Rapid propagation method for cyclobalanopsis glauca tissue culture
A technology of tissue culture and qinggang tree, which is applied in the field of plant propagation, can solve the problems of slow growth and low bud multiplication coefficient, and achieve the effects of large yield of subculture buds, simple operation process and reduced production cost
Inactive Publication Date: 2017-06-06
唐春艳 +1
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The invention discloses a rapid propagation method for cyclobalanopsis glauca tissue culture. The rapid propagation method comprises the work procedures of selecting explant, processing the explant, inducing and culturing initial buds, multiplication culturing, culturing strong buds and rooting culturing and comprises the steps of collecting underyearling twigs of cyclobalanopsis glauca which is in half lignifications and grows robustly as the explant, pruning and sterilizing the explant, inoculating the explant into an initial bud inducing and culturing medium to obtain the initial buds, then inoculating the initial buds into a multiplication culturing medium, forming cluster buds after 3 to 4 periods of multiplication culture, inoculating the cluster buds into a strong bud culture medium to be cultured and finally inserting single buds with the height larger than or equal to 2cm into a rooting culture medium to be induced and rooted. The rapid propagation method disclosed by the invention shortens a seedling culture period of the cyclobalanopsis glauca, saves seedling culture materials, overcomes the problems that a traditional seedling culture method needs a lot of seedling culture materials and has a long period, greatly reduces production cost, improves production efficiency and has better economical benefit, social benefit and ecological benefit.
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