Method for achieving simultaneous detection of chemical structure and biological activity of functional component of food
A technology of biological activity and chemical structure, applied in the preparation of test samples, analytical materials, measuring devices, etc., can solve the problems of inability to elucidate the biological activity of complex biologically active functional components, and achieve the effect of a simple and efficient detection method
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[0044] Preferably, the preparation method of silver dendritic nanoparticles comprises: putting the zinc sheet into AgNO with a concentration of 100-200mmol / L 3 In the solution, the reaction time is 20-60s.
[0045] In a specific embodiment of the present disclosure, the active ingredient may be nobiletin, 5-desmethylnobiletin, 4'-desmethylnobiletin, 5,4'-didemethylnobiletin polymethoxyflavone compounds such as tangeretin, tangeretin or 5-desmethyltangeretin, polymethoxyflavone glycosides, phenols and terpenes, etc.; specifically, the polymethoxyflavone glycosides can be is hesperidin, the phenols may be synephrine, and the terpenes are β-carotene.
[0046] A second aspect of the present disclosure provides a method for detecting active ingredients in food, comprising the following steps:
[0047] S1: Using the method described in the first aspect of the present disclosure to obtain the characteristic barcode B of the active ingredient in the sample to be tested;
[0048] S2...
Embodiment 1
[0053] This example is the simultaneous detection of the chemical structure and antioxidant activity of 6 polymethoxylated flavones
[0054] (1) Preparation of 6 kinds of polymethoxylated flavone standard solution
[0055] 6 polymethoxylated flavones (chemical structures such as figure 1 shown) are nobiletin, 5-desmethylnobiletin, 4'-desmethylnobiletin, 5,4'-didemethylnobiletin, tangeretin and 5-desmethylnobietin The 6 compounds including corticosteroids are all directly extracted and isolated from citrus peel. These components can be pre-dissolved with a small amount of chromatographic grade dichloromethane, and then diluted with chromatographic grade methanol to a concentration of 100ppm for later use.
[0056] (2) SERS characterization of functional components of polymethoxylated flavones
[0057] Firstly, all the glassware were ultrasonically cleaned and soaked in ultrapure water, and dried in an oven for later use. Accurately weigh 3.39gAgNO 3 Dissolve the crystals i...
Embodiment 2
[0065] Chemical structure of functional ingredients of polymethoxyflavone glycosides, phenols and terpenes (chemical structures such as figure 1 shown) and simultaneous detection of biological activity.
[0066] (1) Preparation of standard solution of functional ingredients of polymethoxyflavone glycosides, phenols and terpenes
[0067] Hesperidin is a flavonoid substance with glycosides, its solubility is similar to that of polymethoxylated flavones, it is pre-dissolved in chromatographic dichloromethane, and then diluted to 100ppm with chromatographic methanol for later use. The phenolic substance synephrine is a water-soluble citrus functional ingredient, which is prepared as a 100ppm sample solution to be tested by using ultrapure water and heating and stirring. β-carotene is an isoprene food functional ingredient, which is mainly soluble in organic solvents such as chloroform. In this experiment, dichloromethane was used to pre-dissolve and then diluted with n-hexane to ...
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