Check patentability & draft patents in minutes with Patsnap Eureka AI!

A method and kit for detecting the genotype of cyp2c19*3 polymorphic site

A technology of CYP2C19 and polymorphic sites, applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., to achieve the effect of improving accuracy

Active Publication Date: 2021-01-05
GUANGDONG PHARMA UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, PCR-RFLP involves enzyme digestion, and the traditional experimental design without internal control enzyme digestion sites will hardly avoid misjudgment of results in the case of incomplete enzyme digestion

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method and kit for detecting the genotype of cyp2c19*3 polymorphic site
  • A method and kit for detecting the genotype of cyp2c19*3 polymorphic site
  • A method and kit for detecting the genotype of cyp2c19*3 polymorphic site

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] In this example, 3 pairs of primers for detecting the genotype of the CYP2C19*3 polymorphic site were designed for the restriction endonuclease HinfI, and a detection method for the genotype of the CYP2C19*3 polymorphic site was established, as follows:

[0091] 1. Genomic DNA extraction

[0092] Using oral swab genomic DNA extraction kit or blood genomic DNA extraction kit, extract genomic DNA according to the operation steps in the manual. The DNA source is not limited thereto, and any somatic cell-derived DNA is acceptable.

[0093] 2. Primer design

[0094] Get the 1000bp sequence before and after the CYP2C19*3 polymorphic site, analyze its homology with other genes of the CYP2C subfamily, namely CYP2C9, CYP2C18, and CYP2C8, and use Primer Premier 5.0 software to design specific typing for this site primers. Among them, the internal control enzyme cutting site is GATTC upstream of the polymorphic site or GAGTC downstream of the polymorphic site, see image 3 , ...

Embodiment 2

[0144] This embodiment is aimed at the restriction endonucleases EcoRI, MvaI [the isozymes include AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI, EcoR II, BciT130I, etc.], Bme1390I [the isozymes include ScrFI, BmrFI, BssKI, BstSCI, MspR9I, StyD4I, etc.], BseDI [isozymes include BsaJI, BssECI, etc.] designed a pair of primers for detecting the CYP2C19*3 polymorphic site genotype, and established the CYP2C19*3 polymorphic site genotype The detection method is as follows:

[0145] 1. Genomic DNA extraction

[0146] Using oral swab genomic DNA extraction kit or blood genomic DNA extraction kit, extract genomic DNA according to the operation steps in the manual. The DNA source is not limited thereto, and any somatic cell-derived DNA is acceptable.

[0147] 2. Primer design

[0148] Get the 1000bp sequence before and after the CYP2C19*3 polymorphic site, analyze its homology with other genes of the CYP2C subfamily, namely CYP2C9, CYP2C18, and CYP2C8, and use Primer Premier 5.0 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting a CYP2C19*3 polymorphic site genetype and a kit. The method comprises the steps of introducing a specific 'inner quality control' mechanism during a process of detecting the CYP2C19*3 polymorphic site genetype, i.e. introducing an internal control restriction enzyme cutting site in a PCR (Polymerase Chain Reaction) product, identifying a situation of incomplete enzyme cutting according to a macrorestriction map, and still accurately judging the genetype under the situation of incomplete enzyme cutting, so that the accuracy of a detection result is greatly improved.

Description

technical field [0001] The invention belongs to the field of molecular detection, and in particular relates to a method and a kit for detecting the genotype of a CYP2C19*3 polymorphic site. Background technique [0002] CYP2C19 is an important member of the cytochrome P450 (CytochromeP450, CYP) enzyme system and an important drug metabolizing enzyme in the body. It was first isolated from human liver by Wrighton et al. in 1993. The full length of its cDNA is 1940bp, and the coding region is 1473bp , encoding 490 amino acid residues. CYP2C19 participates in a wide range of drug metabolism, including proton pump inhibitors (omeprazole, lansoprazole, pantoprazole), antidepressants (fluoxetine, citalopram, escitalopram, albino Mitriptyline, clomipramine, imipramine, moclobemide, trimipramine, etizolam), antiepileptic drugs (valium, clobazam, phenytoin, phenobarbital, valproic acid ), platelet aggregation inhibitors (clopidogrel), antimalarials (proguanil), antifungals (voricon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6876C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6876C12Q2600/156C12Q2521/301C12Q2525/131C12Q2531/113
Inventor 何震宇谢小妃陈宝荣钟子源陈珂蕊
Owner GUANGDONG PHARMA UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com