Kidney yin deficiency syndrome animal model biomarker screening and determining method

Abstract

The invention provides a kidney yin deficiency syndrome animal model biomarker screening and determining method. The method comprises the following steps: extracting a cortical bone total protein sample, measuring the protein concentration, carrying out bidirectional gel electrophoresis analysis, detecting the gel protein spot, displaying and analyzing differential protein spots, carrying out MALDI-TOF-MS of differential protein spots, searching the protein database to identify and determine differential proteins, identifying part of differential proteins through Western-Bolt and RT-PCR, and interactively verifying the identification results with ELISA detection results of differential proteins in the serum. The differential proteins, whose expression trends are consistent and are not in the normal value range, can be used as the biomarker for identifying a kidney yin deficiency syndrome animal model. The biomarker can be used as the discrimination index of kidney yin deficiency syndrome in animal experiments; and a base is provided for finding another kidney yin deficiency syndrome biomarker.

Description

technical field [0001] The invention belongs to the field of basic research, and in particular relates to a method for screening and determining biomarkers of animal models of kidney yin deficiency. Background technique [0002] "Syndrome" is the core of the theoretical system of traditional Chinese medicine. The characteristic of traditional Chinese medicine in treating diseases lies in syndrome differentiation and treatment, but syndrome differentiation is largely affected by doctors themselves (such as understanding of traditional Chinese medicine knowledge, clinical experience, etc.) Objectification and quantification lead to inconsistencies in the results of syndrome differentiation, affect the establishment of treatment methods, the selection of prescriptions and medications, and the evaluation of clinical efficacy, hindering the research and development of traditional Chinese medicine. The use of animal models in basic research of traditional Chinese medicine can simul...

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Problems solved by technology

[0002] "Syndrome" is the core of the theoretical system of traditional Chinese medicine. The characteristic of traditional Chinese medicine in treating diseases lies in syndrome differentiation and treatment, but syndrome differentiation is largely affected by the doctor himself (such as understanding of traditional Chinese medicine knowledge, clinical experience, etc.) Objectification and quantification lead to inconsistencies in the results of syndrome differentiation, affect the establishment of treatment methods, the selection of prescriptions and medications, and the evaluation of clinical efficacy, hindering the research and development of traditional Chinese medicine. The use of animal models in basic research of traditional Chinese medicine can simulate the reality of diseases to the greatest extent. The process is similar to the physiological and pathological state of "syndrome", so the use of animal models of kidney yin deficiency syndrome is the only way to study the essence of kidney deficiency syndrome

In this experiment, the symptoms, signs, serum cAMP, cGMP content detection and cAMP / cGMP ratio results of rats with kidney yin deficiency caused by gavage of thyroid tablet suspension were in line with the identification requirements of the commonly used kidney yin deficiency model. It shows that the animal model of kidney yin deficiency syndrome in this study was successfully constructed, but the above evaluation methods are mainly subjective evaluation, and the evaluation indicators lack specificity and objectivity. Can the research results scientifically clarify the essence of TCM "syndrome" and the replication Whether the animal syndrome model is representative also involves the objectification and quantification of syndrome

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