Rapid propagation method for cyclobalanopsis glauca tissue culture

A technology of tissue culture and qinggang tree, which is applied in the field of plant propagation, can solve the problems of slow growth and low bud multiplication coefficient, and achieve the effects of large yield of subculture buds, simple operation process and reduced production cost

Inactive Publication Date: 2017-06-06
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for tissue culture and propagation of twigs with good growth effect a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024] Example 1:

[0025] (1) Explant selection: collect semi-lignified and robust growing green shoots of the year as explants;

[0026] (2) Explant treatment: Rinse the explants under tap water for 10 minutes, then remove the leaves of the explants, and then soak the explants in a 0.1% mercury solution, and control the soaking time for 15 minutes. Finally, wash the medicine with pure water, cut the explants into 1.5-3cm long stems with at least one axillary bud, place them in containers according to the degree of lignification of the stems, and reserve them;

[0027] (3) Initial bud induction culture: inoculate the explants obtained in step (2) in the initial bud induction medium and cultivate 30-35 in an environment of light culture, 500 lx light, and culture temperature of 20±1℃ d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified WPM medium+IBA 2.0 mg / L+6-BA 1.0 mg / L+ZT 1.5 mg / L+ VC 15 mg / L+Folic acid 10mg / L+VB 2 15 mg / +agar...

Example Embodiment

[0032] Example 2:

[0033] (1) Explant selection: collect semi-lignified and robust growing green shoots of the year as explants;

[0034] (2) Explant treatment: Rinse the explants under running water for 15 minutes, then remove the leaves of the explants, and then soak the explants in a mercury solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into 1.5-3cm long stems with at least one axillary bud, place them in containers according to the degree of lignification of the stems, and reserve them;

[0035] (3) Initial bud induction culture: inoculate the explants obtained in step (2) in the initial bud induction medium and cultivate 30-35 in an environment of light culture, 1000 lx light, and culture temperature of 20±1℃ d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified WPM medium+IBA 3.0 mg / L+6-BA 1.5 mg / L+ZT 1.5mg / L+ VC 15 mg / L...

Example Embodiment

[0040] Example 3:

[0041] (1) Explant selection: collect semi-lignified and robust growing green shoots of the year as explants;

[0042] (2) Explant treatment: Rinse the explants under running water for 15 minutes, then remove the leaves of the explants, and then soak the explants in a mercury solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into 1.5-3cm long stems with at least one axillary bud, place them in containers according to the degree of lignification of the stems, and reserve them;

[0043] (3) Initial bud induction culture: inoculate the explants obtained in step (2) in the initial bud induction medium and cultivate 30-35 in an environment of light culture, 1000 lx light, and culture temperature of 20±1℃ d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified WPM medium+IBA 2.5 mg / L+6-BA 2.0 mg / L+ZT 2.0mg / L+ VC 15 mg / L...

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PUM

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Abstract

The invention discloses a rapid propagation method for cyclobalanopsis glauca tissue culture. The rapid propagation method comprises the work procedures of selecting explant, processing the explant, inducing and culturing initial buds, multiplication culturing, culturing strong buds and rooting culturing and comprises the steps of collecting underyearling twigs of cyclobalanopsis glauca which is in half lignifications and grows robustly as the explant, pruning and sterilizing the explant, inoculating the explant into an initial bud inducing and culturing medium to obtain the initial buds, then inoculating the initial buds into a multiplication culturing medium, forming cluster buds after 3 to 4 periods of multiplication culture, inoculating the cluster buds into a strong bud culture medium to be cultured and finally inserting single buds with the height larger than or equal to 2cm into a rooting culture medium to be induced and rooted. The rapid propagation method disclosed by the invention shortens a seedling culture period of the cyclobalanopsis glauca, saves seedling culture materials, overcomes the problems that a traditional seedling culture method needs a lot of seedling culture materials and has a long period, greatly reduces production cost, improves production efficiency and has better economical benefit, social benefit and ecological benefit.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to a rapid propagation method for the tissue culture of qinggang tree. Background technique [0002] Cyclobalanopsis glauca (Thunb.) Oerst.), belonging to the Fagaceae Quercus plant, is a deciduous or evergreen tree, with yellow-green flowers in May, unisexual flowers, monoecious plants, male flowers catkins, slender sagging. Nuts are ovoid or oval, born in cup-shaped shells, and mature in October. The leaves will change with the weather, so it is also called "weather tree". It is a subtropical tree species and one of the most widely distributed tree species in my country. North Korea, Japan, and India are also distributed. Neutral light-loving, slightly tolerant to side shade when young. It likes to grow on slightly alkaline or neutral limestone soil, and it also grows well on acidic soil. Deep-rooted tap root system, resistant to drying, can grow in rocky and gravel ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001A01H4/008
Inventor 唐春艳陈素云
Owner 唐春艳
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