A method for rapid breeding of Azalea spp. by regenerating adventitious buds from leaves of tissue cultured seedlings

Abstract

The invention belongs to the technical field of abricultural living things and relates to a method for quickly breeding rhododendron molle by an approach of regenerating adventitious buds by utilizing tissue culture seedling leaves. The apical third to fifth leaves of the sterile tissue culture seedlings of the rhododendron molle serve as explants, the leaves vertical to the main vein are cut partially and petiole and leaf tip are cut off, the leaves are inoculated on a WPM culture medium of additional hormones TDZ of 1.0 mg / L and IAA of 1.0 mg / L, illumination culture is conducted after dark culture is conducted for 10 to 15 days, and the induction rate of the adventitious buds of the leaves reaches above 85 percent and the average induction number of the adventitious buds of is 7.2 pieces per leaf when the illumination culture is conducted for 35 days. The adventitious buds grow on the WPM culture medium added with ZT of 1.0 mg / L and IBA of 0.1 to 0.5 mg / L to form seedlings, the seedlings develop on the 1 / 2WPM culture medium added with IBA of 10 to 12.5 mg / L and NAA of 0.1 mg / L to form integrated small plants with roots, stems and leaves, and the surviving plant rate reaches above 95 percent. The method is suitable for rhododendron molle test-tube plantlet industrialized production and establishment of an agrobacterium mediated genetic transformation system.

Description

technical field [0001] The invention relates to a method for rapid breeding of azaleas by using the method of regeneration of adventitious buds from leaves of tissue cultured seedlings, and belongs to the field of agricultural biotechnology. Background technique [0002] Blume (Rhododendron molle (Blume) G.Don), also known as blume, yellow rhododendron, and yellow azalea, belongs to the deciduous shrub of Rhododendron molle (Blume) subgenus, Rhododendron molle (Blume) in the family Rhododendron. The only native species. Azalea is an excellent landscaping tree species to be developed. It can be introduced and domesticated to become a horticultural ornamental plant cultivated in the open field. At the same time, it is also a rare and endangered medicinal plant. The organs of the whole plant contain a variety of active ingredients. The flowers, roots, and fruits are all available for medicinal purposes. It has the functions of dispelling dampness, removing wind, and relieving ...

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Problems solved by technology

The domestic research on tissue culture and rapid propagation of Deer deer is to obtain sterile tissue culture seedlings by cutting the branches of Deer deer, and further establish the establishment of Deer deer by cutting the stem nodes of the tissue culture seedlings, inducing and cultivating clusters of axillary buds on the tender stem segments. In vitro culture and plant regeneration system of deer, the proliferation rate of bud clusters is more than 4-5 times (Gu Honghui, Yuan Qunying, Zhu Chunyan, Zhu Danhua. Tissue culture and rapid propagation of deer[J]. Plant Physiology Communications, 2006, 42(4):683; Gu Dizhou, Lu Shuang, Ba Chunying, Li Yuanyuan. In Vitro Culture and Plantlet Regeneration of Tender Stems of A. chinensis[J]. Journal of Shandong Agricultural University (Natural Science Edition), 2010,43(4 ):574-578); The rapid propagation system established by inducing and cultivating the axillary buds of the tender stem section can not be used as the genetic transformation system mediated by Agrobacterium to carry out genetic improvement of Oats.

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