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Arabidopsis thaliana tracheary element in-vitro induction cultivation method

A technology of Arabidopsis thaliana and liquid culture medium, which is applied in the field of in vitro induction and cultivation of Arabidopsis tubular molecules, which can solve the problems of complicated material collection, long culture time, poor cell dispersion, etc., and achieve a large number of materials, easy material collection, and applicable wide range of effects

Active Publication Date: 2017-05-10
QILU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the in vitro induction and culture of Arabidopsis tubular molecules, and most of them have problems such as complicated material collection, relatively long culture time, and poor cell dispersion.

Method used

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  • Arabidopsis thaliana tracheary element in-vitro induction cultivation method
  • Arabidopsis thaliana tracheary element in-vitro induction cultivation method
  • Arabidopsis thaliana tracheary element in-vitro induction cultivation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Induction of Tubular Molecules in Arabidopsis Columbia Wild-type Lines in Vitro

[0039] The specific method is as follows:

[0040] (1) Sterile inoculation: pick mature and plump Arabidopsis thaliana wild-type seeds, sterilize with 2% sodium hypochlorite for 20 minutes, rinse with sterile water 4 times, and sow on MS basic medium.

[0041] (2) Callus preparation: seedling Arabidopsis thaliana seedlings 10 days after germination (such as figure 1 ) the whole plant was chopped and inserted into the prepared callus induction medium, cultured in the dark at 23° C., subcultured once every 14 days, and co-induced and cultured for 28 days to obtain Arabidopsis callus.

[0042] (3) Callus expansion: transfer the induced Arabidopsis callus into the callus proliferation medium prepared above, culture in the dark at 23°C, subculture once every 15 days, and co-culture for 30 days, so that The Arabidopsis callus underwent rapid proliferation, and the results were as fo...

Embodiment 2

[0045] Example 2: Induction of Tubular Molecules in Arabidopsis Transgenic RABG3f-RFP Gene Lines in Vitro

[0046] The specific method is as follows:

[0047] (1) Sterile inoculation: select mature and plump Arabidopsis thaliana transgenic RABG3f-RFP seeds, sterilize with 2% sodium hypochlorite for 20 minutes, rinse with sterile water for 4 times, and sow on MS basic medium.

[0048] (2) Callus preparation: cut up the whole plant of Arabidopsis thaliana seedlings 12 days after germination and insert them into the prepared callus induction medium, culture in the dark at 25°C, subculture once every 15 days, and co-induce After culturing for 30 days, Arabidopsis callus was obtained.

[0049] (3) Callus expansion: transfer the induced Arabidopsis callus into the above-mentioned prepared callus proliferation medium, culture in the dark at 25°C, subculture once every 15 days, and co-culture for 30 days, so that Arabidopsis callus undergoes rapid proliferation.

[0050] (4) Callus...

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Abstract

The invention discloses an arabidopsis thaliana tracheary element in-vitro induction cultivation method. The arabidopsis thaliana tracheary element in-vitro induction cultivation method comprises the following steps of (1) preparing an arabidopsis thaliana callus tissue; (2) performing propagation expanding cultivation on the arabidopsis thaliana callus tissue on a callus tissue proliferation medium; (3) performing suspension cultivation on the arabidopsis thaliana callus tissue subjected to propagation expansion to obtain an arabidopsis thaliana callus tissue suspension cultivation system with high dispersibility; and (4) filtering the arabidopsis thaliana callus tissue suspension cultivation system by a cell strainer, transferring a monocellular cultivation system after filtration to an arabidopsis thaliana tracheary element in-vitro induction liquid culture medium, performing induction cultivation, and inducing the single cell of the arabidopsis thaliana callus tissue to be differentiated and form a tracheary element in vitro. According to the method provided by the invention, the application range of the arabidopsis thaliana materials is wider, materials are simpler and easier to obtain, the cultivation time is shortened, and the dispersibility of the obtained cell is higher.

Description

technical field [0001] The invention relates to a method for inducing and culturing Arabidopsis tubular molecules in vitro, and belongs to the technical field of plant tissue culture. Background technique [0002] The development of biomass energy is an effective way to deal with the energy crisis. The cell wall component is the biomass with the highest yield on the earth. The main components of the cell wall are cellulose, hemicellulose and lignin, among which lignin seriously hinders the effective utilization of cellulose and hemicellulose. Therefore, the research on the mechanism of cell wall formation and Composition modification is conducive to the efficient utilization of biomass energy. [0003] Tubular molecules are the main functional structures of plant xylem tissue—including tracheids and vessels, which are responsible for the long-distance transport of water absorbed by the root to the shoot. The striated thickening of the side wall of the tubular molecule is t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 蒋苏李师鹏曹子谊赵淑举刘振东白如水
Owner QILU NORMAL UNIV
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