Amphipathic octenyl succinic short-straight chain starch nano-particles and preparation method thereof

A technology of octenyl succinic acid and octenyl succinic anhydride, which is applied in the field of nanoparticles and its preparation process, can solve the problems of hydrophobic drug embedding, low degree of substitution, large particles, etc., to protect the active structure and improve the resistance Oxidation activity, effect of small size

Active Publication Date: 2017-04-26
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the starch ester obtained by this method has amphiphilic properties, the formed particles are large and have a low degree of substitution. They can only be used as thickeners and surfactants, and cannot be used as carriers to embed hydrophobic drugs.

Method used

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  • Amphipathic octenyl succinic short-straight chain starch nano-particles and preparation method thereof
  • Amphipathic octenyl succinic short-straight chain starch nano-particles and preparation method thereof
  • Amphipathic octenyl succinic short-straight chain starch nano-particles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Prepare octenyl succinic acid short amylose nanoparticles according to the following steps:

[0055] Preparation of short amylose: first select common corn starch, prepare 5mg / mL gelatinized starch milk with 1M phosphate buffer solution of pH 4, add pullulanase with a final concentration of 15U / mL to it, and enzymatically hydrolyze at 45°C After 12 hours, a short amylose solution was obtained. The short amylose was precipitated with absolute ethanol and washed three times with water, and the precipitate was freeze-dried at 8 Pa and -80° C. for 50 hours to obtain short amylose dry powder.

[0056] Preparation of short amylose amylose amylose amylose with different modification degrees: first, prepare the short amylose dry powder obtained in the above step into a 50 mg / mL aqueous solution, and put it in an oil bath at 121°C for 30 minutes, then cool down to 55 ℃, and use 0.1M NaOH to adjust the pH of short amylose to 8, then add the octenyl succinic anhydride solution (o...

Embodiment 2

[0059] Prepare octenyl succinic acid short amylose nanoparticles according to the following steps:

[0060] Preparation of short amylose starch: firstly select waxy corn starch, prepare 10 mg / mL gelatinized starch milk with 1 M phosphate buffer solution of pH 4.5, and add pullulanase with a final concentration of 10 U / mL to it, 55 ℃ enzymolysis for 8 hours to obtain a short amylose solution. The short amylose was precipitated with absolute ethanol and washed three times with water, and the precipitate was freeze-dried at 10 Pa and -70° C. for 60 hours to obtain short amylose dry powder.

[0061] Preparation of short amylose amylose amylose amylose with different modification degrees: firstly prepare the short amylose dry powder obtained in the above step into a 150mg / mL aqueous solution, and put it in an oil bath at 135°C for 30min, then cool down to 50 ℃, and use 0.1M NaOH to adjust the pH of short amylose to 8.5, then add the octenyl succinic anhydride solution (octenyl Th...

Embodiment 3

[0064] Prepare octenyl succinic acid short amylose nanoparticles according to the following steps:

[0065] Preparation of short amylose starch: first select waxy rice starch, prepare 3 mg / mL gelatinized starch milk with 1 M phosphate buffer solution of pH 5, and add pullulanase with a final concentration of 5 U / mL to it, and enzyme at 55 °C Decompose for 6 hours, obtain short amylose solution. The short amylose was precipitated with absolute ethanol and washed three times with water, and the precipitate was freeze-dried at 5 Pa and -60° C. for 72 hours to obtain short amylose dry powder.

[0066] Preparation of short amylose amylose amylose amylose with different modification degrees: first, prepare the short amylose dry powder obtained in the above step into a 100 mg / mL aqueous solution, and put it in an oil bath at 150°C for 20 minutes, then cool down to 52 ℃, and use the NaOH of 0.1M to adjust the pH of short amylose to 9, then add the octenyl succinic anhydride solution ...

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Abstract

The invention relates to preparation process of amphipathic octenyl succinic short-straight chain starch nano-particles. The preparation process comprises the following steps: (1) performing enzymolysis on gelatinized starch by using pullulanase, and obtaining short straight chain starch; (2) preparing a short straight chain starch solution, performing gelatinization, adding an octenyl succinic anhydride solution which accounts for 25-100% of the weight of dry powder of short straight chain starch, and continuously stirring for 6-10 hours, so as to obtain a modified octenyl succinic short straight chain starch solution; preparing a 1-10mg / mL solution of the octenyl succinic short-straight chain starch, stirring for 6-10 hours at 37-40 DEG C, and cooling to the room temperature, thereby obtaining a amphipathic octenyl succinic short-straight chain starch nano-particle solution. The particle size of the nano-particles is within 5-100nm, the nano-particles are good in tissue adhesion property, are capable of wrapping hydrophobic active substances, are high in loading rate and low in cost, the conveying efficiency of lyophobic active components by gastrointestinal tracts can be improved, and the bioavailability of the nano-particles can be improved. The lyophobic active components can be protected, the stability of the lyophobic active components can be improved, and the release of adverse flavor can be shielded; in addition, the addition amount and the toxic and side effects of bioactive components can be effectively reduced.

Description

technical field [0001] The invention relates to the fields of preparation of nanoparticles and the fields of food and medicine, in particular to nanoparticles obtained by modifying short amylose by octenyl succinic anhydride and a preparation process thereof. Background technique [0002] In recent years, due to the ability of amphiphilic polymer materials to self-assemble into nanostructures with different functions, more and more attention has been paid to them, especially natural biopolymer nanocarriers, which are more popular because they are non-toxic and harmless to the human body. . However, most of the current amphiphilic polymer nanoparticles are chemically synthesized, which are not very suitable for embedding human drugs, and there are few reports on amphiphilic polymers modified by natural polymers. [0003] Starch is an important class of natural polysaccharide polymer materials with biodegradability, regeneration and good biocompatibility. It has abundant sour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K9/127A61K47/36A61K9/51A61K31/047
CPCA61K9/0002A61K9/1075A61K9/1273A61K9/5161A61K31/047A61K47/36
Inventor 孙庆杰熊柳常然然杨洁葛胜菊李芳卢浩刘青
Owner QINGDAO AGRI UNIV
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