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A method and device for evaluating carbon dioxide tolerance of microalgae

A carbon dioxide and tolerance technology, applied in the biological field, can solve the problems of large and unsuitable algae strain screening, large quantities, etc.

Active Publication Date: 2019-04-19
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, more evaluation processes are to cultivate microalgae in triangular flasks or columnar photobioreactors, and compare light absorption values ​​to evaluate the growth performance of microalgae (C12N1 / 12 (2006.01) I, C12Q1 / 04 (2006.01) I), and the larger volume of the triangular flask and columnar photobioreactor is not suitable for the screening of large batches of algae strains, and the sampling work needs to drop a lot of manpower and material resources for batches of algae strains

Method used

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  • A method and device for evaluating carbon dioxide tolerance of microalgae
  • A method and device for evaluating carbon dioxide tolerance of microalgae
  • A method and device for evaluating carbon dioxide tolerance of microalgae

Examples

Experimental program
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Effect test

Embodiment 1

[0038] A method for evaluating the tolerance of algae strains to high concentrations of carbon dioxide, in terms of CO 2 The concentration is 10% (the ratio of carbon dioxide volume to gas volume) as an example, but it does not constitute a limitation to the present invention. This method can be applied to a carbon dioxide concentration between 1-20%. The steps are:

[0039] 1. Culture medium preparation:

[0040] The expansion medium used in the experiment is a conventional medium, such as the medium for Chlorella, Scenedesmus, etc. is BG-11, while the medium for the carbon dioxide tolerance experiment based on the orifice plate is a carbon-free medium, that is, BG-11 does not add Na 2 CO 3 . In this implementation case, Chlorella is taken as an example, and the components of the expansion medium are as follows:

[0041] Table 1 BG-11 medium components for seed expansion

[0042]

[0043] *The formula is shown in Table 2.

[0044] Table 2 Nutrient composition and conc...

Embodiment 2

[0064] according to figure 2 , Figure 3A , Figure 3B , Figure 3C , Figure 3D It can be seen that a device for evaluating the carbon dioxide tolerance of microalgae consists of an LED lamp tube 1, an air outlet 2, an air inlet 3, a light-transmitting culture cylinder 4, a light-transmitting hole plate 5, a moisturizing wave sponge 6, and a culture Cylinder upper opening 7, light-transmitting glass cover 8 are formed. It is characterized in that: the light-transmitting culture cylinder 4 is connected with the light-transmitting glass cover 8 and the LED lamp tube 1 respectively, the left and right sides of the light-transmitting culture cylinder 4 are respectively provided with an air inlet 3 and an air outlet 2, and the light-transmitting culture cylinder 4 The interior is connected with the moisturizing wave sponge 6, and the moisturizing wave sponge 6 is connected with the light-transmitting hole plate 5. The light-transmitting glass cover 8 is light-transmitting bel...

Embodiment 3

[0066] according to figure 1It can be seen that a small-scale photobioreactor device applied to the rapid expansion of microalgae consists of a filter 9, a ventilation duct 10 (4-8), a six-way gas splitter 11, and a perforated rubber plug 12 (4 -8), glass tubes 13 (4-8), light-transmitting glass tubes 14 (4-8), and six-hole shelves 15. It is characterized in that: the light-transmitting glass tube 14 is respectively connected with the perforated rubber plug 12 and the six-hole shelf 15, the perforated rubber plug 12 is connected with the glass tube 13, the glass tube 13 is connected with the ventilation duct 10, and the six-way gas distribution device 11 The upper end and the lower end are respectively connected with the ventilation conduit 10, and the ventilation conduit 10 at the upper end is connected with the filter 9, and also includes a 100ml transparent glass tube 14, and also includes a perforated rubber stopper 12 arranged at the mouth of the transparent glass tube 14...

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Abstract

The invention discloses a method and device applied to assessment of carbon dioxide tolerance of microalgae. The method comprises the steps that (A) a culture medium is prepared, wherein the culture medium is determined according to a target alga plant and sterilized; (B) activation and planting expanding are conducted on the target alga plant, wherein activation and planting expanding are conducted on the target alga plants in a photobioreactor; (C) the target alga plants are inoculated, wherein alga liquid is centrifuged after the inoculation amount is determined, complete washing is performed by repeatedly using the carbon-free culture medium, then resuspension is performed, and a twelve-hole plate is inoculated; (D) OD680 detection is performed; (E) growth force of the target alga plants is evaluated through OD-d-1 and deltaOD-d-1; (F) the alga plants are screened, wherein the distribution of the alga plants in a scatter diagram is determined by adopting a scatter diagram method according to the OD-d-1 and deltaOD-d-1. The device comprises an LED lamp tube, a light-transmitting culture vat, a light-transmitting pore plate, moisture-preserving wavy sponge, a filter, a ventilating guide tube, a six-way gas distributing device, a punching rubber plug, a glass tube, a light-transmitting glass tube and a six-hole frame. The method is simple, easy to operate, simple in operate, quick and convenient and can be applied to screening of fine algae resistant to high concentration of carbon dioxide.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically to a method for rapidly evaluating the tolerance of microalgae to high-concentration carbon dioxide, to a device for evaluating the tolerance of microalgae to carbon dioxide, and to a device for rapid expansion of microalgae. The small-scale photobioreactor device cultivated is especially suitable for screening economical microalgae that can be cultivated by flue gas with large-scale production potential. Background technique [0002] Microalgae are prokaryotic or eukaryotic photosynthetic microorganisms. They are very efficient solar energy converters. They are distributed in fresh water or salt water. They accumulate biomass by absorbing light energy, water, carbon dioxide, and nutrients transmitted by the water environment, and can convert light energy into For chemical energy, accumulate metabolites or secondary metabolites that can be used by humans, such as primary metabolites...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/569G01N2333/405
Inventor 宋立荣卢哲崔慧君卫晴霍岩杨子寒
Owner INST OF AQUATIC LIFE ACAD SINICA