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Production process of sheep placenta extract

A production process and technology for extracting liquid, applied in the field of sheep placenta extract, can solve the problems of large amount of enzyme, waste of resources, complex extraction process and the like

Active Publication Date: 2017-05-31
HUZHOU ZHENLU BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing extraction process of sheep placenta extract is relatively complicated, and the amount of enzyme used in the extraction process is relatively large, resulting in a large extraction cost
The extraction rate of sheep placenta extract is generally about 50%, and the utilization efficiency of sheep placenta is not high, resulting in a great waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The following production process is adopted: S1, choose fresh sheep placenta, remove impurities and put it in a stainless steel bucket for 1-2 hours; S2, wash it with clean water for 3-5 times after soaking, and then put it in a centrifugal dehydrator for 1-2 hours. 2 times; S3, crush the dehydrated sheep placenta in a meat grinder; S4, put the crushed sheep placenta into the reaction kettle, add distilled water, adjust the pH to 8.0-8.5, and then put the reaction kettle Put it in an electric constant temperature water bath for heating for 10-20 minutes, cool and keep warm to 40°C-50°C, add 0.5% trypsin, and after 3-5 hours of enzymolysis, raise the temperature to 70°C-80°C to kill trypsin; cool and keep warm to 50°C to 60°C, adjust the pH to 7.0, add pronase neutral, enzymatically hydrolyze for 5 to 8 hours, then raise the temperature to 100°C to 110°C to kill the pronase neutral, and carry out sterilization treatment at the same time; S5. After sterilizing and deactiv...

Embodiment 2

[0015] The following production process is adopted: S1, choose fresh sheep placenta, remove impurities and put it in a stainless steel bucket for 1-2 hours; S2, wash it with clean water for 3-5 times after soaking, and then put it in a centrifugal dehydrator for 1-2 hours. 2 times; S3, crush the dehydrated sheep placenta in a meat grinder; S4, put the crushed sheep placenta into the reaction kettle, add distilled water, adjust the pH to 8.0-8.5, and then put the reaction kettle Put it into an ultrasonic water bath heating pot for heating for 10-20 minutes, cool and keep warm to 40°C-50°C, add 0.5% trypsin, and after 3-5 hours of enzymolysis, heat up to 70°C-80°C to kill trypsin; cool and keep warm to 50°C-60°C, adjust the pH to 7.0, add pronase neutral, enzymatically hydrolyze for 5-8 hours, then raise the temperature to 100°C-110°C to kill the pronase neutral, and carry out sterilization treatment at the same time; S5, Cool the sterilized and enzyme-killed sheep placenta solu...

Embodiment 3

[0017] The following production process is adopted: S1, choose fresh sheep placenta, remove impurities and put it in a stainless steel bucket for 1-2 hours; S2, wash it with clean water for 3-5 times after soaking, and then put it in a centrifugal dehydrator for 1-2 hours. 2 times; S3, crush the dehydrated sheep placenta in a meat grinder; S4, put the crushed sheep placenta into the reaction kettle, add distilled water, adjust the pH to 8.0-8.5, and then put the reaction kettle Put it in an electric constant temperature water bath for heating for 10-20min, cool and keep warm to 40℃-50℃, add 0.5% trypsin and 1.2% Tween-20, enzymolysis for 3-5 hours, then heat up to 70℃-80℃ Inactivate trypsin; cool and keep warm to 50°C-60°C, adjust the pH to 7.0, add neutral streptavidin and polyethylene glycol, enzymatically hydrolyze for 5-8 hours, then raise the temperature to 100°C-110°C to kill neutral streptavidin Protease, and sterilize at the same time; S5, cool the sterilized and enzym...

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Abstract

The invention relates to the technical field of sheep placenta extract, and in particular relates to a production process of sheep placenta extract. The production process comprises the following production steps of: S1, soaking fresh sheep placenta for 1-2 hours; S2, cleaning the soaked sheep placenta by clear water for 3-5 times, and putting the cleaned sheep placenta into a centrifugal dehydrator to carry out dehydration for once to twice; S3, putting the sheep placenta into a meat grinder to carry out grinding; S4, carrying out enzymatic hydrolysis for twice under the premise of ultrasonic heating; S5, cooling the sterilized and enzyme deactivated sheep placenta to normal temperature, pouring the sheep placenta into a stainless steel barrel, adding 2% of active carbon and carry out heating for 3-5 minutes; and S6, adding 3-5% of ethyl alcohol and 0.05-0.08% of ammonium sulfide, carrying out heating for 20-30 minutes, cooling to normal temperature and carrying out filtration to obtain the sheep placenta extract. The production process is simple; and through the production process, the usage amount of the enzyme is decreased, and the enzymolysis efficiency is improved through the cooperation of an ultrasonic wave and a surfactant, so that the extraction efficiency of the sheep placenta extract is improved.

Description

technical field [0001] The invention relates to the technical field of sheep placenta extract, in particular to a production process of sheep placenta extract. Background technique [0002] "Placenta" is a commonly used health care product, and it is a general term for small molecule compounds with immune activity extracted from placenta, also known as "placental peptide". According to the theory of traditional Chinese medicine, the placenta is dry, salty, and returns to the lung and kidney channels. The function is to invigorate qi, nourish blood, and benefit essence. It can be used for nourishing, beautifying, and strengthening. Its efficacy mainly has the following points: it has a health care effect on the skin, has a great influence on immunity and exercise ability, anti-fatigue effect, anti-aging effect, and cellular immunity effect. Because the placenta has the above-mentioned many functions, it has become an important subject to use modern bioengineering technology...

Claims

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Application Information

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IPC IPC(8): A61K35/50A61P1/14A61P39/00A61P39/06A23L33/10
CPCA23V2002/00A61K35/50A23V2200/302A23V2200/324A23V2250/204
Inventor 沈志泉蔡六珠
Owner HUZHOU ZHENLU BIOLOGICAL PROD
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