Chinese herbal medicine extracting solution used for stevia rebaudiana culture medium and preparation method

A technology of Chinese herbal medicine extract and medium, applied in botany equipment and methods, chemicals for biological control, gardening methods, etc., can solve the problem of reducing the quality and efficiency of tissue culture, failure to form a chain of inhibition, and tissue culture failure and other problems, to achieve the effect of improving autoimmune function, ensuring activity, and good extraction effect

Inactive Publication Date: 2017-05-10
MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned measures to prevent microbial contamination in the tissue culture process all have certain effects, but the disadvantage is that each antibacterial link is isolated and cannot form a complete and unified antibacterial chain. If any link in the process is negligent, microorganisms will take advantage of it. , resulting in failure of tissue culture, reducing the quality and efficiency of tissue culture. In addition, through sterilization of the medium, aseptic inoculation, and cultivation in a sterile environment, the purpose of sterilization and antibacterial is achieved through external operations. In these links Once any link is not in place, bacteria and other microorganisms will be infected. Therefore, a measure of continuous antibacterial function is needed to keep the stevia explants in a sterile environment for a long time, and at the same time enhance the internal antibacterial function of stevia explants Ability to resist contamination by foreign microorganisms

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The Chinese herbal medicine contained in the Chinese herbal medicine extract used for the Stevia rebaudiana medium described in this embodiment includes the following components in parts by weight: 15 parts of Jiangxiang, 9 parts of Piperumba, 10 parts of Shanzi mushroom, 7 parts of Haijinsha, 8 parts of ginger, 7 parts of ten major contributions. The Chinese herbal medicine extract is used for the stevia callus culture medium; the callus culture medium is used for stevia tissue culture using stems as explants.

[0021] The preparation method of the feed additive that is used for preventing and treating suckling pig ascariasis described in the present embodiment, described method comprises the steps:

[0022] (1) Soak the Jiangxiang, Piba, Shanzigu, Haijinsha, Bianjiang and Shigonggong in water for 30 minutes, rinse them twice with water, add ethanol to extract for 3 hours, and filter Afterwards, the pre-extraction solution of Jiangxiang, Piba, Shanzigu, Haijinsha, Bia...

Embodiment 2

[0026] The Chinese herbal medicine contained in the Chinese herbal medicine extract used for the Stevia rebaudiana medium described in this embodiment includes the following components in parts by weight: 10 parts of Dalbergia basilica, 14 parts of Piperumba, 6 parts of Arrowhead mushroom, 10 parts of Haijinsha, 4 parts of ginger, 11 parts of ten major contributions. The Chinese herbal medicine extract is used for the stevia protocorm culture medium; the protocorm culture medium is used for stevia tissue culture using leaves as explants.

[0027] The preparation method of the feed additive that is used for preventing and treating suckling pig ascariasis described in the present embodiment, described method comprises the steps:

[0028] (1) Soak the Jiangxiang, Piba, Shanzigu, Haijinsha, Bianjiang and Shigonggong in water for 20 minutes, rinse with water for 3 times, add ethanol to extract for 2 hours, and filter Afterwards, the pre-extraction solution of Jiangxiang, Piba, Sha...

Embodiment 3

[0032] The Chinese herbal medicine contained in the Chinese herbal medicine extract used for the Stevia rebaudiana medium described in this embodiment includes the following components in parts by weight: 14 parts of Jiangxiang, 11 parts of Piperumba, 9 parts of Arrowhead mushroom, 8 parts of Haijinsha, 7 parts of ginger, 8 parts of ten major contributions. The Chinese herbal medicine extract is used for the stevia subculture medium; the subculture medium is used for the stevia tissue culture using stems as explants.

[0033] The preparation method of the feed additive that is used for preventing and treating suckling pig ascariasis described in the present embodiment, described method comprises the steps:

[0034] (1) Soak the Jiangxiang, Piba, Shanzigu, Haijinsha, Bianjiang and Shigonggong in water for 30 minutes, rinse them twice with water, add ethanol to extract for 3 hours, and filter Afterwards, the pre-extraction solution of Jiangxiang, Piba, Shanzigu, Haijinsha, Bian...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of stevia rebaudiana culture mediums and provides a Chinese herbal medicine extracting solution used for a stevia rebaudiana culture medium and a preparation method. The Chinese herbal medicine extracting solution used for the stevia rebaudiana culture medium is prepared from the following Chinese herbal medicine components: lignum dalbergiae odoriferae, piper longum, pseudobulbus cremastrae seu pleiones, spora lygodii, Bianjiang and mahonia fortunei; the Chinese herbal medicine extracting solution is used for any one of a stevia rebaudiana callus culture medium, a protocorm culture medium, a subculture culture medium, a passage culture medium and a rooting culture medium. All the Chinese herbal medicines are prepared through techniques such as ethanol digestion, boiling and concentration. Aiming at disadvantages of a bacterium-inhibiting measure in existing stevia rebaudiana tissue culture, the Chinese herbal medicines are added into a basic culture medium and the culture medium is enabled to be located in an immune environment, so that pollution caused by microorganisms including external bacteria and the like is prevented, and furthermore, the efficient and lasting bacterium inhibition effect is realized; meanwhile, the resistance of stevia rebaudiana explants is improved, the investment of manpower and material resources is reduced and the cost is reduced. The method is simple to operate and has a good extracting effect.

Description

technical field [0001] The invention belongs to the technical field of stevia culture medium, and in particular relates to a Chinese herbal medicine extract used in stevia culture medium and a preparation method. Background technique [0002] In recent years, stevia tissue culture technology has developed rapidly. In the tissue culture process, there are often microbial contamination problems such as bacteria, resulting in poor quality of stevia tissue culture, low efficiency, and serious nutrition. A problem for people. [0003] The microbial contamination during the stevia tissue culture process mainly comes from two aspects: one is the plant material itself carrying bacteria; the other is caused by the operator and the microorganisms in the air; in addition, the pollution may also be caused by the medium carrying bacteria and the cross contamination of operating tools. caused by pollution. Contaminating microorganisms are divided into two categories: bacteria and fungi....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N65/48A01P1/00A01P3/00A01P21/00
CPCA01H4/001A01N65/00A01N65/48A01N2300/00
Inventor 贾德军
Owner MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products