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Efficient exercising technique for Bletilla striata tissue culture seedlings

A technology for tissue culture and hardening of seedlings, applied in cultivation, culture medium, planting substrate, etc., can solve the problems of long domestication time and hardening time, and achieve the effects of fast growth, high survival rate, and large pseudobulbs

Active Publication Date: 2017-05-24
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The seedling hardening technology for white and tissue cultured seedlings has been announced in the above-mentioned documents, which has improved the survival rate of hardened seedlings, but there is a problem that the domestication time is longer when hardening seedlings, and the hardening time is too long. The seedling hardening time is 3 days. month to 5 months

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  • Efficient exercising technique for Bletilla striata tissue culture seedlings
  • Efficient exercising technique for Bletilla striata tissue culture seedlings
  • Efficient exercising technique for Bletilla striata tissue culture seedlings

Examples

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Embodiment 1

[0033] A kind of high-efficiency seedling hardening technology of white and tissue cultured seedlings, its specific steps are:

[0034] (1) Seedling hardening in the sun shed of bottle seedlings: After being transferred to the rooted and strong seedlings for about 1 month, the tissue culture seedlings of Baiji tissue culture seedlings have initially formed pseudobulbs and root systems, and the tissue culture seedlings with ≥ 2 leaves can be used in the sun shed To harden the seedlings, move the tissue cultured seedlings together with the container to the greenhouse or greenhouse (cover with a layer of sunshade net, the light transmittance is 50% to 70%), and control the temperature at 10°C to 30°C to make the tissue cultured seedlings adapt to the environment. Natural light and temperature difference between day and night. After sealing the seedlings for 20 days, when the pseudobulbs of the tissue culture seedlings are ≥ 3 mm, the number of leaves is ≥ 2 pieces, and the plant ...

Embodiment 2

[0042] A kind of high-efficiency seedling hardening technology of white and tissue cultured seedlings, its specific steps are:

[0043] (1) Seedling hardening in the sun shed: Transfer to the medium for rooting and strong seedlings and cultivate for 1 month. The pseudobulb and root system have initially formed, and the tissue culture seedlings with ≥ 2 leaves are moved to the greenhouse or greenhouse together with the container ( Cover with a layer of sunshade net, the light transmittance is 50% to 70%), and the temperature is controlled at 10°C to 30°C, so that the tissue culture seedlings can adapt to the natural light and the temperature difference between day and night. After sealing the seedlings for 30 days, when the pseudobulbs of the tissue culture seedlings are ≥ 3 mm, the number of leaves is ≥ 2, and the plant height is ≥ 6 cm, the robust tissue culture seedlings can be transplanted.

[0044] (2) the preparation of transplanting seedbed and matrix of white and tissue...

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Abstract

The invention discloses an efficient exercising technique for Bletilla striata tissue culture seedlings and belongs to the technical field of planting of traditional Chinese medical materials; the technique comprises the main steps of (1) exercising bottle seedlings in a sun shed; (2) preparing an exercising bed and matrix; (3) transplanting tissue culture seedlings; (4) managing after transplanting. Compared with other exercising and transplanting techniques for Bletilla striata tissue culture seedlings, the efficient exercising technique enables tissue culture seedlings to adapt to natural lighting and day-night temperature differences, and also significantly promotes the expansion of Bletilla striata pseudobulbs; the transplanting matrix is made by spreading bark fragments of different sizes layer by layer, a planting method wherein 2-3 plants are planted in a group is used so that survival rate of the Bletilla striata tissue culture seedlings transplanted is effectively increased; post-transplanting seedling exercising takes a short time, up to 2 months and longer; the pseudobulbs grow fast, and within 2 months of transplanting, the pseudobulbs increase from 5.66 mm to 10.50 mm in average diameter and grow to 4.84 mm averagely; the average survival rate of the tissue culture seedlings is up to 95% and above, field survival rate is 97% and above, and production efficiency and seedling quality are increased effectively at the premise of ensuring high survival rate.

Description

technical field [0001] The invention belongs to the technical field of planting Chinese medicinal materials, and in particular relates to a high-efficiency seedling hardening technology for tissue-cultured seedlings of bletilla chinensis. Background technique [0002] Baiji (Bletilla striata (Thunb.) Reichb.f.) is a perennial herb belonging to Orchidaceae Baiji, also known as Baiji, Lianjicao, Baigi, etc. It is mainly produced in Guizhou, Sichuan, Yunnan, Hunan, Hubei, Anhui Wait for the province. Dried tubers are used as medicine, which is a traditional Chinese medicine commonly used in my country. It has the effects of stopping bleeding and astringent, clearing heat and dampness, reducing swelling and promoting muscle growth. Indications for pulmonary tuberculosis hemoptysis, bronchiectasis hemoptysis, gastric ulcer hematemesis, hematuria, hematochezia embolism; external use for traumatic hemorrhage, burns, chapped hands and feet embolism. In addition, the polysaccharide...

Claims

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Application Information

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IPC IPC(8): A01G31/00
CPCA01G24/00A01G24/23A01G31/00
Inventor 张智慧刘大会朱新焰季鹏章王家金王丽马聪吉
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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