CdSe/ZnS quantum dot nano-cluster based electrochemiluminescence biological sensor, as well as manufacturing method and application thereof

A technology of luminescent biology and quantum dots, applied in chemiluminescence/bioluminescence, chemical instruments and methods, nano optics, etc., to achieve the effects of improving sensitivity, rapid analysis and detection, and excellent accuracy

Inactive Publication Date: 2017-05-10
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the combination of hybridization chain reaction amplif

Method used

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  • CdSe/ZnS quantum dot nano-cluster based electrochemiluminescence biological sensor, as well as manufacturing method and application thereof
  • CdSe/ZnS quantum dot nano-cluster based electrochemiluminescence biological sensor, as well as manufacturing method and application thereof
  • CdSe/ZnS quantum dot nano-cluster based electrochemiluminescence biological sensor, as well as manufacturing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Preparation of Electrochemiluminescent Biosensor and Its Detection of Cancer Cells

[0039] First, the magnetic gold electrodes were sequentially used α-Al of 1.0 μm, 0.3 μm and 0.05 μm 2 o 3 After polishing with polishing powder, ultrasonically wash with double distilled water and let it dry naturally, drop 8 μL of magnetic nano gold rod solution onto the surface of the electrode and let it dry naturally. Then 10 μL of aptamer DNA (C1) was dropped onto the surface of the electrode and incubated for 10 h.

[0040] Wash the electrode with the above-mentioned aptamer and immerse it into the nano-gold-DNA priming strand (solution a), incubate with shaking at 37°C for 2h, and then immerse the electrode into the CdSe / ZnS quantum dot-hair chain after cleaning. Clamp the DNA signal probe (solution b) and incubate with shaking at 37° C. for 2 hours to make a biosensor for detecting ECL of cells.

[0041] Incubate the ECL sensor with 100 μL of PBS solution containi...

Embodiment 2

[0042] Example 2. Preparation of Electrochemiluminescent Biosensor and Its Detection of Cancer Cells

[0043] "Drop 10 μL of aptamer DNA (C1) onto the surface of the electrode and incubate for 10 h" to "drop 10 μL of aptamer DNA (C1) on the surface of the electrode and incubate for 12 h". Other preparation conditions were the same as in Example 1, and a biosensor similar in appearance and properties to Example 1 was obtained. The results of cancer cell detection are the same as in Example 1.

Embodiment 3

[0044] Example 3. Preparation of Electrochemiluminescent Biosensor and Its Detection of Cancer Cells

[0045] "Incubate the ECL sensors with 100 μL of PBS solutions containing different concentrations of cells at 37°C for 60 minutes with shaking" was changed to "Incubate the ECL sensors with 100 μL of PBS solutions with different concentrations of cells at 37°C for 50 minutes with shaking". Other preparation conditions were the same as in Example 1, and a biosensor similar in appearance and properties to Example 1 was obtained. The results of cancer cell detection are the same as in Example 1.

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Abstract

The invention discloses a CdSe/ZnS quantum dot nano-cluster based electrochemiluminescence (ECL) biological sensor, as well as a manufacturing method and application thereof to cancer cell detection. According to the technical scheme of the invention, the ECL biological sensor is manufactured by the steps of modifying a magnetic nano-gold rod on the surface of a gold electrode, connecting a cell aptamer, then assembling a nano-gold-DNA initiating chain and finally fixing a CdSe/ZnS quantum dot nano-cluster ECL signal probe. The sensor provided by the invention is washed and then subjected to oscillation warm bath with 100 uL of PBS solution containing cancer cells with different concentration for 45 to 60 minutes, an ECL test is conducted, and the luminescent intensity and the concentration of to-be-tested samples (the cancer cells) form a linear relationship. An nano-gold amplification effect and a hybrid chain type reaction amplification technology are combined, a large amount of CdSe/ZnS quantum dots are assembled on a nano-line, the ECL signal is greatly amplified, the cancer cells are subjected to high-sensitivity and high-selectivity detection, and the method has huge application potential in early clinical detection of the cancer cells.

Description

[0001] Technical field: [0002] The invention relates to a new method for developing a novel electrochemiluminescent biosensor based on CdSe / ZnS quantum dot nano-clusters, and an analysis method for detecting cancer cells by using the electrochemiluminescent biosensor. [0003] Background technique: [0004] Electrochemiluminescence (ECL) has many excellent performance characteristics such as simple operation, fast speed, high sensitivity and good controllability. As a new type of analysis and detection device, ECL biosensor is gradually being used in various biological analysis and detection. [Piper, D.J.E.; Barbante, G.J.; Brack, N.; Pigram, P.J.; Hogan, C.F. Langmuir 2011, 27, 474–480.]. Recently, ECL biosensors and bioanalysis technologies using quantum dots as luminescent materials have been widely used in the field of analytical science due to their remarkable ECL luminescent properties, good biocompatibility, and their own nano-amplification effect. [Lei, J.; Ju, H. Tr...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/38C09K11/88B82Y20/00B82Y30/00B82Y40/00
Inventor 接贵芬路正坤
Owner QINGDAO UNIV OF SCI & TECH
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