bone fragment from the Triton X-100 / PBS mixed solution and rinse it once...

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Abstract

The invention discloses a preparation method of an artificial regenerative bone. The preparation method is characterized by specifically comprising the steps of fresh bone block decellularization treatment, gel preparation, concentrated marrow preparation and artificial regenerative bone preparation. The preparation method has the advantages that the artificial regenerative bone obtained by processing through the preparation method can be used for curing large segmental bone defect of a human body or an animal body and is safe, reliable and high in bone fusion rate.

Description

technical field [0001] The invention relates to the technical field of tissue engineering bionics, in particular to a method for preparing artificially regenerated bone. Background technique [0002] Trauma, tumors, chronic inflammation and other diseases can cause bone defects in the human body. For larger bone defects, substitute implantation is required in clinical treatment. At present, the implants used in clinical practice are mainly autologous bone, and autologous bone transplantation is usually used for repair. Small-area bone defects are considered by the industry to be a safe and reliable method with a high rate of bone fusion, but the disadvantages of trauma to the donor site and limited amount of bone harvest limit its application in large-area bone defects. Therefore, it is still a big problem to clinically treat patients with large bone defects exceeding 30%. Contents of the invention [0003] The technical problem to be solved by the present invention is to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/54A61L27/52
CPCA61L27/20A61L27/3608A61L27/365A61L27/3687A61L27/52A61L27/54A61L2300/232A61L2300/404A61L2430/02A61L2430/40C08L5/00
Inventor 竺亚斌
Owner NINGBO UNIV
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