The seedling raising method of gentian flower and the planting method of gentian flower
A technology of gentian flowers and seeds, applied in horticultural methods, planting substrates, botanical equipment and methods, etc., can solve the problems of artificial seedling raising, difficult cultivation, unsatisfactory gentian flower yield, and harsh growth conditions, etc. Achieve the effect of increasing the emergence rate and survival rate, increasing the survival rate, and reducing the amount of picking
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Embodiment 1
[0040] Put the gentian flower seeds into a PVC plastic bottle, and put a small amount of stones in the above PVC plastic bottle, then inject a mass concentration of 10% sodium hypochlorite solution, close the bottle cap tightly, soak and shake for 10 minutes, remove the sodium hypochlorite solution, Pour the seeds in the bottle into a mesh sieve, rinse with sterile water, press the seeds on the mesh sieve while rinsing, and rub the seeds until all the seed coats are removed, and continue to rinse with sterile water for 20 minutes.
[0041] Put the dehulled seeds that have been sucked dry into an ultraviolet sterilizing box, and treat them at a wavelength of 250nm for 3h.
[0042] Peeled seeds were inoculated in the primary culture medium containing 0.1 mg / L indole acetic acid, 0.5 mg / L 6-benzylaminoadenine, 0.05 mg / L EM bacteria and 10 g of perlite with a size of 6 mm. The temperature is 25° C., the light intensity is 2000 lx, the light time is 10 h, and the humidity is 55% to...
Embodiment 2
[0048] Put the gentian flower seeds into a PVC plastic bottle, and put a small amount of stones in the above PVC plastic bottle, then inject a mass concentration of 20% sodium hypochlorite solution, close the bottle cap tightly, soak and shake for 5 minutes, remove the sodium hypochlorite solution, Pour the seeds in the bottle into a mesh sieve, rinse with sterile water, press and rub the seeds on the mesh sieve while rinsing, until all the seed coats are removed, and continue to rinse with sterile water for 30 minutes.
[0049] Put the dehulled seeds that have been sucked dry into an ultraviolet sterilizing box, and treat them at a wavelength of 254nm for 2h.
[0050] Peeled seeds were inoculated in the primary culture medium containing 0.5 mg / L indole acetic acid, 1 mg / L 6-benzylaminoadenine, 0.01 mg / L EM bacteria and 20 g of perlite with a size of 8 mm. 15°C, light intensity of 3000lx, light time of 12h and humidity of 75% for cultivation until sterile seedlings are obtaine...
Embodiment 3
[0056] Put the gentian flower seeds into a PVC plastic bottle, and put a small amount of stones in the above PVC plastic bottle, then inject a mass concentration of 15% sodium hypochlorite solution, close the bottle cap tightly, soak and shake for 6 minutes, remove the sodium hypochlorite solution, Pour the seeds in the bottle into a mesh sieve, rinse with sterile water, press the seeds on the mesh sieve while rinsing, and rub the seeds until all the seed coats are removed, and continue to rinse with sterile water for 25 minutes.
[0057] Put the dehulled seeds that have been sucked dry into an ultraviolet sterilizing box, and treat them at a wavelength of 252nm for 2.5h.
[0058] The dehulled seeds were inoculated in the first-generation culture medium containing 0.3mg / L indole acetic acid, 0.8mg / L 6-benzylaminoadenine, 0.03mg / L EM bacteria and 15g of perlite with a size of 7mm. The temperature is 20° C., the light intensity is 2500 lx, the light time is 11 hours, and the hum...
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