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Rapid propagation method for cyclobalanopsis glauca tissue culture

A technology of tissue culture and qinggang tree, which is applied in the field of plant propagation, can solve the problems of slow growth and low bud multiplication coefficient, and achieve the effects of large yield of subculture buds, simple operation process and reduced production cost

Inactive Publication Date: 2017-06-06
唐春艳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for tissue culture and propagation of twigs with good growth effect and fast propagation for the problems of low bud proliferation coefficient and slow growth in the tissue culture process of qinggang tree

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Selection of explants: Collect semi-lignified and vigorously growing twigs of Pleurotus sativa in the same year as explants;

[0026] (2) Treatment of explants: Rinse the explants under tap water for 10 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 15 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0027] (3) Initial bud induction culture: Inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 500 lx, and culture at 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modified WPM me...

Embodiment 2

[0033] (1) Selection of explants: Collect semi-lignified and vigorously growing twigs of Pleurotus sativa in the same year as explants;

[0034] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0035] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modif...

Embodiment 3

[0041] (1) Selection of explants: Collect semi-lignified and vigorously growing twigs of Pleurotus sativa in the same year as explants;

[0042] (2) Treatment of explants: Rinse the explants under tap water for 15 minutes, then remove the leaves of the explants, then soak the explants in a mercuric chloride solution with a volume concentration of 0.1%, and control the soaking time for 20 minutes. Finally, wash the medicine with pure water, cut the explants into stem segments with at least 1 axillary bud and a length of 1.5-3 cm, and put them in containers according to the degree of lignification of the stem segments, and put them in containers for later use;

[0043] (3) Initial bud induction culture: inoculate the explants obtained in step (2) into the initial bud induction medium for culture, place them in light culture, light 1000 lx, and culture temperature 20±1°C for 30-35 d, initial bud germination and growth; the formula of the initial bud induction medium is: 1 / 2 modif...

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PUM

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Abstract

The invention discloses a rapid propagation method for cyclobalanopsis glauca tissue culture. The rapid propagation method comprises the work procedures of selecting explant, processing the explant, inducing and culturing initial buds, multiplication culturing, culturing strong buds and rooting culturing and comprises the steps of collecting underyearling twigs of cyclobalanopsis glauca which is in half lignifications and grows robustly as the explant, pruning and sterilizing the explant, inoculating the explant into an initial bud inducing and culturing medium to obtain the initial buds, then inoculating the initial buds into a multiplication culturing medium, forming cluster buds after 3 to 4 periods of multiplication culture, inoculating the cluster buds into a strong bud culture medium to be cultured and finally inserting single buds with the height larger than or equal to 2cm into a rooting culture medium to be induced and rooted. The rapid propagation method disclosed by the invention shortens a seedling culture period of the cyclobalanopsis glauca, saves seedling culture materials, overcomes the problems that a traditional seedling culture method needs a lot of seedling culture materials and has a long period, greatly reduces production cost, improves production efficiency and has better economical benefit, social benefit and ecological benefit.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and relates to a rapid propagation method for the tissue culture of qinggang tree. Background technique [0002] Cyclobalanopsis glauca (Thunb.) Oerst.), belonging to the Fagaceae Quercus plant, is a deciduous or evergreen tree, with yellow-green flowers in May, unisexual flowers, monoecious plants, male flowers catkins, slender sagging. Nuts are ovoid or oval, born in cup-shaped shells, and mature in October. The leaves will change with the weather, so it is also called "weather tree". It is a subtropical tree species and one of the most widely distributed tree species in my country. North Korea, Japan, and India are also distributed. Neutral light-loving, slightly tolerant to side shade when young. It likes to grow on slightly alkaline or neutral limestone soil, and it also grows well on acidic soil. Deep-rooted tap root system, resistant to drying, can grow in rocky and gravel ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001A01H4/008
Inventor 唐春艳陈素云
Owner 唐春艳
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