Method for dry preservation of biological tissue

Abstract

The invention relates to a method for dry preservation of biological tissue. The method for dry preservation of the biological tissue comprises the steps that three solutions containing organic solvents and glycerol are made to be in contact with the biological tissue in sequence, a part of the solutions are removed from the biological tissue treated through the solutions, and dry preservation is conducted after sterilization and chilling. The size of the biological tissue treated through the method is basically identical with the size of the biological tissue which is not dehydrated, the side effect of water is completely eliminated, the dried tissue is not prone to being influenced by microorganisms, and therefore medical staff can obtain the biological tissue which approaches to the ready-to-use state to the greatest extent.

Description

technical field [0001] The invention relates to the field of implant materials, in particular to a method for dry preservation of biological tissues. Background technique [0002] As a kind of organic material, biological tissue material has become a research hotspot because of its good hemodynamic performance, less risk of embolism, less hemolysis, no need for anticoagulant therapy, convenient material acquisition, and low cost. But its main disadvantage is that it is more prone to tissue degeneration, calcification and rejection, which affects its durability. [0003] Many scholars have engaged in a lot of research, trying to overcome these defects. It was not until 1968, when French scholars Carpentier et al. proposed the use of glutaraldehyde to treat and preserve biological tissues, that the durability of biological tissues was greatly improved, and biological tissues treated with glutaraldehyde were widely used clinically again. [0004] However, long-term clinical p...

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Problems solved by technology

[0004] However, long-term clinical practice has proved that the biological tissues treated with glutaraldehyde cross-linking have the following defects: 1) cytotoxicity, the long-term residual glutaraldehyde in the cross-linked tissue has toxic effects on cells. 2) The existence of aldehyde groups and other reasons lead to calcification of the tissue after cross-linking, which is one of the important reasons for the decline of biological tissues; 3) Incomplete elimination of immunogenicity is one of the reasons for calcification and also leads to inflammatory reactions One of the mechanisms; 4) Residual cells and debris are one of the main sites of tissue calcification and an important source of immunogenicity; 5) Tissues with residual reagents must be washed before implantation

[0006] CN101626682A discloses a biological tissue for surgical implantation and a processing method thereof, the method for processing the biological tissue includes making the biological tissue with polyhydric alcohol and C 1 -C 3 non-aqueous treatment solution of alcohol, so that the treated biological tissue will return to at least about 97% of its original hydrated size, while avoiding the disadvantages of glutaraldehyde treatment and preservation of biological tissue; however, the processing time required by this method is relatively long. Long, the amount of solution required is large, which is not conducive to simplifying operations and reducing costs

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