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Cordyceps militaris extract and preparation method and application thereof

A technology of Cordyceps militaris and its extract, applied in the field of natural active products, can solve the problems of poor druggability and achieve low toxicity and high safety

Active Publication Date: 2017-05-31
BEIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are many PTP1B inhibitors, but most of them are artificially synthesized, have great side effects, and have poor clinical druggability. So far, no PTP1B inhibitor has been clinically applied

Method used

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  • Cordyceps militaris extract and preparation method and application thereof
  • Cordyceps militaris extract and preparation method and application thereof
  • Cordyceps militaris extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Cordyceps militaris extract is prepared by the following method:

[0055] 1. Preparation of alcohol extract.

[0056] Take 200g of Cordyceps militaris coarse powder, add 2000ml of 95% ethanol aqueous solution at a solid-liquid ratio of 1:10 (10ml of solution per gram of raw material solid), reflux in a water bath at 80°C for 2h, 4 times in total, filter, and obtain alcohol Extract the solution and combine the alcohol extract; concentrate under reduced pressure to recover ethanol, combine the concentrate, and continue to heat and dry at 55°C in an open container to obtain the alcohol extract, weighing 35.4g.

[0057] 2. Preparation of cyclohexane soluble matter.

[0058] Put the above-mentioned ethanol extract in a larger container, add 1770ml (50 times) cyclohexane, stir, stand still, filter, collect the cyclohexane solution, repeat this process 4 times, and concentrate the cyclohexane solution under reduced pressure to recover Cyclohexane, combined concentrated solut...

Embodiment 2

[0079] Cordyceps militaris extract is prepared by the following method:

[0080] 1. Preparation of alcohol extract.

[0081] Take 500g of Cordyceps militaris coarse powder, add 7500ml of absolute ethanol at a solid-to-liquid ratio of 1:15 (15ml of solution per gram of raw material solid), reflux in a water bath at 80°C for 1.5h, 4 times in total, filter to obtain an alcohol extract, combine the alcohol Extraction: Concentrate under reduced pressure to recover ethanol, combine the concentrated solution, and continue to heat and dry in an open container at 55°C to obtain the alcohol extract, weighing 90g.

[0082] 2. Preparation of cyclohexane soluble matter.

[0083] Put the above ethanol extract in a larger container, add 5.4L (60 times) cyclohexane, stir, stand still, filter, collect the cyclohexane solution, repeat this process 5 times, and concentrate the cyclohexane solution under reduced pressure The cyclohexane was recovered, the concentrated solution was combined, and...

experiment example 1

[0097] Get the ethyl acetate soluble matter prepared in Example 1, n-butanol alcohol soluble matter, component 3, component 4, component 5, component 6, component 7, monomeric compound 1 and monomeric compound 2, The components were tested for their inhibitory effect on protein tyrosine phosphatase 1B.

[0098] 1. Materials

[0099] 1. Take ethyl acetate solubles, n-butanol solubles, component 3, component 4, component 5, component 6, and component 7, and make them into a concentration of 0.5 mg / mL;

[0100] 2. Monomer compound 1 and monomer compound 2;

[0101] 3. PTP1B, purchased from Prospec;

[0102] 4. Tris, purchased from the background of the limited liability company Dingguo Biotechnology Co., Ltd.;

[0103] 5. HCl, purchased from Xilong Chemical Co., Ltd.;

[0104] 6. P-NPP, purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd.;

[0105] 7. Ursolic acid and corosolic acid were purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd., and the ...

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Abstract

The invention relates to a cordyceps militaris extract and a preparation method and application thereof and belongs to the technical field of natural active products. The preparation method includes the steps of preparing alcohol extract, to be specific, weighing cordyceps militaris, adding ethanol water with a volume percentage concentration of 80-100%, as extraction solvent, into the cordyceps militaris, extracting while heating, removing insoluble substance to obtain alcohol extract liquid, recycling the solvent in the alcohol extract liquid to obtain the alcohol extract, namely the cordyceps militaris extract. The cordyceps militaris extract prepared by the method has inhibitional effect to protein-tyrosine-phosphatase 1B (PTP1B), can be applied to developing and preparing drugs for reducing blood glucose, preventing insulin resistance and treating diabetes mellitus.

Description

technical field [0001] The invention relates to the technical field of natural active products, in particular to an extract of Cordyceps militaris and its preparation method and application. Background technique [0002] Diabetes mellitus (DM) is a chronic metabolic disease characterized by hyperglycemia. In recent years, with the continuous progress of society and the continuous improvement of people's living standards, the incidence of diabetes has also been continuously increased. Diabetes has become one of the malignant diseases that seriously endanger human life and health. Clinically, hyperglycemia is the main feature. The overall prevalence of diabetes in my country has reached 9.7%, and the prevalence of prediabetes is as high as 15.5% in the same period. Estimates based on the survey results show that the total number of people with diabetes in my country is more than 92 million, and the number of people with pre-diabetes is more than 148 million. [0003] Recent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/068A61P3/10A61P5/50A23L33/00
CPCA61K31/7032A61K36/068C07H1/08C07H15/10
Inventor 孙晶波杜培革安丽萍苑广信赵南晰韩笑徐广宇李洪宇郭笑盛瑜王曼力侯志东
Owner BEIHUA UNIV
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