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Method for extracting organic acids from fruits

An organic acid and fruit technology, applied in the field of natural product extraction, can solve the problems of complex fruit and fruit components, difficult control of enzymatic-assisted extraction conditions, interference of organic acid determination, etc., and achieves rapid extraction, high accuracy, and reproducibility. good effect

Inactive Publication Date: 2017-04-26
JIANGSU JINGWEI TECH INNOVATION CONSULTING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex components of fruits, sugar, pigments, ethanol, polyphenols and other components may interfere with the determination of organic acids; in addition, methanol and ether used in the extraction of organic acids have certain toxicity, and the conditions for enzymatic assisted extraction are not easy. control, therefore, it is necessary to establish a stable extraction method suitable for the analysis of organic acids in fruit fruits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for extracting organic acids from fruits, comprising the steps of:

[0019] Step 1, in parts by weight, take 100 parts of strawberry homogenate, add 150 parts of water and 6 parts of cellulase, carry out enzymolysis, inactivate the enzyme, and obtain the enzymolysis solution;

[0020] Step 2, filtering the enzymolysis solution through a sand filter device to obtain a filtrate;

[0021] Step 3, evaporating the filtrate until the volume is reduced to 40%, adding an equal volume of ethanol aqueous solution for extraction, and taking the organic phase;

[0022] Step 4, adding an equal volume of 1wt.% sodium hydroxide aqueous solution to the organic phase, stirring evenly, and taking the water phase;

[0023] Step 5, sending the water phase into an ultrafiltration membrane for filtration, and concentrating the filtrate under reduced pressure to obtain the final product.

[0024] Wherein, in step 1, the enzymolysis temperature is 50°C, and the enzymolysis time is 4...

Embodiment 2

[0027] A method for extracting organic acids from fruits, comprising the steps of:

[0028] Step 1, in parts by weight, take 100 parts of grape homogenate, add 180 parts of water and 8 parts of cellulase, carry out enzymolysis, inactivate the enzyme, and obtain the enzymolysis solution;

[0029] Step 2, filtering the enzymolysis solution through a sand filter device to obtain a filtrate;

[0030] Step 3, evaporating the filtrate until the volume is reduced to 45%, adding an equal volume of aqueous ethanol for extraction, and taking the organic phase;

[0031] Step 4, adding an equal volume of 1.5wt.% sodium hydroxide aqueous solution to the organic phase, stirring evenly, and taking the water phase;

[0032] Step 5, sending the water phase into an ultrafiltration membrane for filtration, and concentrating the filtrate under reduced pressure to obtain the final product.

[0033] Wherein, in step 1, the enzymolysis temperature is 52°C, and the enzymolysis time is 3h; in step 3...

Embodiment 3

[0036] A method for extracting organic acids from fruits, comprising the steps of:

[0037] Step 1, in parts by weight, take 100 parts of apple homogenate, add 180 parts of water and 9 parts of cellulase, carry out enzymolysis, inactivate the enzyme, and obtain the enzymolysis solution;

[0038] Step 2, filtering the enzymolysis solution through a sand filter device to obtain a filtrate;

[0039] Step 3: Evaporate the filtrate until the volume is reduced to 48%, add an equal volume of aqueous ethanol for extraction, and take the organic phase;

[0040] Step 4, adding an equal volume of 2wt.% sodium hydroxide aqueous solution to the organic phase, stirring evenly, and taking the water phase;

[0041] Step 5, sending the water phase into an ultrafiltration membrane for filtration, and concentrating the filtrate under reduced pressure to obtain the final product.

[0042] Wherein, in step 1, the enzymolysis temperature is 55°C, and the enzymolysis time is 3h; in step 3, the con...

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Abstract

The invention provides a method for extracting organic acids from fruits. The method comprises the following steps: homogenizing the fruits, adding water and cellulase, performing enzymatic hydrolysis, performing enzyme deactivation, filtering a enzymatic hydrolysate by a sand filter device, then evaporating a filtrate till the volume is reduced to 40-50%, adding an equal volume of aqueous ethanol solution for extracting, and taking out an organic phase; adding an equal volume of 1-3wt.% aqueous sodium hydroxide solution into the organic phase, stirring uniformly, finally sending a water phase into an ultrafiltration membrane for filtering, and performing reduced pressure concentration on a filtrate to obtain the organic acids. Various organic acid components extracted by the extraction method provided by the invention are high in precision and accuracy and good in reproducibility; the extraction method is fast, simple and convenient, and is suitable for determining the contents of the main organic acid components in the fruits.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting organic acids from fruits. Background technique [0002] Organic acid generally refers to an acidic organic compound containing a carboxyl group (-COOH) in its molecular structure. A considerable part of the dry matter in fruit is organic acid, which often determines the special taste of fruit more than many other substances, and occupies an important position in food nutrition. The composition and content of organic acids are important components of fruit quality and flavor. Organic acids accumulated in plants also participate in photosynthesis, respiration, and the synthesis of amino acids, phenols, esters, and aromatic substances. Efficient extraction methods for major organic acids are of great significance. [0003] The types and contents of organic acids in fruits are the result of the combined effects of internal gen...

Claims

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Application Information

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IPC IPC(8): C07C51/42C07C51/43C07C51/48C07C59/265C07C59/255C07C59/245C07C55/10
CPCC07C51/42C07C51/43C07C51/48
Inventor 吴娟
Owner JIANGSU JINGWEI TECH INNOVATION CONSULTING CO LTD
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