A method for extracting ferulic acid from bran
A technology for ferulic acid and bran, which is applied in the field of extracting ferulic acid from bran by using high-pressure puffing pretreatment technology, can solve the problems of low extraction rate, difficult to fully release ferulic acid and the like, and achieves improved extraction rate, Improve water retention and enhance the effect of wall-breaking
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Embodiment 1
[0030] The brewer's grain bran collected from the brewery was dried in a drying oven at 70°C; the dried bran was crushed to a particle size of about 0.3mm with a grinder; 2g of the sieved bran was weighed and sterilized The pot is sterilized for 30 minutes to inactivate its endogenous enzyme activity. Add 40 mL of 0.1N sodium hydroxide and soak at 60°C for 12 hours; adjust the pH of the soaked bran to 7.0 with acetic acid, and cut it for 30 minutes with a high-speed shearing machine at 18,000 r / min. The sheared sample is subjected to high-pressure puffing (1 time at low pressure of 400 atmospheres, once at medium pressure of 950 atmospheres, and once at high pressure of 1700 atmospheres); 0.3% of dry weight. The pH was adjusted to 5.2 with sodium acetate buffer solution, the treatment time was 24 hours, and the temperature was 42 degrees. The processed sample was centrifuged at 8000r / min for 15min, and the supernatant was taken; an equal volume of ethyl acetate was added to ...
Embodiment 2
[0032] The brewer's grain bran collected from the brewery was dried in a drying oven at 70°C; the dried bran was crushed to a particle size of about 0.3mm with a grinder; 2g of the sieved bran was weighed and sterilized The pot is sterilized for 30 minutes to inactivate its endogenous enzyme activity. Add 40 mL of 0.1N sodium hydroxide solution and soak at 60°C for 12 hours; adjust the pH of the soaked bran to 7.0 with acetic acid, and cut it for 30 minutes with a high-speed shear at 18,000 r / min. The sheared samples were subjected to high-pressure puffing (once at 500 atmospheres, twice at high pressures of 1700 atmospheres); xylanase was added to the treated samples, and the amount of enzyme added was 0.3% of the dry weight of bran. The pH is adjusted to 5.2 with sodium acetate buffer solution, the treatment time is 48 hours, and the temperature is 50 degrees. The treated sample was centrifuged at 4000r / min for 15min, and the supernatant was taken; an equal volume of ethyl ...
Embodiment 3
[0034] The brewer's grain bran collected from the brewery was dried in a drying oven at 70°C; the dried bran was crushed to a particle size of about 0.3mm with a grinder; 2g of the sieved bran was weighed and used for sterilization The pot is sterilized for 30 minutes to inactivate its endogenous enzyme activity. Add 20mL of 0.1N sodium hydroxide and 30ml of distilled water, and soak at 70°C for 6 hours; adjust the pH of the soaked bran to 7.0 with acetic acid, and use a high-speed shearing machine at 18000r / min to cut for 30min; The final sample is subjected to high-pressure puffing (2 times at a low pressure of 500 atmospheres, and 2 times at a high pressure of 1700 atmospheres). Glucanase was added to the treated sample in an amount of 1% of the dry weight of the bran; cellulose and hemicellulase were added in an amount of 0.3% of the dry weight of the bran. The pH was adjusted to 5.2 with sodium acetate buffer solution, the treatment time was 48 hours, and the temperature...
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