Rapid proliferation method for intermittently immersed Lipu taro tissue culture seedling and Lipu taro tissue culture seedling
An immersion-type technology of Lipu taro, applied in the field of rapid proliferation of Lipu taro seedlings and intermittent submerged Lipu taro tissue culture seedlings, can solve the problems of many seedling raising steps, low degree of mechanization, and long time consumption, and achieve simplified grouping The effect of cultivating operation procedures, preventing accumulation, improving culture quality and reproduction coefficient
Active Publication Date: 2017-05-31
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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Problems solved by technology
[0006] (1) A large amount of manual labor is required in the operation process of the traditional tissue culture method, which is a labor-intensive technology with low degree of mechanization and high cultivation cost;
[0007] (2) the multiplication coefficient of Lipu taro in the prior art is generally 5 times, and the efficiency is very low;
[0008] (3) In the prior art, after the tissue-cultured seedlings of Lipu taro take root, they need to be transferred to the natural environment for hardening treatment for 15-20 days before they can be transplanted into the nutrition cup. Seedling time is also difficult to control
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Embodiment 1
[0069] The specific steps of the method for rapid proliferation of intermittent submerged Lipu taro tissue culture seedlings provided by the present invention are as follows:
[0070] (1) Establishment of the intermittent immersion liquid culture system (TIBs system): the culture bottle and the liquid storage bottle are wide-mouthed white glass bottles with a volume of 3L, the height of the bottle is 30cm, and the diameter is 15cm. The volume of the liquid medium in the liquid storage bottle is 1L / bottle After the medium configuration is completed, the whole set of equipment is sterilized by moist heat sterilization and then used for standby.
[0071] (2) Material selection: the third or fourth generation tissue culture seedlings cultivated by the shoot tip meristem of Lipu taro are used as raw materials;
[0072] (3) Preparation of explants: Cut the adventitious buds grown by the raw material tissue culture seedlings into single buds or double buds during the induction proces...
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The invention provides a rapid proliferation method for an intermittently immersed Lipu taro tissue culture seedling and the Lipu taro tissue culture seedling. According to the method provided by the invention, the Lipu taro tissue culture seedling is cultured by using an intermittently immersed liquid culture system, so that the quality and the propagation coefficient of the culture can be effectively improved. Meanwhile, according to the method provided by the invention, hardening-seedling is not needed, so that the culturing operation program is simplified, the production period of the tissue culture seedling is shortened, and the culture efficiency is high. The Lipu taro tissue culture seedling provided by the invention is cultured by the method provided by the invention and has the advantages of being high in transplanting survival rate and the like.
Description
technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for rapid multiplication of tissue-cultured Lipu taro seedlings of intermittent immersion type and Lipu taro seedlings. Background technique [0002] Taro [Colocasia esculenta (L.) Schott], also known as taro, taro, and hairy taro, is a perennial monocotyledonous herbaceous plant belonging to the genus Colocasia Schott of the family Araceae. Taro is an important vegetable and medicinal plant, which has a long history of cultivation in my country. At the same time, taro is one of the most salt-tolerant vegetable crops found so far. Long-term cultivation can reduce the degree of soil salinization, and has great application value for the development and utilization of tidal flats. Africa has the largest taro planting area in the world, accounting for 79.9% of the total taro planting area, and Asia accounts for 7.6%, mainly concentrated in China, Japan, the Philippines, ...
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 董伟清何芳练颜梅新江文韦绍龙杨柳胡祥红邱祖杨高美萍何青石周运贵杨盈欢
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI