Preparation for protecting activity of superoxide dismutase (SOD) and preparation method of preparation

Abstract

The invention relates to a preparation for protecting activity of superoxide dismutase (SOD) and a preparation method of the preparation. The preparation for protecting the activity of the SOD contains the following raw materials: SOD, sodium hyaluronate, carbomer, glycerol, triethanolamine, imidazolidinyl urea, trehalose and deionized water. The preparation method comprises the steps: soaking the carbomer with water, sequentially adding trehalose and glycerol into the soak solution after soaking is completed, carrying out uniform stirring, and then, carrying out sterilization, so as to obtain a base solution; adding sodium hyaluronate into the SOD, carrying out uniform stirring, and carrying out filtration and sterilization, so as to obtain an SOD sodium hyaluronate solution for later use; sequentially adding the SOD sodium hyaluronate solution, triethanolamine and imidazolidinyl urea into the base solution under sterile conditions, then, adding the deionized water into the solution for adjusting, carrying out uniform mixing and stirring, carrying out sealing, and carrying out emulsification, thereby obtaining the preparation for protecting the activity of the SOD. The preparation and the preparation method thereof have the beneficial effects that all the raw materials of the preparation provided by the invention are combined together, so that the activity of the SOD can be effectively protected; the preparation cost is relatively low.

Description

technical field [0001] The invention relates to a biological product, in particular to a preparation for protecting the activity of superoxide dismutase and a preparation method thereof. Background technique [0002] Superoxide dismutase alias liver protein, referred to as: SOD. SOD is an active substance derived from living organisms, which can eliminate harmful substances (superoxide anion free radicals) produced by organisms during metabolism. The most basic feature of SOD is that it has biological activity, and it exists widely in most aerobic organisms. Its main function is to remove excessive superoxidative substances generated in the body, and it has an important function of antioxidant enzymes. It is undisputed that among all the enzymes discovered so far, it is the only biologically active enzyme that can disproportionate superoxide anion free radicals. It is known as the nemesis of free radicals and the "protector" of human health. [0003] In recent years, with ...

AI Technical Summary

Problems solved by technology

However, the existing freeze-drying technology is too complicated and the equipment is expensive, which increases the cost of protecting SOD activity; and about 10% of the activity of SOD is lost during the freeze-drying process, and the additives added during freeze-drying limit the application of SOD. If a large amount of mannitol is added during freeze-drying, it will limit the application of SOD in cosmetics, and the activity of freeze-dried SOD will be weakened.