Preparation method of polydopamine microcapsule oral insulin administration carrier

A technology of polydopamine microcapsules and drug delivery carriers is applied in the directions of pharmaceutical formulations, medical preparations without active ingredients, and medical preparations containing active ingredients, etc. The effect of good capacitance and long residence time

Inactive Publication Date: 2017-05-31
XI'AN PETROLEUM UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above analysis results show that it is difficult to prepare an oral insulin delivery carrier with good biocompatibility and the ability to overcome the gastrointestinal barrier through a mild, rapid and controllable reaction. Come new development opportunities

Method used

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  • Preparation method of polydopamine microcapsule oral insulin administration carrier
  • Preparation method of polydopamine microcapsule oral insulin administration carrier
  • Preparation method of polydopamine microcapsule oral insulin administration carrier

Examples

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Effect test

Embodiment 1

[0032] Embodiment 1, a preparation method of a polydopamine microcapsule oral insulin delivery carrier, comprising the following steps:

[0033] 1) Dissolve the insulin powder with a hydrochloric acid solution with a pH of 2 to a concentration of 10 mg / mL, add sodium chloride crystals thereinto until the amount of sodium chloride reaches 0.6 mol / L, and react for 1 h under shaking in a water bath at 15°C. It was observed that white insulin particles were gradually precipitated, and the product was centrifuged, and the product was washed twice with 2mol / L sodium chloride solution, and centrifuged to obtain insulin micro-nano particles;

[0034]2) First, prepare a Tris-HCl buffer solution with a pH of 7.5; secondly, disperse the insulin micronanoparticles in the Tris-HCl buffer solution so that the concentration of insulin is 10 mg / mL, add dopamine powder to make the concentration 2 mg / mL, The above reaction system was placed at room temperature and reacted at a speed of 900 rpm ...

Embodiment 2

[0043] Embodiment 2, a preparation method of a polydopamine microcapsule oral insulin delivery carrier, comprising the following steps:

[0044] 1) Dissolve the insulin powder with a hydrochloric acid solution with a pH of 1 to a concentration of 50 mg / mL; add sodium chloride crystals to it until the amount of sodium chloride reaches 0.5 mol / L; react for 3 hours under shaking in a water bath at 5°C, It was observed that white insulin particles were gradually precipitated; centrifuged, washed twice with 2mol / L sodium chloride solution, and centrifuged to obtain insulin micro-nano particles;

[0045] 2) Prepare a Tris-HCl buffer solution with a pH of 7.5, disperse the insulin micronanoparticles in the Tris-HCl buffer solution so that the concentration of insulin is 80 mg / mL, add dopamine powder to make the concentration 2 mg / mL; the above reaction system Place at room temperature and react at a speed of 900 rpm for 24 hours. During the reaction, the color of the solution gradual...

Embodiment 3

[0047] Embodiment 3, a preparation method of polydopamine microcapsule oral insulin delivery carrier, comprising the following steps:

[0048] 1) Use hydrochloric acid solution with a pH of 1.5 to dissolve the insulin powder to a concentration of 20 mg / mL; add sodium chloride crystals to it until the amount of sodium chloride reaches 2 mol / L; react for 1 hour under shaking in a water bath at 15°C, and observe The white insulin particles are gradually precipitated; centrifuged, washed twice with 2mol / L sodium chloride solution, and centrifuged to obtain insulin micro-nano particles;

[0049] 2) Prepare a Tris-HCl buffer solution with a pH of 8.5, disperse the upper insulin micro-nanoparticles in the Tris-HCl buffer solution so that the concentration of insulin is 40 mg / mL, add dopamine powder to make the concentration 2 mg / mL; The system was placed at room temperature and reacted at a speed of 900 rpm for 24 hours; during the reaction, the color of the solution gradually change...

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Abstract

The invention relates to a preparation method of a polydopamine microcapsule oral insulin administration carrier. The preparation method comprises the following steps: dissolving insulin powder in an acidic solution, adding sodium chloride, and preparing insulin micro/nano particles by a salting-out process; dispersing the insulin micro/nano particles in a dopamine weakly-alkaline solution, and reacting under oscillation conditions to obtain a polydopamine-coated insulin core-shell structure; and finally, dispersing the polydopamine-coated insulin core-shell structure in a functional molecule solution, and modifying functional molecules on the surface, thereby obtaining the biocompatible polydopamine microcapsule oral insulin administration carrier capable of overcoming the intestinal tract barrier. The polydopamine microcapsule oral insulin administration carrier has the p-responsive insulin release behavior, has the advantages of favorable cell compatibility and the like, can overcome the intestinal tract barrier, and can be used for treating diabetes.

Description

technical field [0001] The invention relates to the technical field of insulin delivery carrier, in particular to a preparation method of polydopamine microcapsule oral insulin delivery carrier. Background technique [0002] Insulin is the most commonly used and most effective drug for treating diabetes. Currently, the most commonly used method of administering insulin is subcutaneous injection. This method of administration is prone to infection, allergic reactions, and lipodystrophy at the injection site, and the shortcomings of injection pain and the need for special personnel to administer the drug also bring great troubles to patients. Therefore, there is an urgent need to research and develop new insulin delivery systems that are more economical, convenient and painless. [0003] Oral insulin delivery system is a new type of insulin delivery system developed in recent years. The physiological mechanism of this system is similar to the endogenous pathway of insulin af...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/28A61K47/34A61K47/36A61K47/42A61P3/10
CPCA61K38/28A61K47/34A61K47/36A61K47/42A61K9/0053
Inventor 李红彭浩南燕永利陈刚顾雪凡
Owner XI'AN PETROLEUM UNIVERSITY
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