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Quick propagating method of tissue culture seedlings of anoectochilus formosanus

A fast technology for tissue culture seedlings, applied in the field of plant propagation, to achieve the effect of maintaining excellent seed quality, low cost, and improving use value and safety

Inactive Publication Date: 2017-05-31
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because clematis is widely used clinically, and has excellent curative effect, and has extremely high economic and social benefits, so the market demand is increasing, and artificially planted clematis is far from meeting the demand of the market. The price is expensive. The current price of dry products made of clematis is 10,000 yuan per kilogram, and the price of fresh clematis in the market is also as high as 800-1000 yuan per kilogram. If more than 300 kilograms of fresh products can be produced per mu, The output value per mu can reach more than 240,000 yuan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Materials: Anoectochilus roxburghii (wall) lindl.'tianhu') is a healthy strain with strong growth, thick stems, large leaves, clear and many leaf patterns, stress resistance, and strong resistance to diseases and insect pests.

[0025] 2. Drug purchase

[0026] Purchase all conventional medicines in the medium components required for the production of plant tissue culture seedlings, and store the medicines in a dry and cool room for later use.

[0027] 3. Mother liquor preparation

[0028] According to the requirements of the medium formula, the mother liquor is prepared according to the composition of the categories. The medicines are completely dissolved one by one, poured into the selected volumetric flask, and finally made to volume. In case of heating to dissolve the situation, need to wait for the solution to cool down and then make up the volume. The reagent bottle containing the mother solution should be clearly labeled with the name, concentration and pre...

Embodiment 2

[0044] 1. Materials: Anoectochilus roxburghii (wall) lindl.'tianhu') is a healthy strain with strong growth, thick stems, large leaves, clear and many leaf patterns, stress resistance, and strong resistance to diseases and insect pests.

[0045] 2. Drug purchase

[0046] Purchase all conventional medicines in the medium components required for the production of plant tissue culture seedlings, and store the medicines in a dry and cool room for later use.

[0047] 3. Mother liquor preparation

[0048] According to the requirements of the medium formula, the mother liquor is prepared according to the composition of the categories. The medicines are completely dissolved one by one, poured into the selected volumetric flask, and finally made to volume. In case of heating to dissolve the situation, need to wait for the solution to cool down and then make up the volume. The reagent bottle containing the mother solution should be clearly labeled with the name, concentration and pre...

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PUM

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Abstract

The invention discloses a quick propagating method of tissue culture seedlings of anoectochilus formosanus. According to the quick propagating method, superior strains of Tianhu anoectochilus formosanus from Guangdong, Conghua District are used as explants for performing asexual clone and quickly propagating seedlings, thereby ensuring that the seedlings maintain the inherent superior seed nature and medical effect; by using a culture mode of crosswise placement of culture vessels and completely natural light, the problem that the pollution rate is usually higher than 15% in tissue culture and quick propagation of anoectochilus formosanus is effectively solved; and simultaneously, a fluorescent lamp is not used as a light source, so the production cost is obviously reduced and the economic benefit is more obvious. The method disclosed by the invention has the characteristics of low cost, strong seed nature and high medical effect, improves the use safety of the anoectochilus formosanus seedlings, has great importance on resource regeneration and continuous utilization of the endangered medicinal plant anoectochilus formosanus in shortage, and meets the great market demand of the health industry for the precious traditional Chinese medicine anoectochilus formosanus.

Description

[0001] Technical field: [0002] The invention belongs to the field of plant propagation, and in particular relates to a rapid propagation method for tissue-cultured seedlings of A. [0003] Background technique: [0004] Anoectochilus, also known as golden clematis or golden silk grass, is a rare herbaceous perennial plant of the genus Anoectochilus Bl. in the family Orchidaceae. There are about 23 species in my country, and some of them are used for folk medicine. , is a traditional rare and precious Chinese medicinal material. It is widely used in the folk, and it is mostly used to treat diabetes, hyperlipidemia, hepatitis B and other diseases. " and other laudatory titles, known as "the king of medicine". Over the years, due to its large market demand, artificial over-excavation, coupled with the low natural reproduction rate and slow growth of clematis, wild clematis resources have become very scarce and are on the verge of extinction. As early as 1987, the State Council pu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴坤林郑枫曾宋君张建霞张新华马国华段俊
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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