A subway engineering concrete containing microbial capsules capable of continuous self-healing
A microbial and concrete technology, applied in the direction of being fixed on or in an inorganic carrier, or on or in an organic carrier, can solve the problems of strength effect, poor adhesion, easy to be washed away, etc., to achieve good resistance The effect of compressive strength, large adsorption capacity and strong adhesion
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Embodiment 1
[0027] Prepare microbial capsules:
[0028] Step 1: Cultivate Bacillus lysinus, and obtain the sludge after centrifugation, the concentration of the sludge is 6×10 9 a / ml;
[0029] Step 2, configure anhydrous Bacillus lysinus, tryptone 510g, soybean peptone 90g, glucose 75, NaCl 150g; dipotassium hydrogen phosphate 25g;
[0030] Step 3. Add all the medium and 0.1ml of bacteria sludge prepared in step 2 into 1200ml of water and stir evenly to obtain a mixed solution;
[0031] Step 4. After adding 100 g of macroporous adsorption resin to the mixed solution obtained in step 3, stir at a speed of 250 r / min for 30 minutes, filter and collect the macroporous adsorption resin, and dry at 20°C until the water content is lower than 5%;
[0032] Step 5. Place the dried macroporous adsorbent resin in 90%wt alcohol for the first soaking for 30 minutes, and then evenly spray 20% cement liquid on its surface to make the thickness 0.3mm. Dry at ℃, spray again the mixed solution of cement ...
Embodiment 2
[0036] Prepare microbial capsules:
[0037] Step 1. Cultivate Bacillus lysinus or Bacillus cereus, centrifuge to obtain the sludge, the concentration of the sludge is 6×10 9 a / ml;
[0038] Step 2, configure anhydrous aerobic alkalophilic bacillus culture medium, tryptone 510g, soybean peptone 90g, glucose 75, NaCl 150g; dipotassium hydrogen phosphate 25g;
[0039] Step 3. Add all the medium and 0.1ml of bacteria sludge prepared in step 2 into 1500ml of water and stir evenly to obtain a mixed solution;
[0040] Step 4. After adding 100 g of macroporous adsorption resin to the mixed solution obtained in step 3, stir at a speed of 250 r / min for 20 minutes, filter and collect the macroporous adsorption resin, and dry at 30°C until the water content is lower than 5%;
[0041] Step 5. After soaking the dried macroporous adsorption resin in 100%wt alcohol for 50 minutes once, spray the cement liquid with a mass fraction of 25% evenly on the surface to make the thickness 0.8mm, and ...
Embodiment 3
[0044] Prepare microbial capsules:
[0045] Step 1: Cultivate Bacillus cereus, obtain the sludge after centrifugation, and the concentration of the sludge is 4×10 9 a / ml;
[0046] Step 2. Configure anhydrous M.S.P medium, NaNO 3 9g, K 2 HPO 4 3g, KCI1.5g, MgSO 4 1.5g, FeSO 4 , 0.03g, 90g of sucrose;
[0047] Step 3. Add all the medium and 0.1ml of bacteria sludge prepared in step 2 into 180ml of water and stir evenly to obtain a mixed solution;
[0048] Step 4: After adding 15 g of macroporous adsorbent resin to the mixed solution obtained in step 3, stir at a speed of 250 r / min for 30 minutes, filter and collect the macroporous adsorbent resin, and dry at 30°C until the water content is lower than 5%;
[0049] Step 5. After soaking the dried macroporous adsorption resin in 100%wt alcohol for 40min once, spray the cement liquid with a mass fraction of 25% on the surface evenly to make the thickness 0.5mm, and dry it at 30°C Dry in the box, spray again the mixed solutio...
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