Detection method of lactic acid in baijiu base liquor

A technology of lactic acid detection and detection method, which is applied in the field of detection of lactic acid in liquor-based liquor, can solve the problems of low accuracy and cumbersome detection process of lactic acid, and achieve the effect of high accuracy and wide detection concentration range

Active Publication Date: 2017-04-19
COFCO GROUP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the cumbersome and low-accuracy detection process of lactic acid in liquor in the prior art, the present invention conducts in-depth research, and provides a simple and easy-to-operate, high-accuracy detection method by using high-performance liquid chromatography (HPLC) for detection. Determination method of lactic acid in liquor base liquor

Method used

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  • Detection method of lactic acid in baijiu base liquor
  • Detection method of lactic acid in baijiu base liquor
  • Detection method of lactic acid in baijiu base liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Chromatographic conditions:

[0043] Chromatographic column: Bio-Rad HPX-87H (with sulfodiethylene and benzene-styrene copolymer as stationary phase), 300*7.8mm; pre-column: Bio-Rad 125-0129, 30*4.6mm;

[0044] Mobile phase: 0.005mol / L H 2 SO 4 ;

[0045] Flow rate: 0.6mL / min;

[0046] Column temperature: 65°C;

[0047] Injection volume: 15μL;

[0048] Running time: 30min;

[0049] Detector: differential refractive index detector, detector temperature 50°C.

[0050] Detection steps:

[0051]Measure 50mL of Luzhou-flavor base wine sample and place it in a 100mL rotary evaporator. Use a rotary evaporator to evaporate to dryness at a rotary evaporating temperature of 38°C and a rotary evaporating pressure of 1200Pa. Add 15mL of 15% vol ethanol solution to fully dissolve the sample and shake well, and then filter with a 0.22 μm filter membrane to obtain the sample solution to be tested for use.

[0052] Take the above-mentioned lactic acid standard product and the ...

Embodiment 3

[0054] Test equipment and conditions

[0055] Chromatographic column: Bio-Rad HPX-87H (with sulfodiethylene and benzene-styrene copolymer as stationary phase), 300*7.8mm; pre-column: Bio-Rad 125-0129, 30*4.6mm;

[0056] Mobile phase: 0.005mol / LH 2 SO 4 ;

[0057] Flow rate: 1.0mL / min;

[0058] Column temperature: 65°C;

[0059] Injection volume: 15μL;

[0060] Running time: 30min;

[0061] Detector: differential refractive index detector, detector temperature 50°C.

[0062] Detection steps:

[0063] Measure 50mL of Luzhou-flavor base wine sample, put it in a 100mL rotary evaporator, and use a rotary evaporator to evaporate to dryness at a rotary evaporation temperature of 38°C and a rotary evaporation pressure of 1200Pa, and add 15mL of 15% vol ethanol solution to make the sample fully Dissolve and shake well, and then filter with a 0.22um filter membrane to obtain the sample solution to be tested for use.

[0064] Take the above-mentioned lactic acid standard product...

Embodiment 4

[0068] The accuracy analysis of embodiment 4 detection method of the present invention

[0069] A certain amount of lactic acid standard sample is added to 15% vol ethanol solution to prepare a sample with known lactic acid content, which is tested according to the method of the present invention. The measured lactic acid concentration was compared to the known concentration to obtain the recovery. The results of the accuracy analysis are listed in Table 1 below. Through accuracy analysis, it can be seen that the average recovery rate of lactic acid in the method of the present invention is 99.77%.

[0070] Table 1 Accuracy analysis results

[0071]

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Abstract

The invention provides a detection method of lactic acid in baijiu base liquor. The detection method comprises following steps: low temperature reduced pressure distillation is adopted for complete evaporation of a baijiu base liquor sample; a low concentration ethanol aqueous solution is added for redissolving of the evaporated sample; and then high performance liquid chromatography detection is carried out. According to the detection method, a simple pretreatment method is adopted; a high performance liquid chromatograph is used for direct sample injection; the content of lactic acid in baijiu base liquor can be obtained accurately; the detection method is rapid, is high in accuracy, and wide in detection concentration range; influences of compounds such as esters, other organic acids, and higher alcohols in baijiu base liquor can be eliminated; and the detection method is of important significance in scientific guidance on baijiu base liquor production.

Description

technical field [0001] The invention relates to a method for detecting lactic acid in liquor-based liquor, which belongs to the technical field of food. Background technique [0002] Lactic acid is the main substance that constitutes the flavor of liquor and has a certain masking effect on alcohol. In liquor, lactic acid can act as a buffer to reconcile the taste of the wine, and can be compatible with various components to coordinate the body of the wine and reduce the potency. There is a certain correspondence between the content of lactic acid in liquor and the quality of liquor. For example, when the lactic acid content in liquor is low, the taste of the liquor can often be adjusted, and there is a refreshing feeling after drinking. When the content of lactic acid is appropriate, it can generally give the liquor a good flavor, mellow and rich. However, when the lactic acid content is too high, the liquor will have a sour and astringent taste, which will adversely affe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 刘劲松刘辉何志远姜开荣张圣龙从志会郭福阳金明亮宫殿良王泽兴张俊奇沈乃东张宏嘉车夏宁唐堂
Owner COFCO GROUP
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