Preparation method of SERS (surface enhanced Raman spectrum) substrate and sample

A surface-enhanced Raman and spectroscopy technology, applied in the field of chemical analysis, can solve the problems of low signal uniformity of SERS substrate, complex preparation process of SERS substrate, expensive SERS substrate, etc., and achieve good repeatability between samples and short sample preparation time Shortened, regularized effect

Inactive Publication Date: 2017-05-31
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] In summary, the signal uniformity of SERS substrates is usually not high, and the preparation process of SERS substrates with high uniformity is complicated, the technical requirements are high, and the commercial SERS substrates are expensive. These problems greatly limit the use of SERS technology in the laboratory. Or the application in the actual detection

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  • Preparation method of SERS (surface enhanced Raman spectrum) substrate and sample
  • Preparation method of SERS (surface enhanced Raman spectrum) substrate and sample
  • Preparation method of SERS (surface enhanced Raman spectrum) substrate and sample

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preparation example Construction

[0033] The invention provides a method for preparing a surface-enhanced Raman spectrum substrate, comprising the following steps:

[0034] (1) mixing metal nanoparticles with water to obtain a metal nanoparticle dispersion, the metal nanoparticles comprising gold nanoparticles and / or silver nanoparticles;

[0035] (2) Put the metal nanoparticle dispersion obtained in the step (1) on the surface of the filter membrane, and vacuum filter to obtain a surface-enhanced Raman spectrum substrate.

[0036] The invention mixes the metal nano particles with water to obtain the metal nano particle dispersion liquid, and the metal nano particles include gold nanoparticles or silver nanoparticles. In the present invention, there is no special limitation on the preparation operation of the metal nanoparticle dispersion, and the technical solution for the preparation of the dispersion well known to those skilled in the art can be used.

[0037] In the present invention, the metal nanopartic...

Embodiment 1

[0059] (1) Preparation of noble metal nanoparticles

[0060] Take by weighing 4.84mL mass concentration and be that 1% chloroauric acid solution is diluted to 400mL ultrapure water, be heated to boiling, under the situation of stirring at 1200rpm, add 2.735mL mass concentration rapidly and be 1% trisodium citrate solution, in Keep boiling for 30 min with stirring at 1200 rpm and then cool to room temperature to obtain purple-red Au nanoparticles.

[0061] (2) Cleaning and concentrating metal nanoparticles

[0062] Put the Au nanoparticles prepared in step (1) into a glass centrifuge tube, centrifuge at 2900rpm for 8min, remove the supernatant, and obtain the washed Au nanoparticles; add a certain volume of ultrapure water, and mix well, Centrifuge at 2900 rpm for 6 min, remove the supernatant, and obtain a metal nanoparticle dispersion with a concentration of 8 g / L in the lower layer.

[0063] (3) Preparation of surface-enhanced Raman spectroscopy substrate

[0064] A polys...

Embodiment 2

[0066] Dilute 10 μL to a concentration of 10 -5 The adenine solution of M was dropped on the surface-enhanced Raman spectrum substrate obtained in Example 1, and suction-filtered at -0.01-0.05 MPa for 3 seconds to obtain a surface-enhanced Raman spectrum sample.

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Abstract

The invention provides a preparation method of an SERS (surface enhanced Raman spectrum) substrate and an SERS sample. In the preparation method, a filter membrane is taken as a carrier, rapid drying is realized by vacuum drawing filtration, and the obtained SERS substrate and the obtained SERS sample are uniform in thickness, regular in shape, uniform in SERS signals and high in repeatability. An experimental result indicates that the detection limit of SERS signals of an SERS sample of adenine prepared by the method provided by the invention reaches 10<8> M, the signals have high uniformity at different sampling points on different samples or on a same sample, and the repeatability among the samples is high; and Raman signals of an SERS sample of enterobacterium can be lowered to 3.6% relatively to the standard deviation, and the uniformity and the repeatability are high.

Description

technical field [0001] The invention relates to the technical field of chemical analysis, in particular to a preparation method of a surface-enhanced Raman spectrum substrate and a sample. Background technique [0002] Raman spectroscopy, like infrared spectroscopy, belongs to molecular vibration spectroscopy, which can reflect the characteristic structure of molecules. However, the Raman scattering effect is a very weak process, and generally its light intensity is only about 10 of the incident light intensity. -8 . Therefore, the Raman signals are very weak, and some kind of enhancement effect is almost always used to study the Raman spectroscopy of surface adsorbed species. [0003] Since Surface-enhanced Raman Scattering (SERS) was discovered in 1974, the Raman signal of substances close to or adsorbed on the surface of nanoparticles can be obtained by using the huge electromagnetic field enhancement effect of nanoparticles such as gold and silver. Enhanced by more th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/34
CPCG01N21/658G01N1/34
Inventor 崔丽
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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