Obtaining method and application of microbial flora for fermenting enteromorpha to prepare marsh gas

A technology of microbial flora and Enteromorpha, which is applied in the field of obtaining salt-tolerant microbial flora, can solve problems such as unachievable process, difficult industrial production, and insufficient sea salt tolerance, and achieve high stability and degradation efficiency. Obtain the effect of simple method and high salt tolerance

Inactive Publication Date: 2017-05-31
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patents CN101418315A, CN 102876727A, and CN 104642530A provide a variety of methods for producing biogas using Enteromorpha as raw materials, but these methods need to use clean water to remove the salt in Enteromorpha or prepare fermentation broth with fresh water, because Enteromorpha belongs to the ocean The algae after harvesting Enteromorpha carry a large amount of sea salt, while the fermentation flora used in the previous technology is usually enriched from lake slud

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Gained bacterium liquid is added in fermenter, and its add-on is 40% of fermentor volume, then adds the enteromorpha that does not clean salt in fermenter, and its add-on is 1g / 100mL, regulates system salinity simultaneously to be 10‰, The initial pH value of the system is 6.8; the air in the fermenter is discharged for anaerobic fermentation, the fermentation temperature is 25°C, and the batch fermentation is carried out for 30 days, and the pH value is maintained between 6.5-7.0 during the fermentation process. After the fermentation, the degradation rate of Enteromorpha was 79%, the gas production was 263mL / g, and the methane concentration was 59%.

Embodiment 2

[0034] Gained bacterium liquid is added in fermentor, and its add-on is 80% of fermentor volume, then adds the Enteromorpha enteromorpha that does not clean salt in fermenter, and its add-on is 10g / 100mL, and regulating system salinity is 35‰ simultaneously, The initial pH value of the system is 7.8; the air in the fermenter is exhausted for anaerobic fermentation, the fermentation temperature is 40°C, batch fermentation is carried out for 15 days, and the pH value is maintained between 7.5-8.0 during the fermentation process. After the fermentation, the degradation rate of Enteromorpha was 75%, the gas production was 258 mL / g, and the methane concentration was 56.5%.

Embodiment 3

[0036] Gained bacterium liquid is added in fermentor, and its add-on is 60% of fermentor volume, then in fermentor, adds the Enteromorpha enteromorpha that does not clean salt, and its add-on is 5g / 100mL, and regulating system salinity is 20‰ simultaneously, The initial pH value of the system is 7.2; the air in the fermenter is exhausted for anaerobic fermentation, the fermentation temperature is 30°C, and the continuous fermentation should be fed again when the daily gas production is lower than 20% of the daily average gas production; the pH is maintained during the fermentation process The value is between 6.8-7.5. After the fermentation, the degradation rate of Enteromorpha was 78%, the gas production was 277 mL / g, and the methane concentration was 62.5%.

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Abstract

The invention discloses an obtaining method and application of microbial flora for fermenting enteromorpha to prepare marsh gas. According to the obtaining method, the enteromorpha is used as a matrix; silt and seawater collected on an intertidal zone are utilized; the microbial flora is obtained through continuous fermentation and domestication. The flora obtained by the obtaining method can maintain favorable activity and a higher gas yield in the sweater of which the salinity is 1 percent to 2 percent, and degrades the enteromorpha in a system of which the salinity is 3.5 percent or below to generate the marsh gas; in the condition that an enteromorpha initial material is not washed by using fresh water, the high-efficiency degradation on the enteromorpha can be realized; the purpose of saving a water resource and a time are achieved; biological fuel gas can be prepared while the harm caused to an offshore oceanic environment by the enteromorpha is deceased; the obtaining method has favorable economic benefit and environmental benefit.

Description

technical field [0001] The invention belongs to the technical field of microbes and bioenergy, and in particular relates to a method for obtaining salt-tolerant microbial flora used for fermenting enteromorpha to prepare biogas and its application. Background technique [0002] Enteromorpha( Enteromorph ) is a large green algae belonging to Chlorophyceae and Ulvaceae. The common species include Enteromorpha enteromorpha, Enteromorpha flatus, Enteromorpha marginatus, Enteromorpha barbata, etc. Enteromorpha has tenacious vitality, high environmental adaptability, and fast reproduction speed. Therefore, its large-scale growth will have a strong inhibitory effect on the growth of other algae in the same sea area. In recent years, due to the aggravation of marine environmental pollution and eutrophication of seawater, the vicious reproduction of Enteromorpha has been caused. At the same time, Enteromorpha floats to the coast to rot and stink, not only causing great harm to the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12P5/02C12R1/01
CPCC12N1/02C12N1/20C12P5/023Y02E50/30
Inventor 赵超艾超严新林雅静刘斌肖正
Owner FUJIAN AGRI & FORESTRY UNIV
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