Polypeptide-modifying polyamide-amine branch type polymer and preparation method and application thereof

A technology of polypeptide modification and polyamide, which is applied in the field of biomedical antibacterial materials, can solve the problems of differences in antibacterial properties and cytotoxicity, complicated preparation and application, etc., and achieves the effects of firm adsorption, simple preparation process, and easy industrial production.

Active Publication Date: 2017-04-26
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current drug immobilization system mainly immobilizes antibacterial substances on the surface of implants through chemical covalent bonds. The design of different covalent bonds is different, and the preparation and application are more complicated

Method used

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  • Polypeptide-modifying polyamide-amine branch type polymer and preparation method and application thereof
  • Polypeptide-modifying polyamide-amine branch type polymer and preparation method and application thereof
  • Polypeptide-modifying polyamide-amine branch type polymer and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] In this example, the synthetic route of the 4th generation polyamide-amine type dendritic polymer whose terminal group is modified by polypeptide is as follows: figure 1 As shown, the specific preparation method is as follows:

[0050] (1) Synthesis of polyamide-amine dendrimers whose end groups are acryloyl groups

[0051] ① Prepare the first solution

[0052] At room temperature and normal pressure, 1.0 g of the polyamide-amine dendrimers (G4PAMAM-NH 2 ) was dissolved in 1 mL of anhydrous dimethyl sulfoxide to form the first solution, in which G4PAMAM-NH 2 The concentration is about 1g / mL;

[0053] ② Prepare the second solution

[0054] Dissolve triethylamine (TEA) in the above-mentioned first solution at room temperature and normal pressure to form a second solution; the TEA and the G4PAMAM-NH in the first solution 2 The molar ratio of molecules is 3:1;

[0055] ③Synthesis

[0056] Under the protection of argon, add acryloyl chloride (Acryloyl chloride) to the...

Embodiment 2

[0069] In this example, the synthetic route of the 5th generation polyamide-amine dendrimers whose terminal groups are modified by polypeptides is as follows: figure 1 As shown, the specific preparation method is as follows:

[0070] (1) Synthesis of polyamide-amine dendrimers whose end groups are acryloyl groups

[0071] ① Prepare the first solution

[0072] At room temperature and normal pressure, 2.5g of the polyamide-amine dendrimers (G5PAMAM-NH 2 ) was dissolved in 2mL of anhydrous dimethyl sulfoxide to form the first solution, in which G5PAMAM-NH 2 The concentration is about 1.25g / mL;

[0073] ② Prepare the second solution

[0074] Dissolve triethylamine (TEA) in the above-mentioned first solution at room temperature and normal pressure to form a second solution; the TEA and the G5PAMAM-NH in the first solution 2 The molar ratio of molecules is 3.3:1;

[0075] ③Synthesis

[0076] Under the protection of argon, add acryloyl chloride (Acryloyl chloride) to the secon...

Embodiment 3

[0089] In this example, the synthetic route of the 6th generation polyamide-amine dendrimers whose end groups are modified by polypeptides is as follows: figure 1 As shown, the specific preparation method is as follows:

[0090] (1) Synthesis of polyamide-amine dendrimers whose end groups are acryloyl groups

[0091] ① Prepare the first solution

[0092] At room temperature and normal pressure, the polyamide-amine dendrimers (G6PAMAM-NH 2 ) was dissolved in 3mL of anhydrous dimethyl sulfoxide to form the first solution, in which G6PAMAM-NH 2 The concentration is about 1.33g / mL;

[0093] ② Prepare the second solution

[0094] Dissolve triethylamine (TEA) in the above-mentioned first solution at room temperature and normal pressure to form a second solution; the TEA and the G6PAMAM-NH in the first solution 2 The molar ratio of molecules is 3.4:1;

[0095] ③Synthesis

[0096]Under the protection of argon, add acryloyl chloride (Acryloyl chloride) to the second solution at ...

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Abstract

The invention provides a polypeptide-modifying polyamide-amine branch type polymer, which has a structural formula shown as the accompanying drawing. In the structural formula, n is the number of the polyamide-amine branch type polymer with a terminal group being amino group, and the n is 4, 5 or 6; m is the grafted polypeptide number, and the m is 3 to 4. The invention also provides a preparation method of the polypeptide-modifying polyamide-amine branch type polymer, and proves that the polypeptide-modifying polyamide-amine branch type polymer can be firmly adsorbed on the surface of hydroxylapatite, and has an antibacterial effect; the polypeptide-modifying polyamide-amine branch type polymer can be used as a human body implant body hydroxylapatite surface long-effect antibacterial coating material.

Description

technical field [0001] The invention belongs to the field of biomedical antibacterial materials, in particular to polyamide-amine dendrimers modified by polypeptides and their preparation methods and applications. Background technique [0002] Implant infection due to bacterial colonization is one of the important factors leading to implant failure, and the need for treatment of such complications is growing rapidly. Hydroxyapatite (HA) is widely used as artificial bone or loaded on the surface of implants because of its good biocompatibility and good combination with bone tissue, so as to improve the relationship between artificial bone or implants and human bone. The bonding of tissues increases the stability of the implant. Therefore, constructing an antibacterial coating on the HA surface of implants to inhibit bacterial colonization is an important means to prevent implant infection. At present, antibacterial coating drug release system and drug immobilization system ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G73/02A61L27/34A61L27/12
CPCA61L27/12A61L27/34A61L2420/02A61L2430/02A61L2430/12C08G73/028C08L79/02
Inventor 李建树苟雅萍杨潇李继遥
Owner SICHUAN UNIV
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