A method for efficiently enriching phenolic acids in traditional Chinese medicine by nanofiltration

A technology of nanofiltration and phenolic acid, which is applied in the field of medicine, can solve the problems of membrane pore clogging, low separation efficiency, shortening the service life of nanofiltration membranes, etc., and achieve the effects of increasing service life, reducing adsorption effect and improving membrane fouling

Active Publication Date: 2019-03-19
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

Its separation principles include the Daonan effect, pore model, dissolution-diffusion separation model, etc. When enriching and concentrating phenolic acids in traditional Chinese medicine, most of the phenolic acids can be intercepted and enriched through the pore size sieved pore model, while for The smaller the molecular weight cut-off effect is poor, as found in the enrichment of p-coumaric acid during the experiment, the cut-off rate of the nanofiltration membrane with a molecular weight cut-off of 150Da is lower than 50%. Moreover, macromolecular components are likely to cause membrane pore blockage, which affects the separation efficiency and shortens the service life of the nanofiltration membrane.
[0004] The method currently adopted is to adjust the dissociation ratio of the phenolic acid components, enhance the Donnan effect between the components and the nanofiltration membrane, and improve the component rejection rate. After testing, it is found that when the p-coumaric acid is dissociated by 90%, the molecular weight cut-off The cut-off rate of 150Da nanofiltration membrane is 72.3%, and the loss is obvious
Therefore, although nanofiltration separation has many advantages, due to the limitation of the molecular structure and molecular weight of the solute and the molecular weight cut-off of the nanofiltration membrane, there is still loss of small molecule traditional Chinese medicine phenolic acids during nanofiltration enrichment.
[0005] In the conventional understanding of the separation of traditional Chinese medicine, the addition of ethanol after the acidic component is salted will produce an alcohol precipitation effect, resulting in the loss of components

Method used

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  • A method for efficiently enriching phenolic acids in traditional Chinese medicine by nanofiltration
  • A method for efficiently enriching phenolic acids in traditional Chinese medicine by nanofiltration
  • A method for efficiently enriching phenolic acids in traditional Chinese medicine by nanofiltration

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Embodiment 1

[0018] Example 1: Nanofiltration enrichment of p-coumaric acid in reed rhizome

[0019] The pKa value that adopts potentiometric method to detect p-coumarin acid (concentration reference p-coumaric acid concentration in reed rhizome extract) is 5.41, and the solution pH value range when it dissociates 50%~90% is 5.41~6.63; Adopt 10 % sodium hydroxide aqueous solution regulates the pH value of the reed rhizome extract to 5.41~6.63; Separation pressure (0.5, 1.0, 1.5Mpa) was used as the investigation index, and polyamide composite nanofiltration membrane was used to separate coumaric acid and total phenolic acid by nanofiltration, and the response value of the cut-off rate was selected to screen the nanofiltration separation parameters. In the nanofiltration membrane, under the conditions of pH 6.52 and operating pressure of 1.12Mpa, the rejection rates of p-coumaric acid and total phenolic acids were 72.3% and 85.6% respectively, and the loss of p-coumaric acid was obvious; und...

Embodiment 2

[0023] Example 2 Nanofiltration Enrichment of Phenolic Acids in Salvia Miltiorrhiza Extract

[0024] Adopt spectrophotometry to detect that the pKa value to rosmarinic acid (concentration reference salvia miltiorrhiza extract) is 4.01, and the solution pH value range when it dissociates 50%~90% is 4.01~4.96; Adopt 10% Sodium hydroxide aqueous solution adjusts the pH value of the reed rhizome extract to 4.01-4.96; adopts the corresponding surface analysis method to separate the The pressure (0.5, 1.0, 1.5Mpa) was used as the investigation index, and the sulfonated polyethersulfone nanofiltration membrane was used to separate rosmarinic acid and total phenolic acid by nanofiltration, and the response value of the cut-off rate was selected to screen the parameters of the nanofiltration separation. In the 450Da nanofiltration membrane, the pH value is 4.77, and the operating pressure is 1.08Mpa, the retention rates of rosmarinic acid and total phenolic acid are 82.2% and 86.1% res...

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Abstract

The invention provides a method for high-efficiency enrichment of phenolic acid components in a traditional Chinese medicine through nanofiltration. According to the invention, the method is provided on the basis of the fact that the state of phenolic acid in a traditional Chinese medicine existing in a solution is related to nanofiltration interception behaviors; the pKa of to-be-enriched phenolic acid components is detected, acid-base adjustment is carried out so as to allow the degree of dissociation to reach 50 to 90%, and ethanol is further added; so the Donnan effect between dissociated phenolic acid and a nanofiltration membrane is improved on the premise of no alcohol precipitation effect, and membrane flux is increased and separating efficiency is improved while the molecular cut off of nanofiltration is increased. Thus, the method improves the enrichment efficiency of phenolic acid in the traditional Chinese medicine, overcomes the problem of poor enrichment effect of micromolecular phenolic acid separated through nanofiltration, mitigates adsorption effect between a solute and the nanofiltration membrane in a low-concentration ethanol solution, enhances membrane contamination and prolongs the service life of the nanofiltration membrane.

Description

technical field [0001] The invention relates to a method for enriching phenolic acid components of traditional Chinese medicine, in particular to a method for enriching phenolic acid components of traditional Chinese medicine by using nanofiltration technology based on the interaction law of physical and chemical parameters of traditional Chinese medicine components and nanofiltration membranes, and belongs to the field of medicine. Background technique [0002] Phenolic acids in traditional Chinese medicine contain phenolic hydroxyl groups, double bonds, etc. in their structures, which are thermally unstable. They have been transformed and decomposed during the process of component separation and purification, which affects product quality and causes waste of resources. Such as chlorogenic acid in honeysuckle, salvianolic acid components in salvia miltiorrhiza, etc., all have the above-mentioned problems in the process of processing. [0003] The molecular weight cut-off of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/47C07C59/52C07C67/56C07C69/732A61K36/899A61K36/537
CPCA61K36/537A61K36/899C07C51/47C07C67/56C07C59/52C07C69/732
Inventor 李存玉彭国平李红阳
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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