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Method for selecting and breeding variety with diversified resistance by molecular marker-assisted selection

A molecular marker-assisted and diverse technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of long breeding cycle, rapid loss of resistance of bred varieties, low utilization rate of germplasm resources, etc. question

Inactive Publication Date: 2017-04-19
HUAIYIN TEACHERS COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current conventional strategies for breeding and applying rice disease-resistant varieties have defects and problems such as long breeding cycle, low utilization rate of germplasm resources, and rapid loss of resistance in bred varieties.

Method used

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  • Method for selecting and breeding variety with diversified resistance by molecular marker-assisted selection
  • Method for selecting and breeding variety with diversified resistance by molecular marker-assisted selection
  • Method for selecting and breeding variety with diversified resistance by molecular marker-assisted selection

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Identification and screening of crossing, backcrossing and selfing populations between the resistant parent Huazhan containing rice blast resistance gene Pi2 and the parent 9311 to be improved:

[0041] The rice material Huazhan containing the rice blast resistance gene Pi2 was used to cross the high-quality, high-yielding medium indica variety 9311, which is resistant to rice blast and bacterial blight, to obtain F1 generation materials, which were selected by Pi2-InDel at the seedling stage. Positive individual plants were removed, and negative individual plants were pulled out; F1 generation materials were identified for rice blast resistance gene Pi2 at the seedling stage, and individual plants containing the target gene were retained; field phenotypic traits were investigated from the seedling stage, and then agronomic traits were screened out. For the same individual plants, take the mixed pollen of these individual plants and backcross with 9311 at the suitable hybr...

Embodiment 2

[0060] Identification and screening of hybrid, backcross and self-cross populations between resistant parent 75-1-127 containing rice blast resistance gene Pi9 and parent 9311 to be improved:

[0061] Using the rice material Huazhan containing the rice blast resistance gene Pi9 to cross with the high-quality, high-yield medium indica variety 9311, which is resistant to rice blast and bacterial blight to be improved, F 1 Identification of rice blast resistance gene Pi9 at the seedling stage was carried out from the generation materials, and the single plants containing the target gene were retained, and then the single plants with the same agronomic traits as 9311 were screened out, and the mixed pollen of these single plants was backcrossed with 9311 at a suitable hybridization period. BC 1 f 1 Progeny materials were also marked at the seedling stage to select positive individual plants, screen out individual plants with the same agronomic traits as 9311, and take the mixed p...

Embodiment 3

[0081] Identification and screening of crossing, backcrossing and selfing populations between the resistant parent CBB23 containing the bacterial blight resistance gene Xa23 and the parent 9311 to be improved:

[0082] The rice material CBB23 containing the bacterial blight resistance gene Xa23 was crossed with the high-quality, high-yield medium indica variety 9311, which is resistant to rice blast and bacterial blight. For the identification of the sex gene Xa23, keep the individual plants containing the target gene, and then screen out the individual plants with the same agronomic characteristics as 9311, and take the mixed pollen of these individual plants to backcross with 9311 at the appropriate hybridization period. The BC1F1 generation materials were also marked at the seedling stage to select positive individual plants, and the individual plants with the same agronomic traits as 9311 were screened out, and the mixed pollen of these individual plants was backcrossed wit...

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Abstract

The invention provides a method for selecting and breeding a new rice variety resistant to Pyricularia oryzae and Xanthomonas oryzae, and belongs to the technical field of rice disease resistance breeding. The method comprises the steps of selecting and using a two-line restoring line 9311 with a maximum promotion area in production as a to-be-improved parent, and performing hybridization with a Pyricularia oryzae and Xanthomonas oryzae resistant parent, backcrossing and selfing by utilizing a method of combining molecular marker-assisted selection with field agronomic selection; performing resistance identification on an obtained target gene homozygous line, and selecting and breeding three lines with constant agronomic traits and improved target resistance traits; and finally mixing the three lines by different ratios according to actual conditions of different planting areas to obtain the new variety with the diversified resistance, namely, "a super-resistant variety 9311". According to the method, the Pyricularia oryzae and Xanthomonas oryzae resistance of the rice variety with the diversified resistance is hugely improved on the basis of ensuring agronomic traits of an original background material, namely, the production increment and stabilization are facilitated, and the environmental protection is facilitated; and the method is of great significance for ensuring grain sustainable development.

Description

technical field [0001] The invention belongs to the technical field of rice disease-resistant breeding, and specifically relates to a method for assisting breeding of resistant and diverse varieties by using molecular markers. Background technique [0002] According to existing research reports, rice blast caused by Pyricutaria oryzae Cav. and bacterial blight caused by Xanthomonas campestris pv. oryzae are the most serious fungi to rice production in the world. STDs and Bacterial Diseases. These two diseases have brought serious harm to the stable and increased production of rice. In general epidemic years, rice production can be reduced by about 20%, and in severe cases, it may even result in a complete failure of the harvest. In recent years, with the narrowing of the genetic basis of hybrid rice parents and the unreasonable use of nitrogen fertilizers in cultivation, rice blast and bacterial blight have been increasing year by year, posing a great threat to rice product...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A01H1/02A01H1/04
CPCA01H1/02A01H1/04C12Q1/686C12Q1/6895C12Q2531/113C12Q2565/125
Inventor 杨立明纪剑辉周颖君罗玉明方继朝刘永锋
Owner HUAIYIN TEACHERS COLLEGE
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