Method for dry preservation of biological tissue

A biological tissue and drying technology, applied in the field of implant materials, can solve the problems of easy calcification of tissues, cytotoxicity, long processing time, etc., and achieve the effect of eliminating side effects and high degree of proximity

Inactive Publication Date: 2017-04-19
上海纽脉太惟医疗科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] However, long-term clinical practice has proved that the biological tissues treated with glutaraldehyde cross-linking have the following defects: 1) cytotoxicity, the long-term residual glutaraldehyde in the cross-linked tissue has toxic effects on cells. 2) The existence of aldehyde groups and other reasons lead to calcification of the tissue after cross-linking, which is one of the important reasons for the decline of biological tissues; 3) Incomplete elimination of immunogenicity is one of the reasons for calcification and also leads to inflammatory reactions One of the mechanisms; 4) Residual cells and debris are one of the main sites of tissue calcification and an important source of immunogenicity; 5) Tiss

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0048] The glutaraldehyde-treated biological tissue (porcine pericardium was used in this experiment) was cut into three groups of similar size, 6 pieces in each group. Measure the size and record it. The pig pericardium was completely submerged in solution A for 12 hours, completely submerged in solution B for 12 hours after taking it out, and completely submerged in solution C for 12 hours after taking it out. Wherein solution A, B, the volume ratio of C and pig pericardium are all 80:1, and its distribution ratio of each group is as shown in Table 1.

[0049] Table 1

[0050] Solution combination Solution A Solution B Solution C glycerin 75％ 80％ 85％ ethanol 25％ 20％ 15％

[0051] Test items:

[0052] 1. Measure and record the diameter and thickness of the pericardial slices before treatment, after drying and after hydration, and read after the data is stable for 5 seconds. The same pericardial slice was tested 6 times at different positi...

Embodiment 2-7

[0074] The components shown in Table 9 are used to prepare solutions A, B and C, and the order of solutions A→B→C is used to contact biological tissues. After testing, the diameter and thickness changes before treatment, after drying and after hydration The rates are shown in Table 10.

[0075] Table 9

[0076]

[0077] Table 10

[0078]

[0079] It can be seen from the above results that in Examples 1-7, the solutions A, B and C are used to contact the biological tissue in the order of solution A→B→C, which can make the size of the treated biological tissue closer to the size before dehydration. , so that the dry tissue is less susceptible to microorganisms; in the embodiments 1-7, compared with the other embodiments 2-7, the embodiment 1 can make the size of the biological tissue after treatment the closest to the size before dehydration.

[0080] The present invention has also verified other animal pericardium (such as cattle, sheep, kangaroos, etc.), aorta, mitral ...

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Abstract

The invention relates to a method for dry preservation of biological tissue. The method for dry preservation of the biological tissue comprises the steps that three solutions containing organic solvents and glycerol are made to be in contact with the biological tissue in sequence, a part of the solutions are removed from the biological tissue treated through the solutions, and dry preservation is conducted after sterilization and chilling. The size of the biological tissue treated through the method is basically identical with the size of the biological tissue which is not dehydrated, the side effect of water is completely eliminated, the dried tissue is not prone to being influenced by microorganisms, and therefore medical staff can obtain the biological tissue which approaches to the ready-to-use state to the greatest extent.

Description

technical field [0001] The invention relates to the field of implant materials, in particular to a method for dry preservation of biological tissues. Background technique [0002] As a kind of organic material, biological tissue material has become a research hotspot because of its good hemodynamic performance, less risk of embolism, less hemolysis, no need for anticoagulant therapy, convenient material acquisition, and low cost. But its main disadvantage is that it is more prone to tissue degeneration, calcification and rejection, which affects its durability. [0003] Many scholars have engaged in a lot of research, trying to overcome these defects. It was not until 1968, when French scholars Carpentier et al. proposed the use of glutaraldehyde to treat and preserve biological tissues, that the durability of biological tissues was greatly improved, and biological tissues treated with glutaraldehyde were widely used clinically again. [0004] However, long-term clinical p...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 虞奇峰梁玉晨常丽南秦涛
Owner 上海纽脉太惟医疗科技有限公司
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