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Paeonia rockii primary culture callus induction method

A callus induction and peony technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of easy browning, low callus induction rate, etc., to reduce the browning rate and improve the induction rate. rate, the effect of promoting the establishment

Inactive Publication Date: 2017-05-10
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, the callus induction rate is low due to the fact that the callus is easily browned when the callus is induced by the scale bud.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Selection of explants: choose "purple spot" oil and use peony scale buds as explants, soak them in washing powder solution for 2 hours, then gently scrub the outer surface of the scale buds with a soft brush, and then rinse them under running water for 24 hours. Then wrap it with gauze and put it in the refrigerator (0-4°C) to pre-cool for 24 hours.

[0019] 2. Disinfection and inoculation of explants: Transfer the explants to the aseptic operating table, peel off the scales of the buds, first disinfect with 75% alcohol for 20 seconds, then disinfect with 0.1% mercury liter for 10 minutes, and then use sterile After washing with bacteria water for 5 times, they were inoculated on the callus induction medium, and one stem segment was connected to each glass bottle. The composition of the callus induction medium is MS medium, 6-BA1mg / L, NAA0.3mg / L, silver nitrate 4mg / L, 7g / L agar, 30g / L sucrose; the pH of the medium is adjusted to 5.8.

[0020] 3. Callus induction cul...

Embodiment 2

[0023] 1. Selection of explants: choose "purple spot" oil and use peony scale buds as explants, soak them in washing powder solution for 2 hours, then gently scrub the outer surface of the scale buds with a soft brush, and then rinse them under running water for 24 hours. Then wrap it with gauze and put it in the refrigerator (0-4 ℃) to pre-cool for 48 hours.

[0024] 2. Disinfection and inoculation of explants: Transfer the explants to the aseptic operating table, peel off the scales of the buds, first disinfect with 75% alcohol for 30 seconds, then disinfect with 0.1% mercury liter for 15 minutes, and then use sterile After washing with bacteria water for 5 times, they were inoculated on the callus induction medium, and one stem segment was connected to each glass bottle. The composition of the callus induction medium is MS medium, 6-BA2mg / L, NAA0.3mg / L, silver nitrate 5mg / L, 7g / L agar, 30g / L sucrose; the pH of the medium is adjusted to 5.8

[0025] 3. Callus induction cult...

Embodiment 3

[0028] 1. Selection of explants: choose "purple spot" oil and use peony scale buds as explants, soak them in washing powder solution for 2 hours, then gently scrub the outer surface of the scale buds with a soft brush, and then rinse them under running water for 24 hours. Then wrap it with gauze and put it in the refrigerator (0-4 ℃) to pre-cool for 36 hours.

[0029] 2. Disinfection and inoculation of explants: Transfer the explants to the aseptic operating table, peel off the scales of the buds, first disinfect with 75% alcohol for 25 seconds, then disinfect with 0.1% mercury liter for 12 minutes, and then use sterile After washing with bacteria water for 5 times, they were inoculated on the callus induction medium, and one stem segment was connected to each glass bottle. The composition of the callus induction medium is MS medium, 6-BA1.5mg / L, NAA0.4mg / L, silver nitrate 4.5mg / L, 7g / L agar, 30g / L sucrose; the pH of the medium is adjusted to 5.8

[0030] 3. Callus induction ...

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PUM

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Abstract

The invention discloses a paeonia rockii primary culture callus induction method. The paeonia rockii primary culture callus induction method comprises the following steps: (1) explant selection: selecting bulbel of paeonia rockii as an explant, washing, and pre-cooling for 24 to 48 hours; (2) sterilizing and inoculating: stripping bud scales off from the bulbel obtained in step (1) under an aseptic condition, sterilizing, and then inoculating the bulbel to a callus induction culture medium; (3) callus induction culture: putting the inoculated explant in a culture room for culturing, and culturing for 7 to 10 days by using red light. Compared with the prior art, the paeonia rockii primary culture callus induction method disclosed by the invention has the advantages that an explant pre-cooling method is adopted, specific culture medium components are combined, a red-light culture method is finally adopted, the browning rate of the paeonia rockii can be effectively reduced to be 0, no browning phenomenon happens, the callus induction rate is increased to be 100 percent, and cluster buds which are large in quantity and good in quality can be finally obtained, so that the requirements of multiplication can be met, and the establishment of a rapid propagation system is promoted.

Description

technical field [0001] The invention relates to a method for inducing callus of primary cultured peony purpura, belonging to the technical field of peony propagation. Background technique [0002] 'Purple spot' peony is a cultivar commonly used in the production of wild Yangshan peony. 'Purple spot' peony not only has good ornamental and medicinal effects, but also has high oil value. In recent years, relevant investigations have found that 'Purple Spot' peony can also be developed and utilized as woody grain and oil plant resources. The general oil yield of peony for oil is 144 catties / mu, which is much higher than that of sesame and camellia oleifera. 'Purple spot' peony seed oil is rich in unsaturated fatty acids, linolenic acid, linoleic acid and oleic acid, among which the content of α-linolenic acid is as high as 42.34% to 49%. α-linolenic acid is an important component of human brain cells and tissue cells. It is an indispensable polyunsaturated fatty acid that cann...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王姗鲍荣静鲍华鹏颜志明雷武生贾思振王全智
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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