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Culture medium for tissue culture of limonium aureum

A technology of tissue culture and pine pine, which is applied in the field of plant biology, can solve the problems of easy browning and difficult rooting, and achieve the effect of reducing the degradation rate, induction rate and high rooting rate

Inactive Publication Date: 2017-05-31
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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Problems solved by technology

[0003] In recent years, there have been reports in the literature on the tissue culture of Pine japonica, but the explants of the tissue culture of pine pine reported in the literature mostly select seeds, cotyledons or stem segments, and the culture medium mostly uses the improved MS culture medium, this tissue culture method is very prone to browning and is not easy to take root

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  • Culture medium for tissue culture of limonium aureum
  • Culture medium for tissue culture of limonium aureum
  • Culture medium for tissue culture of limonium aureum

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Embodiment Construction

[0017] Statistical calculation formula in the embodiment

[0018] Survival rate = (number of viable explants / total number of inoculated explants) × 100%

[0019] Primary bud induction rate=(the number of explants that germinated / the total number of inoculated explants)×100%

[0020] Subculture multiplier = (the number of clustered buds induced / the total number of buds inserted into a single plant) × 100%

[0021] Rooting rate=(number of explants of differentiated roots / total number of inoculated explants)×100%

[0022] (1) selection and sterilization of explants, take flower buds that will open 2-3 days before flowering, rinse with tap water for 30-40min, and rinse in 70-75% alcohol for 1-2min after absorbing water with absorbent paper, Then treat with 0.1% mercuric chloride solution for 8-10 minutes, rinse with sterile water for 6-7 times, blot dry with sterilized filter paper, and inoculate in improved B5 medium.

[0023] Effects of Explants on the Survival Rate and Induc...

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Abstract

The invention discloses a culture medium for tissue culture of limonium aureum. The culture medium comprises a primary induction medium, a subculture multiplication medium and a rooting culture medium, wherein the formula of the primary induction medium comprises B5+KT of 0.2-0.8mg / L, NAA of 0.05-0.1mg / L, and 2,4-D of 0.02-0.08mg / L; the formula of the subculture multiplication medium comprises B5+KT of 0.5-0.8mg / L and NAA of 0.05-0.1mg / L; and the formula of the rooting culture medium comprises B5+IBA of 0.1mg / L. The tissue culture medium provided by the invention enables the culture reproduction not to be restricted by natural conditions, has a high induction rate and a high rooting rate, can continue to multiply in a large number, solves the problem of less cultivated provenance during culture, can effectively reduce the degradation rate of varieties, provides a guarantee for future research and large-scale and industrial production, provides a technical support for great popularization of the species in landscape construction, and also provides a new scientific basis for species conservation in Northwest China and rapid updating of resources.

Description

technical field [0001] The invention belongs to the field of plant biotechnology and relates to a tissue culture medium, in particular to a culture medium for tissue culture of pine pine Background technique [0002] Limonium au-reum, also known as golden limonium and limonium limonium, is a perennial herb. The yellow-flowered rock pine belongs to the genus Limonium genus of Orchidaceae. It is one of the few wild flowers in the arid desert area. It has bright colors and a long flowering period. The root is a taproot system, thick and smooth, the whole plant is smooth, the plant height is 20-50cm, extensive management, extremely drought-resistant, long flowering period, it is an important germplasm resource for enriching the ground cover plant varieties in the greening construction of northern cities, and it is also windproof and sand-fixing. fine plants. The yellow-flowered rocky pine is very resistant to drought and high temperature, salt and alkali, and barrenness. In th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 马怀林张永清刘思泱崔海鹏智磊白俊梅云娜李晶晶王召明高秀梅田志来李德奎徐沁贞杨阳
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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