Beta 2-microglobulin assay kit and preparation method thereof

Abstract

The invention discloses a beta 2-microglobulin assay kit and a preparation method thereof. The kit comprises a reagent A, a reagent B and a calibration product, wherein the reagent A is prepared from the following raw materials in parts by weight: 25-30 parts of a buffer solution, 20-25 parts of sodium azide, 15-20 parts of a stabilizer and 11-16 parts of a surface activator; the reagent B is prepared from the following raw materials in parts by weight: 23-27 parts of latex particles combined with anti-human beta 2-microglobulin antibody, 21-24 parts of sodium azide and 25-30 parts of the buffer solution; the calibrator is prepared from the following raw materials in parts by weight: 15-18 parts of beta 2-microglobulin, 21-24 parts of the buffer solution and 16-21 parts of the stabilizer; the buffer solution comprises the following raw materials in parts by weight: 17-22 parts of tris(hydroxymethyl) aminomethane, 30-35 parts of deionized water and 11-20 parts of hydrochloric acid. The preparation method for the beta 2-microglobulin assay kit comprises the following steps: mixing tris(hydroxymethyl) aminomethane with deionized water, uniformly stirring, adding hydrochloric acid and regulating the PH value to be 7-9. The beta 2-microglobulin assay kit has excellent sensitivity for analysis, precision degree and accuracy, is relatively great in measurement range, is simple to prepare, is convenient to use, and is low in cost.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a β2-microglobulin assay kit and a preparation method thereof. Background technique [0002] β2 microglobulin is a small molecular globulin produced by lymphocytes, platelets, and polymorphonuclear leukocytes, with a molecular weight of 11,800 and a single-chain polypeptide composed of 99 amino acids; it is a human leukocyte antigen (HLA) on the cell surface Part of the β chain (light chain), containing a pair of disulfide bonds in the molecule, without sugar; similar to the structure of the stable region of immunoglobulin; widely present in plasma, urine, cerebrospinal fluid, saliva and colostrum; normal human β2 - The synthesis rate of microglobulin and the amount released from the cell membrane are quite constant, β2-microglobulin can be freely filtered from the glomerulus, 99.9% is absorbed in the proximal renal tubule, and is decomposed and destroyed in th...

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Problems solved by technology

[0003] The patent document with the application number 201010139410.6 discloses a kit for detecting β2-microglobulin by nano-microsphere immunoturbidimetric method, which is used to provide a kit that does not require dilution, is simple to operate, has high accuracy and good repeatability, and is suitable for various The nano-microsphere immunoturbidimetric assay kit for detecting β2-microglobulin of a type of fully automatic biochemical analyzer, but its analysis sensitivity is low, the measurement range is small, the precision and accuracy are low, and it is inconvenient to use. range is limited, therefore, we propose a β2-microglobulin assay kit and its preparation method