Method for submicron-scale in-situ characterization of soil microaggregate

Abstract

The invention discloses a method for submicron-scale in-situ characterization of soil microaggregate and belongs to the field of soil fertility improvement and soil organic matter accumulation. The method includes: extracting to obtain soil microaggregate, freezing, embedding, slicing, and then putting on a silicon nitride thin film window; respectively using a scanning electron microscope, synchronous radiation infrared microimaging technology and nano secondary ion probe technology to sequentially analyze a same area-of-interest selected by soil microaggregate slices so as to respectively acquire information of in-situ inorganic-organic functional group composition, element binding site and spatial distribution, wherein an important basis is provided for studying sequestration of soil organic carbon and organic matter improving mechanism. According to the method, the silicon nitride window is creatively used as a soil microaggregate slice carrier for the first time, observing of a same area by applying three characterization methods at the same time is realized, and the problems of huge damage, high complexity, low synchronism, low visuality and unmatched in-situ dimension that conventional soil microaggregate studying methods have are improved.

Description

technical field [0001] The invention is a method for characterizing soil micro-aggregates in situ at a submicron scale, and belongs to the fields of soil fertility improvement and soil organic matter accumulation. Background technique [0002] Soil microaggregates are the structural units smaller than 0.25mm formed by coagulation, adhesion, and cementation of the original mechanical components in the soil. They are the most active parts of the organic and inorganic components of the soil, and are also important for various physical and biochemical reactions in the soil. Carrier, so it is an important indicator to study the improvement of soil fertility and the accumulation of soil organic matter. Due to the small size of soil micro-aggregates and the adhesion of inorganic and organic elements, traditional chemical extraction, mass spectrometry and other methods will inevitably cause disturbance and damage to the composition and structure of soil micro-aggregates, and there i...

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Problems solved by technology

Due to the small size of soil micro-aggregates and the adhesion of inorganic and organic elements, traditional chemical extraction, mass spectrometry and other methods will inevitably cause disturbance and damage to the composition and structure of soil micro-aggregates, and there is a lack of in-situ simultaneous visualization of soil micro-aggregates. ability to aggregate inorganic organic elements

With the development and application of synchrotron radiation infrared spectroscopy and secondary nano-ion probes, traditional low-radiation glass slides cannot observe the same area by two methods at the same time. Simultaneously studying important information such as inorganic and organic functional groups, element binding sites, and spatial distribution of soil microaggregates has become a key technology that needs to be solved urgently in this field.

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