Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Daptomycin purifying method

A purification method, daptomycin technology, applied in the field of antibiotic purification, can solve the problems of cumbersome operation, harsh control conditions, expensive equipment, etc., and achieve the effect of simple operation and low cost

Active Publication Date: 2017-04-26
JIANGSU HENGRUI MEDICINE CO LTD +1
View PDF14 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has disadvantages such as cumbersome operation, harsh control conditions, expensive packing and expensive equipment.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] After cooling 300L of the daptomycin fermentation broth, clarify it with a ceramic membrane with a membrane pore size of 0.1-0.2μm, discard the bacteria residue, and collect 890L of the clarified liquid.

[0039] 890L of the clarified liquid was purified by a 50L HP20 resin column, washed with 33% methanol containing 30mM sodium acetate at pH 5.5 and eluted with 50% methanol containing 30mM sodium acetate at pH 6.5 in sequence to obtain Daptomycin with a purity > 92% 90L of prime elution collection solution.

[0040] 90L of the collected solution was diluted with purified water to contain 10% methanol, purified through a 50L HP20SS resin column, washed with 5% methanol containing 30mM sodium acetate at pH 5.5 and eluted with 45% methanol containing 30mM sodium acetate at pH 6.5, 80 L of daptomycin elution collection solution with a purity >94% was obtained.

[0041] 80 L of collected solution was purified by FPDA-13 ion exchange resin column, the organic solvent was re...

Embodiment 2

[0046] After cooling 280L of the daptomycin fermentation broth, clarify it with a plate frame, discard the bacteria residue, and collect 250L of the clarified liquid.

[0047]Purify 250L of the clarified liquid through a 50L HP20 resin column, wash with 35% methanol containing 40mM sodium acetate at pH 5.0 and elute with 55% methanol containing 30mM sodium acetate at pH 6.0 in sequence to obtain Daptomyces with a purity > 92% 85L of prime elution collection solution.

[0048] 85L of the collected solution was diluted with purified water to contain 10% isopropanol, purified through a 50L HP20SS resin column, washed with 7% methanol containing 30mM sodium acetate at pH 5.0 and washed with 48% methanol containing 30mM sodium acetate at pH 6.0 80 L of collected daptomycin solution with a purity >94% was obtained.

[0049] 80L of collected solution was purified by 40L FPDA-13 ion exchange resin column, washed with 40mM sodium chloride and 30mM sodium acetate, eluted with 400mM sod...

Embodiment 3

[0054] After cooling 320L of the daptomycin fermentation broth, clarify it with a ceramic membrane with a membrane pore size of 0.1-0.2 μm, discard the bacteria residue, and collect 960L of the clarified liquid.

[0055] The clarified liquid was purified through a 50L HP20 resin column, washed with 20% isopropanol containing 30mM sodium acetate at pH 5.5 and eluted with 32.5% isopropanol at pH 6.5 containing 30mM sodium acetate in sequence to obtain a purity > 92%. Daptomycin elution collection solution 100L.

[0056] The collected solution was diluted with purified water to contain 10% isopropanol, purified through a 40L HP20SS resin column, washed with 25% isopropanol containing 45mM sodium acetate at pH 5.5 and 40% isopropanol containing 35mM sodium acetate at pH 6.5 Elute with propanol to obtain 90 L of collected elution solution of daptomycin with a purity >94%.

[0057] The collected solution was purified by 40L FPDA-13 ion exchange resin column, washed with 35mM sodium...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention provides a daptomycin purifying method. Specifically, the method comprises the steps that firstly, fermentation liquor is clarified and then is separated by macro-porous adsorption resin and anion exchange resin, then nanofiltration concentration is adopted to remove endotoxin, and desalination, concentration, filtration and the like are performed to obtain high-purity daptomycin, wherein macro-porous adsorption resin separation and anion exchange resin separation can be exchanged in step sequence. The product obtained by the adoption of the daptomycin purifying method is high in purity and contains few impurities, and operation is simple.

Description

technical field [0001] The invention relates to a method for purifying antibiotics, in particular to a method for purifying daptomycin. Background technique [0002] Daptomycin is a new class of cyclolipopeptide antibiotics, which is fermented by Streptomyces roseosporus. It is the first cyclolipopeptide antibiotic successfully developed in the world. Daptomycin has a novel structure and a unique bactericidal mechanism. It can destroy the function of bacterial cell membranes in many aspects and kill Gram-positive bacteria. effective for a long time. It is clinically used to treat infections caused by aerobic Gram-positive bacteria. Daptomycin remains effective against antibiotic-resistant Gram-positive bacteria, including those resistant to methicillin, vancomycin, and linezolid. [0003] In 2003, the FDA approved daptomycin injection for the treatment of complicated skin and soft tissue infections caused by Gram-positive bacteria, as well as bacteremia and right-sided en...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C07K1/36C07K1/34C07K1/22C07K1/18
CPCC07K7/08
Inventor 陈舟舟湛滢王宏伟彭洁韩培沈灵佳
Owner JIANGSU HENGRUI MEDICINE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com